PR1MA™ Taq

• Ideal for routine PCR applications as well as genotyping, colony PCR, and fast PCR
• Improved template affinity and solubility for higher enzyme activity and greater yields
• Proprietary buffer optimized for a variety of assay conditions
• Conveniently supplied as a 2X Master Mix or in a 2-tube format of polymerase and separate 5X buffer

PR1MA™ Taq provides superior results for routine applications. Modified to improve DNA-binding, this polymerase offers higher solubility and greater template affinity, resulting in consistently superior performance. PR1MA™ Taq Polymerase exhibits a 5' to 3' nuclease activity, but no 3' to 5' (proofreading) activity and works well with a wide range of DNA templates including GC-rich sequences.

The polymerase is supplied with a 5X buffer containing MgCl2 and a proprietary mix of enhancers (dNTP's not included). For convenience, PR1MA Taq is also available in a ready-to-use 2X Master Mix - just add primers and template DNA. Our "Red Dye" Master Mix incorporates a red-loading dye that allows amplified samples to be loaded directly on an agarose gel.  The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.

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PR1MA™ SmartGlow™ DNA Safe Stain

• Replaces hazardous Ethidium Bromide (EtBr)
• Better sensitivity than EtBr
• Detect as little as 0.1ng of DNA
• Two types available
• PS Pre Stain
• LD Loading Dye
• Excitation by UV light or blue light
• Compatible with Accuris SmartBlue™ Transilluminator
• Ships at ambient temperature (stored at 4°C)

PR1MA™ SmartStain™ PS (Pre-Stain) can be used as a direct replacement for Ethidium Bromide in agarose and polyacrylamide gel electrophoresis. The stain emits green fluorescence when bound to dsDNA or ssDNA and emits red fluorescence when bound to RNA. PR1MA™ SmartStain™ PS exhibits excitation peaks at 290 nm and 490 nm, allowing it to be used with UV and blue light.

Protocol:

• Prepare 100 mL of agarose or polyacrylamide solution.
• Add 5 uL of PR1MA™ SmartStain™ to the gel solution before pouring gels.
• For enhanced results, add PR1MA™ SmartStain™ PS to the running buffer at a ratio of 5 uL per 100 mL. Adding PR1MA™ SmartStain™ PS to the running buffer will result in increased sensitivity and better detection of small quantities of nucleic acid.
• After electrophoresis is complete, view the gel using a UV or blue light illuminator

PR1MA™ Taq Plus

• Higher fidelity for long PCR amplicons, up to 35 kb
• Ideal for problematic templates
• Better sensitivity and higher activity for low-copy and long PCR
• Enzyme of choice for TA cloning

PR1MA™ Taq Plus, an optimized blend of our Hot Start Taq and a proofreading polymerase, provides 5X better fidelity than wild-type Taq and increased enzyme activity. It is the perfect choice for GC and AT rich templates, low copy number, and long PCR.

PR1MA™ Taq Start Taq DNA Polymerase 

PR1MA™ Taq Plus Master Mix, 2X Concentration

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• Convenient cDNA synthesis and PCR in a single tube from 1 pg total RNA
• Formulated for highly specific and sensitive RT-PCR from any RNA templates
• Incorporates thermostable reverse transcriptase and Hot-Start Taq for preparation at room temperature

The PR1MA™ One-Step RT-PCR Kit has been formulated for cDNA synthesis and subsequent PCR in a single tube for end-point analysis. This latest generation RT-PCR Kit consists of an MMLV-derived, thermostable Reverse Transcriptase (45°C to 55°C), an advanced RNase Inhibitor and PR1MA™ Hot Start Taq for ultra-sensitive one-step RT-PCR from as little as 1pg total RNA starting material.

The optimized buffer chemistry allows for efficient reverse transcription and PCR of problematic sequences with significant secondary structure (such as GC-rich targets). The PR1MA One-Step RT-PCR Kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative analysis of RNA transcription levels. The kit also efficiently synthesizes double-stranded cDNA for subsequent gene expression analysis.

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 Item PR2110-100 has been discontinued by the manufacturer;
however, we will be continuing to supply the other quantities.

 

In place of item PR2110-100, please order items PR2110-50 or PR2110-200

PR1MA™ qMAX™ cDNA Synthesis Kits

PR1MA™ now offers two cDNA Synthesis Kits, to meet a range of requirements.
 
