EZ Pack™ Agarose Tablets

• Eliminates weighing
• Fast dissolving, just two minutes
• Environmentally friendly, free from organic solvents
• Consistent, reproducible gels
• Enhanced resolution and clarity 

Researchers trust Benchmark’s Agarose LE to meet their electrophoretic needs for various applications. Now, the same agarose is available in a convenient, no-mess tablet.

Agarose LE is refined in an advanced process that excludes the use of organic solvents. The result is a cleaner end product with significantly reduced environmental impact. The agarose can be used for analyses of nucleic acids from 150 bp to 25,000 bp, protein electrophoresis, and various blotting protocols.

The low EEO of the agarose promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO reduces band distortion (caused by counterflow) that can result from the presence of excessive sulfate-rich negative ions. The quick-dissolving EZ Pack™ tablet contains 0.5 g (500 mg) of Agarose LE, eliminating the hands-on time and inaccuracies normally associated with weighing. Use of the tablet is not only convenient and cleaner, but it also provides better consistency and reproducibility gel to gel.

 

Gel preparation is simple – add the desired number of tablets to the electrophoresis buffer, allow it to sit for two minutes, and then heat and pour as usual. The resulting gels are highly transparent, have exceptional thermal stability (ensuring safe and easy handling), and exhibit exceptionally low absorption of chemical staining agents.

EZ Pack™ Agarose Tablets are supplied in convenient blister packs for safe, clean dispensing.

 

Specifications
 

Gel Temperature	36°C ± 1.5°C (1.5%)
Melt Temperature	88°C ± 1.5°C (1.5%)
EEO (-mr)	< 0.13
Moisture Content	< 10%
Sulfate	< 0.2%
RNase/DNase	None Detected
Protease/Endonuclease	None Detected
Storage Conditions	Room Temperature
Gel Strength	> 1200 g / cm2 (1%) > 2500 g / cm2 (1.5%)

 

Buffer volume required to achieve desired gel strength
GEL %	0.8%	1.0%	1.2%	1.3%	1.5%	1.8%	2.0%
1 Tab	63 mL	50 mL	42 mL	38 mL	33 mL	28 mL	25 mL
2 Tabs	125 mL	100 mL	83 mL	77 mL	67 mL	56 mL	50 mL
3 Tabs	188 mL	150 mL	125 mL	115 mL	100 mL	83 mL	75 mL

Citric Acid, Monohydrate 

• Appearance (Color): Colorless or White
• Appearance (Form): Crystals
• Purity: 99.0 - 102.0%
• Identification (FTIR): Conforms to Structure
• Water: 7.5 - 8.8% 
• Lead: ≤ 3 ppm
• Iron: ≤ 3 ppm
• Oxalate: To Pass Test
• Sulfate: ≤ 20 ppm
• Chloride: ≤ 10 ppm
• Readily Carbonizable Substances: Passes Test
• Insoluble Matter: ≤ 0.005%
• Residue After Ignition: ≤ 0.02%
• DNase: None Detected
• RNase: None Detected

*Custom sizes available upon request.

 

DOT Information: Non-regulated.
 

This product has been discontinued.

Please visit the New PR1MA™ qMAX Green qPCR Mix with Blue Tracking Dye for more options!

 

 

PR1MA™ qMAX™ Gold

• Inert yellow dye helps reduce pipetting errors
• Room temperature stable for up to 30 days
• Compatible with fast cycling protocols
• Highly sensitive for low copy number templates
• Includes PR1MA Hot Start Taq Polymerase

Successful PCR requires careful control of many variables.  Pipetting errors, poor performing polymerases, dNTP concentrations, are just a few of the variables that can all contribute to reaction problems.  PR1MA has developed their new qMAX™ Gold to control these variables and help you achieve the best possible amplification performance. To reduce the chance of pipetting errors, qMAX™ Gold includes an inert yellow dye so small volumes are easy to visualize in PCR plates.  

qMAX™ Gold is a ready to use, 2X mastermix of PR1MA Hot Start Taq enzyme, dNTPs, a sensitive fluorescent intercalating dye, and optimized reaction buffer.  The Hot Start Taq allows reaction set up at room temperature while the temperature stable formulation guarantees optimal performance even if the mix is left at room temperature for extended periods.

Just add primers and DNA targets to the mix, and then proceed to amplification.  qMAX™ Gold is compatible with all real time thermal cyclers and exhibits high sensitivity with normal or fast 2-step cycling protocols.
 *Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.
 
 

This brand is being discontinued.

Need a great DNA Ladders at a great price?

Try our PR1MA™ SmartCheck DNA Ladders!

Bullseye 100bp DNA Ladder RTU (Ready-to-Use)

• A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 11 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis.
• The DNA includes fragments ranging from 100-1,500 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points.
• The approximate mass of DNA in each band is provided (0.5 ug a load) for approximating the mass of DNA in comparably intense samples of similar size.

Source:

• PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol and equilibrated to 10nM Tris-HCl (pH 8.0) and 10 mM EDTA.

Range: 100-1,500 bp (DL1); 250-10,000 bp (DL5)
Number of bands: 11 (DL1); 13 (DL5)
Concentration: 100 ug / mL
Package: 50 ug / 500 uL
Recommended Load: 5 uL / well
Contains orange G & xylene cyanol FF as tracking dyes. (DL1)
Contains bromophenol blue & xylene cyanol FF as tracking dyes. (DL5)

Storage:

• Store at 25°C for 6 months
• Store at 4°C for 12 months
• Store at -20°C for 24 months

Bullseye 100bp DNA Ladder

• Ready to use
• Contains 11 DNA bands: 100-1500bp.
• Clearly identifiable 500bp band as reference
• 500ng DNA/6 uL/loading
• Easy to load
• Stable at room temperature
• Supplied with 6x sample loading buffer

Bullseye 100bp DNA Ladder consists of 11 DNA fragments ranging in size from 100-1500 base pairs (bp). 6 uL will yield at least 30 ng DNA in any single band. The intensity of the 500bp band has been increased to serve as a reference for easy identification.

Size: 1200 uL
Storage: Store at -20°C.
Concentration: 500 ng/6 uL

Loading Buffer Composition:

• 10mM Tris-HCl
• 1mM EDTA (pH 8.0)
• 0.02% Bromophenol blue
• 0.02% Xylene cyanol
• 5% Glycerol

Usage: Add at least 6 uL Bullseye 100bp DNA Ladder directly to wells designated for markers. You may need more than 6 uL of ladder, depending on well size and level of intensity needed to visualize the bands.

Please give us a call for a sample.

Pfu 2x Master Mix 
Pfu DNA Polymerase 2x master mix is ready to use premix which contains Pfu DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer. It has been optimized for routine PCR applications. Pfu DNA Polymerase is a heat stable DNA polymerase which has 5' 3' DNA polymerase and 3' 5' exonuclease (proofreading) activities. Pfu DNA polymerase retains the high fidelity, sensitivity and processivity with an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures. Pfu 2x Master Mix product is supplied with the unique Intact Genomics 5x Magic Enhancer that enables efficient amplification of GC rich templates up to 84%.

Quality Control 
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Product Source
E. coli strain expressing a Pfu DNA Polymerase gene from Pyrococcus furiosus.

Contents & Storage

1. Pfu 2x master mix
2. 5x Magic Enhancer

Store all contents at -20 °C.

1x Master Mix Composition 
10 mM Tris-HCl pH 9.0, 50 mM KCl, 1.5 mM MgCl, 0.2 mM dNTPs, 5% Glycerol, 0.08% Igepal CA 630, 0.05% Tween-20, 100 Units/ml Pfu DNA Polymerase.

Protocol
1. Prepare a reaction mix according to the following table:
  

PCR reaction set up:
Template DNA	1-50 ng
Forward primer (5 µM)	1.0µl
Reverse primer (5 µM)	1.0µl
Pfu 2x master mix	10.0µl
5x Magic Enhancer (optional)	4.0µl
HO up to	20.0µl

2. Mix the reaction mixture thoroughly.
3. Program the thermal cycler according to the manufacturer's instructions.
4. A typical PCR cycling program is outlined in the following table.

 

PCR cycling conditions:
Steps	Temp.	Time	Cycles
Initial Denaturation	95 °C	3 min	1
Denaturation	95 °C	30 sec	25-40
Annealing	50-66 °C	30 sec
Extension	72 °C	1 min/kb
Final Extension	72 °C	5 min	1
Hold	4-12 °C	

5. Place the PCR tubes in the thermal cycler and start the cycling program.

6. Analyze 5 µl of PCR products by agarose gel electrophoresis. 

Pfu DNA Polymerase 
Pfu DNA polymerase is a heat stable DNA polymerase which has 5' 3' DNA polymerase and 3' 5' exonuclease (proofreading) activities. Pfu DNA polymerase retains the high fidelity, sensitivity and processivity with an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures (1). This product is supplied with the unique Intact Genomics 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM, and 5X Magic Enhancer that enables efficient amplification of GC rich templates up to 84%. The physical purity of this enzyme is 98% as assessed by SDS-PAGE with Coomassie® blue staining.

Quality Control 
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Product Source
E. coli strain expressing a Pfu DNA Polymerase gene from Pyrococcus furiosus.

