PR1MA™ Tricine, Ultra Pure [N-[Tris (Hydroxymethyl) Methyl] Glycine]

Common Name: N-[Tris (Hydroxymethyl) Methyl] Glycine
CAS Number: 5704-04-1
Molecular Weight: 179.17
Chemical Formula: C₆H₁₃NO₅
Solubility: Water
Storage Temp: Room Temperature

• pKa @ 25°C: 8.1-8.2
• Melting Range: 182-188°C

• Appearance: White Crystalline Powder
• Assay (Titration, dried basis): ≥ 99.0 %
• Heavy Metals : ≤ 5 ppm
• pH (1% Solution): 4.6 - 5.3
• Solubility (1M Solution in H₂O): Clear
• UV Absorbance 260nm (1 mol/L, 1cm ): ≤ 0.04
• UV Absorbance 280nm (1 mol/L, 1cm ): ≤ 0.02
• Water Content : ≤ 0.1 %

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PR1MA™ TBE 10x Solution

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PR1MA™ TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl]

Common Name: Tris(hydroxymethyl) aminomethane hydrochloride
CAS Number: 1185-53-1
Molecular Weight: 157.6
Chemical Formula: C₄H₁₁NO₃ • HCl
Solubility: Water
Storage Temp: Room Temperature

Highly purified for use in molecular biology applications.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White crystalline
• Assay: 99.0 - 101.0 %
• pH (1% aqueous solution): 4.2 - 5.0
• Solubility (1M Solution in Water ) : Clear, colorless solution
• UV Absorbance @260nm, 1.0 M in H₂O: ≤ 0.06
• UV Absorbance @280nm, 1.0 M in H₂O: ≤ 0.05
• Identification: Conforms
• Heavy Metals : ≤ 5 ppm
• Grade: USP

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PR1MA™ TBE Buffer 10x Powder

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Tris Hydrochloride

• Appearance: White, Crystalline Powder
• Purity: ≥ 99.0%
• pH (1% Aqueous): 4.2 - 5.0
• Solubility (1M Aqueous): Clear and Colorless
• Identity (FTIR): Conforms to Structure
• Loss on Drying (105°C/4h): ≤ 0.5%
• Melting Point: 149ºC - 153ºC
• A260 (40% Aqueous): ≤ 0.06
• A280 (40% Aqueous): ≤ 0.05
• Lead: 5 ppm
• RNase: None Detected
• DNase: None Detected

*Custom sizes available upon request.

DOT Information: Non-regulated.   
 

Tris Base

• Appearance: White, Crystalline Powder
• Purity: ≥ 99.5%
• pH (5% Aqueous): 10.0 - 11.5
• Solubility (40% Aqueous): Clear and Colorless
• Identity (FTIR): Conforms to Structure: ≤ 0.5%
• Loss on Drying (105°C/4h): ≤ 0.5%
• Melting Point: 168ºC – 172ºC
• A280 (40% Aqueous): ≤ 0.07
• A400 (40% Aqueous): ≤ 0.02
• Lead: ≤ 5 ppm
• Iron: ≤ 5 ppm
• Chloride: ≤ 3 ppm
• Sulfate: ≤ 10 ppm
• RNase: None Detected
• DNase: None Detected

*Custom sizes available upon request.

DOT Information: Non-regulated.   
 

Electrophoresis Buffers
QuickSilver - TAE (Tris-acetate-EDTA)

TAE is ideal for separation of large DNA fragments in agarose gels and is used as running buffer and in gel preparation.

TAE is the buffer of choice when recovering DNA from gels and when downstream processing involves enzymatic reactions. Final concentration of a 1X working solution is 40mM Tris, 20mM acetic acid, 1mM EDTA.

QS1000 QuickSilver TAE Buffer, 1X, 100 packs

QuickSilver - TBE (Tris-borate-EDTA)

TBE is used in gel preparation and as a running buffer for electrophoresis of nucleic acids. TBE works best for resolution of smaller fragments (<2kb). Its higher buffering capacity makes it useful for longer runs. The final concentration of a 1X working solution is 89mM Tris, 89mM boric acid, 2mM EDTA.

QS1001 QuickSilver TBE Buffer, 1X, 50 packs

QuickSilver - Fast Running Buffer

QuickSilver Fast Running Buffer is a proprietary blend formulated for agarose gel electrophoresis at high voltage (300-350V). Its strong buffering capacity minimizes heat generation all allows for extremely fast separation of fragments smaller than 5kb. A typical 30-minute run can be performed in just 10 minutes using this buffer.

QS1002 QuickSilver Fast Running Buffer, 1X, 100 packs
 
 

Protein Electrophoresis Buffers

QuickSilver™ TGS (Tris-Glycine-SDS)

Tris-Glycine-SDS is one of the most common running buffers used for SDS-PAGE. A 1X solution contains 25mM Tris, 192mM Glycine and 0.1% SDS.

QS1100 QuickSilver Tris Glycine-SDS Buffer, 1X, 50 packs

QuickSilver™ Tris-MES-SDS

Small to medium molecular weight proteins can be resolved on SDS-PAGE gels using Tris-MES-SDS as a running buffer. Proteins resolve faster in gels using Tris-MES-SDS as a running buffer than those using Tris-MOPS-SDS. A 1X solution has a final concentration of 50mM Tris, 50mM MES, 0.1% SDS and 1mM EDTA.

QS1101 QuickSilver Tris-MES-SDS Buffer, 1X, 50 packs

QuickSilver™ Tris-MOPS-SDS

Tris-MOPS-SDS is ideal for use as a running buffer when resolving medium to large proteins with SDS-PAGE. Gels run with Tris-MOPS-SDS buffer run slower than those using Tris-MES-SDS buffer. The final concentration of a 1X working solution is 50mM Tris, 50mM MOPS, 0.1% SDS and 1mM EDTA.

QS1102 QuickSilver Tris-MOPS-SDS, 1X, 50 packs

Western Blotting, ELISA, Cell Culture, Chromatography

QuickSilver™ PBS and PBST

Because its osmolality and ion concentration closely mimic that of the human body, PBS is commonly used for cell culture applications, as well as HPLC and MS. PBST is a wash buffer and diluent for ELISA. The 1X PBS solution contains 137mN NaCl, 2.7mM KCl and 10mM phosphate. PBST is supplemented with 0.05% Tween-20.

QS1200 QuickSilver PBS Buffer, 1X, 100 packs

QS1201 QuickSilver PBST Buffer, 1X, 100 packs

QuickSilver™ TBS and TBST

TBS and TBST are used to maintain pH within a very narrow range for a variety of applications including western blotting and immunoassays. The final concentration of a 1X working solution is 137mM NaCl, 2.7mM KCl, 50mM Tris. In addition, 1X TBST contains 0.1% Tween-20.

QS1202 QuickSilver TBS buffer, 1X, 100 packs

QS1203 QuickSilver TBST buffer, 1X, 100 packs

PR1MA™  TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl]

Common Name: Tris(hydroxymethyl) aminomethane hydrochloride
CAS Number: 1185-53-1
Molecular Weight: 157.6
Chemical Formula: C₄H₁₁NO₃ • HCl
Solubility: Water
Storage Temp: Room Temperature

Highly purified for use in molecular biology applications.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White crystalline
• Assay: 99.0 - 101.0 %
• pH (1% aqueous solution): 4.2 - 5.0
• Solubility (1M Solution in Water ) : Clear, colorless solution
• UV Absorbance @260nm, 1.0 M in H₂O: ≤ 0.06
• UV Absorbance @280nm, 1.0 M in H₂O: ≤ 0.05
• Identification: Conforms
• Heavy Metals : ≤ 5 ppm
• Grade: USP

PR1MA™  Tris Base Ultra Pure [Tris (Hydroxymethyl) Aminomethane]

Common Name: Tris (Hydroxymethyl) Aminomethane
CAS Number: 77-86-1
Molecular Weight: 121.1
Chemical Formula: C₄H₁₁NO₃
Solubility: Water
Storage Temp: Room Temperature

Highly purified for use in molecular biology applications.

Moisture: <0.2%
Color: <20 APHA (colorless)
Heavy Metals (as Pb): <0.0001%
Insoluble Matter: <0.005%
Heavy Metals: 5 ppm
pKa (20°C): 8.1 ± 0.1

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Crystalline Powder
• Purity (Dried Basis By GC): ≥ 99.9 %
• Related Substances By GC: ≤ 0.1 %
• Water: ≤ 0.2 %
• Loss on Drying @ 105 ^oC, 3hrs: ≤ 1.0 %
• Melting Point: 170 - 172 ^oC
• APHA Color, 20% Aqueous solution: ≤ 20
• Insoluble Matter: ≤ 0.005 %
• Heavy Metals (as Pb): ≤ 5 ppm
• Residue on Ignition: ≤ 0.5 %
• Iron (Fe): ≤ 5 ppm
• UV Absorbance @260nm, 10% Aqueous solution : ≤ 0.03
• UV Absorbance @280nm, 10% Aqueous solution : ≤ 0.02
• UV Absorbance @290nm, 40% solution in H₂O: ≤ 0.20
• UV Absorbance @430nm, 10% Aqueous solution : ≤ 0.004
• Identification A: Pass
• Identification B: Pass
• Identification C: Pass
• pH (5% in water): 10.0 - 11.5
• Arsenic (As): ≤ 1 ppm
• Chloride: ≤ 100 ppm
• Copper (Cu): ≤ 1 ppm
• DNase, RNase, protease: None Detected
• Lead: ≤ 1 ppm
• Magnesium (Mg): ≤ 1 ppm
• pH (1M in H₂O): 10.5 - 11.5
• Residual Solvents: Conforms to USP/EP
• Residue on Ignition (USP): ≤ 0.1 %
• Solubility (2M In H₂O): Pass

PR1MA™  TAE 10X Solution [TRIS Acetate EDTA 10X Solution]

Common Name: TRIS Acetate EDTA 10X Solution
CAS Number: 77-86-1, 64-19-7, 6381-92-6, 7732-18-5
Solubility: Water
Storage Temp: Room Temperature

10X solution contains:

• TRIS-Base: 0.4 M
• Acetic Acid: 0.2 M
• EDTA: 10 mM
• pH (10X Solution (@25°C): 8.2-8.4

• Appearance: Clear, Colorless Solution
• pH: 7.8 - 8.2

PR1MA™ Tris-Acetate

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