PR1MA™ Tryptone, Granulated

CAS Number: 91079-40-2
Solubility: Water
Storage Temp: Room Temperature

Pancreatic digest of casein. Material is specially granulated to reduce dust and increase solubility rate.

Ingredients:

• Total Nitrogen (TN): ~12.7%
• Amino Nitrogen (AN): ~3.7%
• AN/TN Ratio: 0.26
• Sodium Chloride: ~0.4%
• Tryptophan: 1.0
• pH (1% solution): ~7.3%

• Appearance: Light Beige
• Amino Nitrogen Content: ≥ 3.5 %
• Ash: ≤ 15.0 %
• Loss on Drying: ≤ 5.0 %
• pH (2.0% Solution @ 25 ^oC): 6.5 - 7.5
• Total Nitrogen Content: ≥ 10 %
• Enzyme Species: Porcine
• Raw Material Species: Bovine

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PR1MA™ Luria Broth Granulated [Miller's LB Broth]

Common Name: Miller's LB Broth
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temp: Room Temperature

For propagation and maintenance of E. Coli. High Salt Formula. Molecular Biology Grade.

Dissolve 25 grams in 1 liter distilled water and adjust the pH to 7.2. Heat and frequently agitate to completely dissolve. Sterilize by autoclaving for 15 minutes. Material specially granulated to reduce dust and increase solubility rate.

Ingredients:

• Tryptone:10 g/L
• Sodium Chloride: 10 g/L
• Yeast Extract: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Cream to beige granular powder
• DCM Appearance: Homogeneous, free flowing beige to yellow-beige
• PPM Appearance: Clear, yellow to amber no precipitate (ppt)
• Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
• pH (2.5% H₂O @25 °C): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Casein Peptone: 10 g/L
• Sodium Chloride: 10 g/L
• Yeast Extract: 5 g/L

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PR1MA™ Yeast Extract, Granulated

CAS Number: 8013-01-2
Solubility: Water
Storage Temp: Room Temperature

Dried yeast autolysate with a high content of amino nitrogen and water soluble B complex vitamins. Material is specially granulated to reduce dust and increase solubility rate.

Suitable for Microbiology Applications.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Light Beige Powder
• Amino Nitrogen Content: ≥ 3.5 %
• Dry Matter: ≥ 94 %
• Moisture : ≤ 6.0 %
• pH (2.0% Solution @ 25 ^oC): 6.5 - 7.5
• Sodium Chloride: ≤ 0.5 %
• Total Nitrogen Content: ≥ 8.0 %
• Non Animal Origin: The product does not come in contact with materials of animal origin.

Yeast, Extract, Granulated,PR1MA, MIDSCI, Laboratory Supplies, High-Quality, Research, Consumables, Reagents, Equipment, Customer Service, Quick Shipping, Satisfaction Guarantee, Personalized Solutions, Universities

PR1MA™ 2XYT Broth, Granulated

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temp: Room Temperature

Nutritionally enriched medium for the growth of recombinant strains of E. Coli and in the preparation and transformation of competent cells.

Material is specially granulated to reduce dust and increase solubility rate.

Dissolve 31g in one liter distilled water. Heat solution to boiling with continuous stirring. Sterilize by autoclaving at 121°C for 15 minutes. Final pH@ 25°C: 6.8-7.2.

Ingredients:

• Digest Peptone: 16 g/l
• Yeast Extract: 10 g/l
• Sodium Chloride: 5 g/l

Research or further manufacturing use only, not for food or drug use.

• Appearance: Cream to beige free flowing granulated
• DCM Appearance: Homogeneous free flowing, beige
• PPM Appearance: Clear,no to light ppt,yellow
• pH (3.1 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

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PR1MA™ Peptone, Granulated

CAS Number: 73049-73-7
Solubility: Water
Storage Temp: Room Temperature

Mixtures of peptides and free amino acids from peptic digest of animal tissue.

• Amino Nitrogen (AN): 2.5-3.5%
• Loss on Drying: <6.0%

Research or further manufacturing use only, not for food or drug use.

• Appearance: Light Beige Granular Powder
• Amino Nitrogen Content: ≥ 3.4 %
• Appearance of Solution: Clear and yellow-beige
• Ash: ≤ 15.0 %
• Loss on Drying: ≤ 5.0 %
• pH (2.0% Solution @ 25 ^oC): 6.5 - 7.5
• Total Nitrogen Content: ≥ 10.0 %

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PR1MA™ Agar, Granulated, High Gel Strength

CAS Number: 9002-18-0
Solubility: Water with Heat
Storage Temp: Room Temperature

Purified agar with excellent properties for tissue culture and microbiological work.