The original cDNA Synthesis Kit is a 2-tube format for easy reaction setup and is ideal for 4 pg to 0.5 µg of input RNA. One tube includes our exceptionally stable Reverse Transcriptase combined with a potent RNAse inhibitor, and the other tube contains a 5X reaction buffer with an optimal mixture of anchored oligo (dT) primers and random hexamers to produce a non-biased population of cDNA. 
 
The 1st Strand cDNA Synthesis Flex Kit includes four components: a high-capacity Reverse Transcriptase, an optimized 5X buffer, separate solutions of oligo (dT) primers, and random hexamer primers. This multi-component format is ideal for 10 pg to 2.0 µg of input RNA and allows for greater flexibility in assay design.

 
PR1MA™ qMax cDNA Synthesis Kits
  

Item	Description	Volume (20 µL)
PR2100-C-S	PR1MA™ qMax™ cDNA Synthesis Kit	10 Reactions (Sample)
PR2100-C-25	PR1MA™ qMax™ cDNA Synthesis Kit	25 Reactions
PR2100-C-100	PR1MA™ qMax™ cDNA Synthesis Kit	100 Reactions
PR2100-C-250	PR1MA™ qMax™ cDNA Synthesis Kit	250 Reactions

 

PR1MA™ qMax First Strand cDNA Synthesis Flex Kits  
 

Item	Description	Volume (20 µL)
PR2110-S	PR1MA™ qMax™ First Strand cDNA Synthesis Flex Kit	10 Reactions (Sample)
PR2110-50	PR1MA™ qMax™ First Strand cDNA Synthesis Flex Kit	50 Reactions
PR2110-100	PR1MA™ qMax™ First Strand cDNA Synthesis Flex Kit	100 Reactions
PR2110-200	PR1MA™ qMax™ First Strand cDNA Synthesis Flex Kit	200 Reactions

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PR1MA™ dNTPs

• Supplied as a ready-to-use 40 mM mix or a set of 4 separate 100 mM solutions
• Free of impurities and inhibitors that reduce sensitivity and yield
• No nuclease, protease or nickase activity
• >99% pure, purified by HPLC
• 24-month shelf life

PR1MA™ dNTP's are purified by HPLC in a strict process that results in greater than 99% purity. The stringent purification process eliminates PCR inhibitors such as tetraphosphates and pyrophosphates that can interfere with the sensitivity of your PCR and reduce yields.
The 40 mM dNTP Mix is a single tube that contains premixed dNTPs at a concentration of 10 mM each. The 100 mM dNTP Set contains four individual tubes, one each of dATP, dCTP, dGTP and dTTP. The nucleotides are supplied in ultra-pure water as an ammonium salt. Both the set and mix are stable for 24 months when stored at -20ºC.

Extensive quality control testing is performed to ensure the dNTPs are free of nuclease, protease and nickase activity. Each lot is performance tested in standard PCR, long PCR and qPCR reactions to assess reproducibility and sensitivity.

PR1MA™ dNTP Mix

PR1MA™ dNTP Set  

Item	Description	Volume
PR3101-S-1	PR1MA™ 100 mM dNTP Set, dATP, dCTP, dGTP, dTTP	250 uL / 25 umol each
PR3101-S-4	PR1MA™ 100 mM dNTP Set, dATP, dCTP, dGTP, dTTP	1 mL / 100 umol each
PR3101-S-20	PR1MA™ 100 mM dNTP Set, dATP, dCTP, dGTP, dTTP	20 x 250 uL / 25 umol each

 

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 PR1MA™ Hot Start Taq

• Exceptional sensitivity for low-copy PCR
• Ideal for multiplex PCR and amplification of GC-rich DNA
• Same enhanced features as PR1MA Taq Polymerase
• Buffer optimized for fast cycling and reproducibility 

Engineered for controlled polymerase activity, PR1MA™ Hot Start Taq is bound with a monoclonal antibody that blocks enzyme activity. This allows reactions to be set up at room temperature without the risk of non-specific amplification.

When samples are ready, the reaction mixture is heated to 95°C to denature the antibody and initiate the reaction. Similar to the standard PR1MA™ Taq, Hot Start Taq is provided with a 5X buffer, or in a ready-to-use 2X Master Mix. The Master Mix can be ordered with or without an incorporated red gel loading dye. The Red Dye Master Mix incorporates a red-loading dye that allows amplified samples to be loaded directly on an agarose gel.  The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.

