Azure Protein-free Blot Blocking Buffer

Protein-free blocking solution optimized for fluorescent Immunoblots and Western blots

Does not contain proteins. Azure Protein-Free Blot Blocking Buffer can reduce background in Western blots where the primary antibodies tend to cross-react with protein blockers like BSA, casein or milk protein. For low-quality primary antibodies that may require protein-based blocking agents, BSA or non-fat dry milk can be dissolved directly in the Azure Protein-Free Blot Blocking Buffer and then used to dilute the antibody.

Best used with assays where animal-based product usage is prohibited. Serves as an excellent blocking reagent for chemiluminescent Western blotting.

• Provided as a 5x solution
• Size: 200mL

Azure Transfer Buffer

This proprietary transfer buffer provides improved detection of low-abundance and post-translationally modified proteins.

High-efficiency protein transfer and increased protein retention on the membrane add up to more sensitive Western blots.

• Provided as a 50x concentrated solution. Sufficient for 50-100 blots
• Size: 500mL

Features

• High performance—increased protein retention on the membrane and fast transfer times result in more sensitive detection of low-abundance and post-translationally modified proteins
• Efficient—enhanced transfer efficiency for proteins of all sizes
• Fast—transfer proteins in less than 20 minutes vs. several hours required with traditional transfer buffers
• Convenient—use your existing wet transfer electrophoresis apparatus

Citric Acid, Monohydrate 

• Appearance (Color): Colorless or White
• Appearance (Form): Crystals
• Purity: 99.0 - 102.0%
• Identification (FTIR): Conforms to Structure
• Water: 7.5 - 8.8% 
• Lead: ≤ 3 ppm
• Iron: ≤ 3 ppm
• Oxalate: To Pass Test
• Sulfate: ≤ 20 ppm
• Chloride: ≤ 10 ppm
• Readily Carbonizable Substances: Passes Test
• Insoluble Matter: ≤ 0.005%
• Residue After Ignition: ≤ 0.02%
• DNase: None Detected
• RNase: None Detected

*Custom sizes available upon request.

DOT Information: Non-regulated.
 

Citric Acid, Anhydrous

• Appearance (Color): Colorless or White
• Appearance (Form): Crystals or Crystalline Powder
• Purity: ≥ 99.5%
• Identification (FTIR): Conforms to Structure
• Water: ≤ 0.3%
• Lead: ≤ 5 ppm
• Iron: ≤ 5 ppm
• Oxalate: ≤ 100 ppm
• Sulfate: ≤ 150 ppm
• Chloride: ≤ 50 ppm
• Barium: Passes Test
• Residue on Ignition: ≤ 0.05%
• Readily Carbonizable Substances: Passes Test
• Bacterial Endotoxins: ≤ 0.5 IU/mg
• DNase: None Detected
• RNase: None Detected 

*Custom sizes available upon request.

DOT Information: Non-regulated.   
 

RunBlue SDS Running Buffer (NXB50500) a TEO-Tricine buffer system which provides a separation similar to the MOPS buffer used with BIS-TRIS precast gels. It provides enhanced separation of higher molecular weight proteins. The buffer can be for reduced and non-reduced samples.

RunBlue TGS Blot Buffer(NXB82600) has been specially formulated to provide optimal blot transfer of proteins from RunBlue Precast Gels. Supplied as a concentrate should be diluted by a factor of 10 for use with the RunBlue Dual Run and Blot system or a factor of 20 for use with other blotting methods.

Tris Hydrochloride

• Appearance: White, Crystalline Powder
• Purity: ≥ 99.0%
• pH (1% Aqueous): 4.2 - 5.0
• Solubility (1M Aqueous): Clear and Colorless
• Identity (FTIR): Conforms to Structure
• Loss on Drying (105°C/4h): ≤ 0.5%
• Melting Point: 149ºC - 153ºC
• A260 (40% Aqueous): ≤ 0.06
• A280 (40% Aqueous): ≤ 0.05
• Lead: 5 ppm
• RNase: None Detected
• DNase: None Detected

*Custom sizes available upon request.

DOT Information: Non-regulated.   
 

Sodium Phosphate Dibasic, Heptahydrate

• Appearance: White to Off-White Powder
• Purity: ≥ 99.0%
• pH (5% Aqueous) : 8.7 - 9.3
• Solubility: Clear and Colorless
• Identity (FTIR): Conforms to Structure
• Loss on Drying (105°C/4 h): 44 - 50%
• Lead: ≤ 10 ppm
• Iron: ≤ 10 ppm
• Chloride: ≤ 10 ppm
• Phosphate: 35.0% - 39.-%
• Sulfate: ≤ 50 ppm
• RNase: None Detected
• DNase: None Detected

*Custom sizes available upon request.

DOT Information: Non-regulated.   
 

Sodium Phosphate Monobasic, Anhydrous

• Appearance (Color): White to Off-White
• Appearance (Form): Crystalline Powder
• Purity: 98.0 - 103.0%
• pH (5% Aqueous): 4.1 - 4.5
• Solubility: Clear and colorless
• Identity (FTIR): Conforms to Structure
• Loss on Drying (105°C/4h): ≤ 0.5%
• Insoluble Matter: ≤ 2.0%
• Lead: ≤ 5 ppm
• Arsenic: ≤ 3 ppm
• Fluoride: ≤ 50 ppm
• Phosphorous: 24.5 - 26.5%
• RNase: None Detected
• DNase: None Detected

*Custom sizes available upon request.

DOT Information: Non-regulated.
 

