Azure Protein-free Blot Blocking Buffer

Protein-free blocking solution optimized for fluorescent Immunoblots and Western blots

Does not contain proteins. Azure Protein-Free Blot Blocking Buffer can reduce background in Western blots where the primary antibodies tend to cross-react with protein blockers like BSA, casein or milk protein. For low-quality primary antibodies that may require protein-based blocking agents, BSA or non-fat dry milk can be dissolved directly in the Azure Protein-Free Blot Blocking Buffer and then used to dilute the antibody.

Best used with assays where animal-based product usage is prohibited. Serves as an excellent blocking reagent for chemiluminescent Western blotting.

• Provided as a 5x solution
• Size: 200mL

Azure Transfer Buffer

This proprietary transfer buffer provides improved detection of low-abundance and post-translationally modified proteins.

High-efficiency protein transfer and increased protein retention on the membrane add up to more sensitive Western blots.

• Provided as a 50x concentrated solution. Sufficient for 50-100 blots
• Size: 500mL

Features

• High performance—increased protein retention on the membrane and fast transfer times result in more sensitive detection of low-abundance and post-translationally modified proteins
• Efficient—enhanced transfer efficiency for proteins of all sizes
• Fast—transfer proteins in less than 20 minutes vs. several hours required with traditional transfer buffers
• Convenient—use your existing wet transfer electrophoresis apparatus

Citric Acid, Monohydrate 

• Appearance (Color): Colorless or White
• Appearance (Form): Crystals
• Purity: 99.0 - 102.0%
• Identification (FTIR): Conforms to Structure
• Water: 7.5 - 8.8% 
• Lead: ≤ 3 ppm
• Iron: ≤ 3 ppm
• Oxalate: To Pass Test
• Sulfate: ≤ 20 ppm
• Chloride: ≤ 10 ppm
• Readily Carbonizable Substances: Passes Test
• Insoluble Matter: ≤ 0.005%
• Residue After Ignition: ≤ 0.02%
• DNase: None Detected
• RNase: None Detected

*Custom sizes available upon request.

DOT Information: Non-regulated.
 

PR1MA™ Potassium Acetate

CAS Number: 127-08-2
Molecular Weight: 98.14
Chemical Formula: C₂H₃O₂K
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White crystalline powder
• Calcium (Ca): ≤ 0.005 %
• Chloride (Cl): ≤ 0.003 %
• Heavy Metals : ≤ 5 ppm
• Insolubles: ≤ 0.005 %
• Iron: ≤ 5 ppm
• Magnesium (Mg): ≤ 0.002 %
• pH (5% in water): 6.5 - 9.0
• Phosphate: ≤ 0.001 %
• Purity: ≥ 99.0 %
• Sodium (Na): ≤ 0.03 %
• Sulfate: ≤ 0.002 %
• Country of Origin: Report

PR1MA™ bis-TRIS [bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane]

Common Name: bis(2-Hydroxyethyl)aminotris (hydroxymethyl)methane
CAS Number: 6976-37-0
Molecular Weight: 209.2
Chemical Formula: C₈H₁₉NO₅
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White crystalline powder
• Assay: ≥ 99.0 %
• Identification (IR): Matches reference
• Melting Point: Report ^oC
• pH, 1.0M Aqueous solution @25ºC: 9.5 - 11.0
• pKa @ 20°C: 6.0 - 7.0
• Solubility: Complete and clear
• UV Absorbance @280nm, 1.0 M in H₂O: ≤ 0.40

PR1MA™  HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)]

Common Name: [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)]
CAS Number: 7365-45-9
Molecular Weight: 238.3
Chemical Formula: C₈H₁₈N₂O₄S
Solubility: Water
Storage Temp: Room Temperature

Zwitterionic N-substituted aminosulfonic acid buffer.

• pka (25°C): 7.5
• pH Range (Working): 6.8 - 8.2
• Heavy Metals: <0.001% Max

• Appearance: White Crystalline Powder
• Assay (Titration, dried basis): ≥ 99.5 %
• DNase, RNase, protease: Not Detected
• Endotoxin (0.2% Solution): ≤ 0.25 EU/ml
• Heavy Metals (as Pb): ≤ 0.001 %
• Identification (IR): Pass
• Iron (Fe): ≤ 0.0005 %
• Loss on Drying: ≤ 1.0 %
• pH (5% in water): 5.0 - 6.5
• pKa @ 20°C: 7.35 - 7.70
• Residue on Ignition: ≤ 0.1 %
• Solubility (1M Solution in Water ): Clear and Colorless Solution
• Sulfate: ≤ 0.05 %
• UV Absorbance (1M@260nm): ≤ 0.05
• UV Absorbance (1M@280nm): ≤ 0.05

PR1MA™ Potassium Phosphate, Dibasic, Anhydrous

CAS Number: 7758-11-4
Molecular Weight: 174.2
Chemical Formula: K₂HPO₄
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Powder
• Assay: ≥ 98.0 %
• Chloride: ≤ 0.003 %
• Heavy Metals : ≤ 5 ppm
• Insoluble Matter: ≤ 0.01 %
• Iron (Fe): ≤ 0.001 %
• Loss on Drying @150 ^oC: ≤ 1.0 %
• Nitrogen Compound: ≤ 0.001 %
• pH (5%): 8.5 - 9.6
• Sodium (Na): ≤ 0.05 %
• Sulfate: ≤ 0.005 %

Citric Acid, Anhydrous

• Appearance (Color): Colorless or White
• Appearance (Form): Crystals or Crystalline Powder
• Purity: ≥ 99.5%
• Identification (FTIR): Conforms to Structure
• Water: ≤ 0.3%
• Lead: ≤ 5 ppm
• Iron: ≤ 5 ppm
• Oxalate: ≤ 100 ppm
• Sulfate: ≤ 150 ppm
• Chloride: ≤ 50 ppm
• Barium: Passes Test
• Residue on Ignition: ≤ 0.05%
• Readily Carbonizable Substances: Passes Test
• Bacterial Endotoxins: ≤ 0.5 IU/mg
• DNase: None Detected
• RNase: None Detected 

*Custom sizes available upon request.

DOT Information: Non-regulated.   
 

PR1MA™ Potassium Phosphate, Monobasic, ACS

CAS Number: 7778-77-0
Molecular Weight: 136.1
Chemical Formula: KH2PO4
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White crystalline powder
• Odor: Odorless
• pH (5% Solution): 4.1 - 4.5
• Insoluble Matter: ≤ 0.01 %
• Loss on Drying: ≤ 0.2 %
• Chloride (Cl): ≤ 0.001 %
• Sulfate: ≤ 0.003 %
• Heavy Metals: ≤ 0.001 %
• Iron: ≤ 0.002 %
• Sodium (Na): ≤ 0.005 %
• Assay: ≥ 99.0 %
• Grade: ACS
• BSE/TSE Free: BSE/TSE Free
• Non-Animal Origin: Non-Animal Origin
• Country of Origin: Report

RunBlue SDS Running Buffer (NXB50500) a TEO-Tricine buffer system which provides a separation similar to the MOPS buffer used with BIS-TRIS precast gels. It provides enhanced separation of higher molecular weight proteins. The buffer can be for reduced and non-reduced samples.

RunBlue TGS Blot Buffer(NXB82600) has been specially formulated to provide optimal blot transfer of proteins from RunBlue Precast Gels. Supplied as a concentrate should be diluted by a factor of 10 for use with the RunBlue Dual Run and Blot system or a factor of 20 for use with other blotting methods.

PR1MA™ HEPPS [N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)] [EPPS]

Common Name: HEPPS [N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)] [EPPS]
CAS Number: 16052-06-5
Molecular Weight: 252.3
Chemical Formula: C₉H₂₀N₂O₄S
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Crystalline Powder
• Assay (Titration, dried basis): ≥ 99.0 %
• Loss on Drying: ≤ 1.0 %
• pH (1mol/L in Water @ 20 ^oC: 5.0 - 7.0
• Solubility (0.1M in water): Clear and Colorless

PR1MA™  TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl]

Common Name: Tris(hydroxymethyl) aminomethane hydrochloride
CAS Number: 1185-53-1
Molecular Weight: 157.6
Chemical Formula: C₄H₁₁NO₃ • HCl
Solubility: Water
Storage Temp: Room Temperature

Highly purified for use in molecular biology applications.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White crystalline
• Assay: 99.0 - 101.0 %
• pH (1% aqueous solution): 4.2 - 5.0
• Solubility (1M Solution in Water ) : Clear, colorless solution
• UV Absorbance @260nm, 1.0 M in H₂O: ≤ 0.06
• UV Absorbance @280nm, 1.0 M in H₂O: ≤ 0.05
• Identification: Conforms
• Heavy Metals : ≤ 5 ppm
• Grade: USP