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PR1MA™ High Fidelity Polymerase

• 50X higher fidelity than Taq DNA polymerase
• Works with crude DNA sample
• Ideal for cloning, mutagenesis and microarrays
• Produces blunt end products, to clone directly into blunt end vectors
• Optimized buffer system with unique PCR enhancers 

For applications requiring highly accurate amplification, choose PR1MA™ High Fidelity DNA polymerase. Modified for better solubility and higher activity across a broad range of ionic conditions, this polymerase will amplify a wide range of targets, including those that are GC or AT rich as well as crude samples.

A 3'-5' proofreading exonuclease activity and an error rate of 4.55×10-7 makes PR1MA™ High Fidelity DNA Polymerase the perfect partner for cloning applications. The supplied 5X buffer with dNTPs is optimized for compatibility with a variety of targets.

Specifications

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PR1MA™ High Fidelity Hot Start Master Mix

• Leaves an A-overhang for TA cloning
• Ideal for difficult, high GC content sequences
• 10x fidelity of native Taq
• Ideal for long PCR, up to 10kb targets 

PR1MA™ High Fidelity Hot Start Mix is a hot start 2x formulation which provides excellent sensitivity in low-copy number assays with 10x higher fidelity than Taq polymerase. The 2x master mix contains proprietary enhancers, an antibody-mediated hot-start mechanism, and a proofreading component for trouble-free PCR reaction assembly and performance.

The pre-optimized Hot Start Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation and hot-start blend provide the ideal conditions for high-performance PCR and inactivity at room temperature thereby eliminating non-specific amplification.

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PR1MA™  High Fidelity Master Mix

• Leaves a blunt end
• Rapid extension: up to 1 kb per 15 seconds
• 100x fidelity of native Taq
• Ideal for shorter, less complex targets

A 2x formulation which provides extreme sensitivity in low copy number assays with 100x the fidelity of native Taq, PR1MA™ High Fidelity Master Mix is perfect for shorter, less complex targets. The 2x master-mix contains proprietary enhancers and a proof-reading component for trouble-free PCR reaction assembly and performance. High Fidelity Master Mix delivers a unique balance of PCR sensitivity, high fidelity, versatility, and tolerance to inhibitors.

The pre-optimized Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation provides the ideal conditions for high-performance PCR.

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PR1MA™ Mammalian Genotyping Kit

• DNA extraction and amplification in 1 hour
• Proprietary lysis buffer optimized for ear punches, tail snips and other mammalian tissues
• Single tube - no organic solvents or clean up procedures
• Enhanced PR1MA™ Hot Start Polymerase included

Traditionally, mammalian genotyping protocols have involved Proteinase K digestion, neutralization, organic extraction and lots of hands on time to get PCR-ready DNA.

The PR1MA™ Mammalian Genotyping Kit is quick and easy to use - add sample to the proprietary lysis buffer and incubate for 5-10 minutes, then add the deactivation buffer and incubate for 10 minutes. The crude lysate can then be amplified using fast PCR PR1MA™ Hot Start Taq Master Mix with red loading dye (included). The kit contains everything needed - just add sample and primers. 
 

PR1MA™ 1 Hour Mammalian Genotyping Kit 

 PR1MA™ Genotyping Hot Start Master Mix, 2X Concentration with Red Dye

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PR1MA™ Tn5 2.0 Transposase

PR1MA™ Tn5 2.0 Transposase (Tnp) is a hyperactive retroviral integrase engineered for improved activity, speed, and robustness that is used to construct random next-generation sequencing libraries and to study chromatin structure using targeted ATAC-seq. PR1MA™ Tn5 2.0 can be used to randomly fragment any target and insert unique oligonucleotide adapters in a single reaction which reduces the time and sample requirements relative to traditional next-gen sequencing library construction. 

• Optimal temperature: 55°C
• Inactivation: 40X Stop solution included (2% SDS)
• Storage temperature: -20°C
• 10X Tn5 reaction buffer included

Buffer composition

• 100 mM Tris-HCl
• 100 mM MgCl2
• pH = 7.5

*These products are intended for research use only, not for diagnostic use. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.   
 

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