Tris Base

• Appearance: White, Crystalline Powder
• Purity: ≥ 99.5%
• pH (5% Aqueous): 10.0 - 11.5
• Solubility (40% Aqueous): Clear and Colorless
• Identity (FTIR): Conforms to Structure: ≤ 0.5%
• Loss on Drying (105°C/4h): ≤ 0.5%
• Melting Point: 168ºC – 172ºC
• A280 (40% Aqueous): ≤ 0.07
• A400 (40% Aqueous): ≤ 0.02
• Lead: ≤ 5 ppm
• Iron: ≤ 5 ppm
• Chloride: ≤ 3 ppm
• Sulfate: ≤ 10 ppm
• RNase: None Detected
• DNase: None Detected

*Custom sizes available upon request.

DOT Information: Non-regulated.   
 

Sodium Chloride

• Appearance: White Crystalline Powder
• Purity: 99.0 - 101.5%
• pH (5% Aqueous): 5.0 - 9.0
• Lead: ≤ 5 ppm
• Iron: ≤ 2 ppm
• Insoluble Matter: ≤ 0.005%
• Iodide: ≤ 20 ppm
• Bromide: ≤ 100 ppm
• Phosphate: ≤ 5 ppm
• Sulfate: ≤ 40 ppm
• Barium: To Pass Test
• Calcium: ≤ 20 ppm
• Magnesium: ≤ 10 ppm
• Potassium: ≤ 50 ppm
• Chlorate & Nitrate: ≤ 30 ppm
• RNase: None Detected
• DNase: None Detected

*Custom sizes available upon request.

DOT Information: Non-regulated.   
 

Sodium Phosphate Dibasic, Anhydrous

• Appearance: White Crystalline Powder
• Purity: ≥ 98.0%
• pH (1% Aqueous): 8.4 – 9.6
• Solubility: Clear and Colorless
• Identity (FTIR): Conforms to Structure
• Loss on Drying (105°C / 4h): ≤ 0.5%
• Insoluble Matter: ≤ 0.2%
• Phosphate: 49.0 - 51.0%
• Fluoride: ≤ 10 ppm
• Arsenic: ≤ 1 ppm
• Lead: ≤ 1 ppm
• RNase: None Detected
• DNase: None Detected

*Custom sizes available upon request.

DOT Information: Non-regulated.
 

Electrophoresis Buffers
QuickSilver - TAE (Tris-acetate-EDTA)

TAE is ideal for separation of large DNA fragments in agarose gels and is used as running buffer and in gel preparation.

TAE is the buffer of choice when recovering DNA from gels and when downstream processing involves enzymatic reactions. Final concentration of a 1X working solution is 40mM Tris, 20mM acetic acid, 1mM EDTA.

QS1000 QuickSilver TAE Buffer, 1X, 100 packs

QuickSilver - TBE (Tris-borate-EDTA)

TBE is used in gel preparation and as a running buffer for electrophoresis of nucleic acids. TBE works best for resolution of smaller fragments (<2kb). Its higher buffering capacity makes it useful for longer runs. The final concentration of a 1X working solution is 89mM Tris, 89mM boric acid, 2mM EDTA.

QS1001 QuickSilver TBE Buffer, 1X, 50 packs

QuickSilver - Fast Running Buffer

QuickSilver Fast Running Buffer is a proprietary blend formulated for agarose gel electrophoresis at high voltage (300-350V). Its strong buffering capacity minimizes heat generation all allows for extremely fast separation of fragments smaller than 5kb. A typical 30-minute run can be performed in just 10 minutes using this buffer.

QS1002 QuickSilver Fast Running Buffer, 1X, 100 packs
 
 

Protein Electrophoresis Buffers

QuickSilver™ TGS (Tris-Glycine-SDS)

Tris-Glycine-SDS is one of the most common running buffers used for SDS-PAGE. A 1X solution contains 25mM Tris, 192mM Glycine and 0.1% SDS.

QS1100 QuickSilver Tris Glycine-SDS Buffer, 1X, 50 packs

QuickSilver™ Tris-MES-SDS

Small to medium molecular weight proteins can be resolved on SDS-PAGE gels using Tris-MES-SDS as a running buffer. Proteins resolve faster in gels using Tris-MES-SDS as a running buffer than those using Tris-MOPS-SDS. A 1X solution has a final concentration of 50mM Tris, 50mM MES, 0.1% SDS and 1mM EDTA.

QS1101 QuickSilver Tris-MES-SDS Buffer, 1X, 50 packs

QuickSilver™ Tris-MOPS-SDS

Tris-MOPS-SDS is ideal for use as a running buffer when resolving medium to large proteins with SDS-PAGE. Gels run with Tris-MOPS-SDS buffer run slower than those using Tris-MES-SDS buffer. The final concentration of a 1X working solution is 50mM Tris, 50mM MOPS, 0.1% SDS and 1mM EDTA.

QS1102 QuickSilver Tris-MOPS-SDS, 1X, 50 packs

Western Blotting, ELISA, Cell Culture, Chromatography

QuickSilver™ PBS and PBST

Because its osmolality and ion concentration closely mimic that of the human body, PBS is commonly used for cell culture applications, as well as HPLC and MS. PBST is a wash buffer and diluent for ELISA. The 1X PBS solution contains 137mN NaCl, 2.7mM KCl and 10mM phosphate. PBST is supplemented with 0.05% Tween-20.

QS1200 QuickSilver PBS Buffer, 1X, 100 packs

QS1201 QuickSilver PBST Buffer, 1X, 100 packs

QuickSilver™ TBS and TBST

TBS and TBST are used to maintain pH within a very narrow range for a variety of applications including western blotting and immunoassays. The final concentration of a 1X working solution is 137mM NaCl, 2.7mM KCl, 50mM Tris. In addition, 1X TBST contains 0.1% Tween-20.

QS1202 QuickSilver TBS buffer, 1X, 100 packs

QS1203 QuickSilver TBST buffer, 1X, 100 packs