• To prepare media, add 15 grams to 1 liter of water.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Cream Color, Average Particle Size Between 1-2 mm
• Granulometry (ASTM) 60 Mesh Sieve Size: Report %
• Ash: ≤ 6.5 %
• E.Coli: Negative
• Gel Point (1.5% Gel): 33 - 38°C
• Gel Strength (1.5% Gel): 600 - 1100 g/cm²
• Melting Point: 88 - 96°C
• Moisture : ≤ 15 %
• Molds and Yeasts: < 100 cfu/g
• pH: 6.0 - 8.0
• Total Plate Count: < 1000 cfu/g
• Viscosity (1.5 Solution @ 60 ^oC): ≤ 30 cps
• Water Absorption: ≤ 75 cc

PR1MA™ LB Agar, Low Salt Formula, Granulated [Lennox L Agar]

Common Name: Lennox L Agar
CAS Number: 91079-40-2, 8013-01-2, 7647-14-5, 9002-18-0
Solubility: Water with Heat
Storage Temp: Room Temperature

For propagation and maintenance of E. Coli.

Dissolve 32 grams in 1 Liter of distilled water and adjust the pH to 7.2. Sterilize by autoclaving for 15 minutes.

Ingredients:

• Casein Peptone: 10 g/L
• Sodium Chloride: 5 g/L
• Yeast Extract: 5 g/L
• Agar: 12 g/L

• DCM Appearance: Homogeneous Free Flowing ,Light Beige
• PPM Appearance: Clear, no to light ppt, light yellow
• pH (2.0% Solution @ 25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Sodium Chloride: 5 g/l
• Yeast Extract: 5 g/L
• Agar: 12 g/l
• Casein Peptone: 10 g/L

PR1MA™ LB Broth, Granulated [Lennox LB Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temp: Room Temperature

For propagation and maintenance of E. Coli. Low Salt Formula. Molecular Biology Grade.

Dissolve 20 grams in 1 liter distilled water. Then adjust the pH to 7.2. Heat and frequently agitate to completely dissolve. Material is specially granulated to reduce dust and increase solubility rate. Sterilize by autoclaving for 15 minutes.

Ingredients:

• Tryptone:10 g/L
• Sodium Chloride: 5 g/L
• Yeast Extract: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Light beige color, average particle size between 1 to 2mm
• DCM Appearance: Homogeneous, free flowing light beige
• PPM Appearance: Clear, no to light ppt, light yellow
• Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
• pH (2.0% Solution @ 25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Casein Peptone: 10 g/L
• Sodium Chloride: 5 g/L
• Yeast Extract: 5 g/L

PR1MA™  Luria Agar Granulated [Miller's LB Agar]

Common Name: Miller's LB Agar
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 9002-18-0
Solubility: Water with Heat
Storage Temp: Room Temperature

For propagation and maintenance of E. Coli.

Dissolve 37 grams in 1 Liter distilled water and adjust the pH to 7.2. Sterilize by autoclaving for 15 minutes.

Ingredients:

• Tryptone: 10 g / L
• Sodium Chloride: 10 g / L
• Yeast Extract: 5 g / L
• Agar: 12 g / L

• DCM Appearance: Homogeneous Free Flowing, Light Beige
• PPM Appearance: Clear, none to light ppt, light yellow
• pH (2.5 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™ Terrific Broth, Modified, Granulated

CAS Number: 91079-40-2; 8013-01-2; 7778-77-0; 7758-11-4
Solubility: Water
Storage Temp: Room Temperature

Enriched media for maintenance and propagation of E. Coli and plasmid growth. Produces higher cell densities resulting in greater yields of DNA or recombinant proteins. Glycerol is used as a carbon source with this formulation instead of glucose as it will not ferment to acetic acid.

Ingredients:

• Casein Digest Peptone: 12g/L
• Yeast Extract: 24g/L
• Dipotassium phosphate: 9.5g/L
• Monopotassium phosphate: 2.2g/L

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous, free flowing beige to yellow-beige
• PPM Appearance: Clear, yellow to amber no precipitate (ppt)
• pH (4.76% solution at 25°C): 6.8 - 7.4
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent