PR1MA™  HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)]

Common Name: N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
CAS Number: 75277-39-3
Molecular Weight: 260.3
Chemical Formula: C₈H₁₇N₂O₄SNa
Solubility: Water
Storage Temp: Room Temperature

• pKa (20°C): 7.45-7.65
• pH Range: 6.8-8.2

Research or further manufacturing use only, not for food or drug use.

• Appearance: White crystalline powder
• Assay (Titration, dried basis): ≥ 98.0 %
• Chloride (Cl): ≤ 0.1 %
• Heavy Metals (as Pb): ≤ 1 ppm
• Loss on Drying @ 105 ^oC, 3hrs: ≤ 2.0 %
• pH (1% aqueous @ 25 ^oC): 9.4 - 11.0
• pKa @ 20°C: 7.45 - 7.65
• Solubility (1M Solution in H₂O): Clear, complete and colorless
• UV Absorbance @ 250nm 1M: ≤ 1.0

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PR1MA™ Bicine Ultra-Pure

CAS Number: 150-25-4
Molecular Weight: 163.2
Chemical Formula: C₆H₁₃NO₄
Solubility: Water
Storage Temp: Room Temperature

Zwitterionic buffer used in a variety of molecular biology applications such as protein resolution, protein crystallization and SDS-PAGE.

pKa @ 25°C: 8.3
pH Range: 7.6-9.0
Melting Range: 190-192°C

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Powder
• FTIR: Conforms to reference
• Optical ABS @ 260 NM: ≤ 0.1
• Optical ABS @ 280 NM: ≤ 0.1

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Azure HRP Stripping Buffer

Works by removing the primary and secondary antibodies from the membrane

Stripping buffer for stripping and re-probing chemiluminescent Western blots.

• Provided as a 1x ready-to-use solution
• Size: 500mL

Azure Chemi Blot Blocking Buffer

This Chemi Blot Blocking Buffer was formulated to enhance signal and lower background by improving sensitivity. Enhances specific antibody-antigen interactions specifically for chemiluminescent Western blots. 

• Provided as a 1x ready-to-use solution- no mixing or dilutions required.
• Size: 500mL

Features

Western blot membranes should be incubated in blocking buffer before antibody hybridization to prevent non-specific binding of antibodies. Blocking buffer can also be used for diluting primary and secondary antibodies during hybridization.

Azure Blot Washing Buffer

Wash buffers are an important part of the blotting process for removing unhybridized antibody and preventing background signal.

Blot washing buffer for use with Azure Imaging Systems, or any other Western blotting system.

• Provided as a 10x concentrated solution
• Size: 500mL

NOTE: While this Blot Washing Buffer is compatible with NIR Fluorescent Western blots, for the best results with NIR fluorescence, we recommend the Azure Fluorescent Blot Washing Buffer.

Azure Protein-free Blot Blocking Buffer

Protein-free blocking solution optimized for fluorescent Immunoblots and Western blots

Does not contain proteins. Azure Protein-Free Blot Blocking Buffer can reduce background in Western blots where the primary antibodies tend to cross-react with protein blockers like BSA, casein or milk protein. For low-quality primary antibodies that may require protein-based blocking agents, BSA or non-fat dry milk can be dissolved directly in the Azure Protein-Free Blot Blocking Buffer and then used to dilute the antibody.

Best used with assays where animal-based product usage is prohibited. Serves as an excellent blocking reagent for chemiluminescent Western blotting.

• Provided as a 5x solution
• Size: 200mL

Citric Acid, Monohydrate 

• Appearance (Color): Colorless or White
• Appearance (Form): Crystals
• Purity: 99.0 - 102.0%
• Identification (FTIR): Conforms to Structure
• Water: 7.5 - 8.8% 
• Lead: ≤ 3 ppm
• Iron: ≤ 3 ppm
• Oxalate: To Pass Test
• Sulfate: ≤ 20 ppm
• Chloride: ≤ 10 ppm
• Readily Carbonizable Substances: Passes Test
• Insoluble Matter: ≤ 0.005%
• Residue After Ignition: ≤ 0.02%
• DNase: None Detected
• RNase: None Detected

*Custom sizes available upon request.

DOT Information: Non-regulated.
 

PR1MA™ bis-TRIS [bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane]

Common Name: bis(2-Hydroxyethyl)aminotris (hydroxymethyl)methane
CAS Number: 6976-37-0
Molecular Weight: 209.2
Chemical Formula: C₈H₁₉NO₅
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White crystalline powder
• Assay: ≥ 99.0 %
• Identification (IR): Matches reference
• Melting Point: Report ^oC
• pH, 1.0M Aqueous solution @25ºC: 9.5 - 11.0
• pKa @ 20°C: 6.0 - 7.0
• Solubility: Complete and clear
• UV Absorbance @280nm, 1.0 M in H₂O: ≤ 0.40

PR1MA™  HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)]

Common Name: [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)]
CAS Number: 7365-45-9
Molecular Weight: 238.3
Chemical Formula: C₈H₁₈N₂O₄S
Solubility: Water
Storage Temp: Room Temperature

Zwitterionic N-substituted aminosulfonic acid buffer.

• pka (25°C): 7.5
• pH Range (Working): 6.8 - 8.2
• Heavy Metals: <0.001% Max

• Appearance: White Crystalline Powder
• Assay (Titration, dried basis): ≥ 99.5 %
• DNase, RNase, protease: Not Detected
• Endotoxin (0.2% Solution): ≤ 0.25 EU/ml
• Heavy Metals (as Pb): ≤ 0.001 %
• Identification (IR): Pass
• Iron (Fe): ≤ 0.0005 %
• Loss on Drying: ≤ 1.0 %
• pH (5% in water): 5.0 - 6.5
• pKa @ 20°C: 7.35 - 7.70
• Residue on Ignition: ≤ 0.1 %
• Solubility (1M Solution in Water ): Clear and Colorless Solution
• Sulfate: ≤ 0.05 %
• UV Absorbance (1M@260nm): ≤ 0.05
• UV Absorbance (1M@280nm): ≤ 0.05

Citric Acid, Anhydrous

• Appearance (Color): Colorless or White
• Appearance (Form): Crystals or Crystalline Powder
• Purity: ≥ 99.5%
• Identification (FTIR): Conforms to Structure
• Water: ≤ 0.3%
• Lead: ≤ 5 ppm
• Iron: ≤ 5 ppm
• Oxalate: ≤ 100 ppm
• Sulfate: ≤ 150 ppm
• Chloride: ≤ 50 ppm
• Barium: Passes Test
• Residue on Ignition: ≤ 0.05%
• Readily Carbonizable Substances: Passes Test
• Bacterial Endotoxins: ≤ 0.5 IU/mg
• DNase: None Detected
• RNase: None Detected 

*Custom sizes available upon request.

DOT Information: Non-regulated.   
 

RunBlue SDS Running Buffer (NXB50500) a TEO-Tricine buffer system which provides a separation similar to the MOPS buffer used with BIS-TRIS precast gels. It provides enhanced separation of higher molecular weight proteins. The buffer can be for reduced and non-reduced samples.

RunBlue TGS Blot Buffer(NXB82600) has been specially formulated to provide optimal blot transfer of proteins from RunBlue Precast Gels. Supplied as a concentrate should be diluted by a factor of 10 for use with the RunBlue Dual Run and Blot system or a factor of 20 for use with other blotting methods.

PR1MA™ HEPPS [N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)] [EPPS]

Common Name: HEPPS [N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)] [EPPS]
CAS Number: 16052-06-5
Molecular Weight: 252.3
Chemical Formula: C₉H₂₀N₂O₄S
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Crystalline Powder
• Assay (Titration, dried basis): ≥ 99.0 %
• Loss on Drying: ≤ 1.0 %
• pH (1mol/L in Water @ 20 ^oC: 5.0 - 7.0
• Solubility (0.1M in water): Clear and Colorless