Contents & Storage

1. Pfu DNA Polymerase
2. 10x PCR Buffer with Mg²+
3. 5x Magic Enhancer
4. 10 mM dNTP (Cat. # 3312d, 3314d only)

Store all contents at -20 °C.

Storage Buffer 
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25 °C.

10X PCR Buffer with Mg2+ 
100 mM Tris-HCl pH 9.0, 15 mM MgCl, 100 mM KCl, 80 mM (NH4)2SO4, 0.5% Igepal CA 630

Unit Definition 
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72 °C.

Protocol
1. Thaw 10x PCR Buffer, dNTP mix, primer solutions, 5x Magic Enhancer (if required) and mix thoroughly before use. 
2. Prepare a reaction mix according to the following table: The reaction mix typically contains all the components needed for PCR except the template DNA.

 

PCR reaction set up:
Template DNA	xµl (0.01-0.5µMg)
10x PCR Buffer	10.0µl
dNTP (10 mM)	2.0µl
Forward Primer	xµl (0.1- 0.5µMM)
Reverse Primer	xµl (0.1- 0.5µMM)
5x Magic Enhancer (optional)	20µl
Pfu DNA Polymerase (5 U/µl)	0.5µl
H2O up to	100.0µl

3. Mix the reaction mixture thoroughly.
4. Add template DNA to the individual PCR tubes containing the reaction mixture. 
5. Program the thermal cycler according to the manufacturerâs instructions. A typical PCR cycling program is outlined in the following table.

PCR cycling conditions:
Steps	Temp.	Time	Cycles
Initial denaturation	95 °C	3-5 min	1
Denaturation	94 °C	30-60 sec	25-35
Annealing	52-66 °C	30-60 sec
Extension	72-74 °C	1-2 min
Final extension	72-74 °C	10 min	1
Hold	4-12 °C	â

6. Place the PCR tubes in the thermal cycler and start the cycling program.

This product is no longer available, please contact us for other options.
 

Looking for other alternatives?

Visit our PCR Reagents page for more options! 

Cas9 Nuclease 
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by using CRISPR RNAs (crRNAs) to guide the silencing of invading nucleic acids. The CRISPR system consists of a short non-coding guide RNA (sgRNA) made up of a target complementary CRISPR RNA (crRNA) and an auxiliary transactivating crRNA (tracrRNA). The sgRNA guides the Cas9 endonuclease to a specific genomic locus via base pairing between the crRNA sequence and the target sequence, and cleaves the DNA to create a double-strand break. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence

Intact Genomics Cas9 Nuclease is the purified recombinant Streptococcus pyogenes Cas9 enzyme containing a nuclear localization signal (NLS) at the C-terminal for targeting to the nucleus. This enzyme is designed to perform CRISPR/Cas9-mediated genome editing. The physical purity of this enzyme is â¥98% as assessed by SDS-PAGE with Coomassie® blue staining.

Quality Control 
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Cas9 nuclease is free from detectable RNase, Endonuclease (nicking) and non-specific DNase activities.

Product Source 
E. coli BL21 (DE3) strain expressing a Cas9 gene from Streptococcus pyogenes with an N-terminal 6xHis tag and C-terminal SV40 nuclear localization signal (NLS).

Contents & Storage

1. Cas9 Nuclease
2. 10x Cas9 Nuclease Reaction Buffer

Store Cas9 Nuclease and Buffer at -20 °C

Storage Buffer 
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25 °C

1x Cas9 Reaction Buffer 
20 mM HEPES, 100 mM NaCl, 5 mM MgCl, 0.1 mM EDTA, pH 6.5 @ 25 °C

Functional Testing
Cas9 Nuclease functional testing was done by in vitro DNA cleavage assay with the following protocol which gives more than 95% digestion of the substrate DNA as determined by agarose gel electrophoresis.
1) Set up 30 µl reaction in a microcentrifuge tube on ice with the following combinations.

Target DNA	x µl (100ng)
sgRNA	x µl (4000ng)
10x Cas9 Reaction Buffer	3.0 µl
Cas9 Nuclease	1.0 µl (160ng)
Add H2O up to	30.0 µl

  

2) Gently mix the reaction mixture and centrifuge briefly.
3) Incubate at 37 °C for 60 min. 
4) Add 1 µl RNase (4 mg/ml) 
5) Incubate at 37 °C for 20 min.
6) Run 0.7 to1% agarose TBE gel.

Bullseye PREMIUM HS-Taq DNA Polymerase Master Mix Blue

HS Taq Polymerase Master Mix BLUE is a ready to use 2 X master mix. Simply add primers, template and water to successfully carry out primer extensions. The master mix is available in 100, 500, 1000, and 2500 reaction sizes and is available with two different buffer options (HS Buffer 1 and HS Buffer 2)

HS Taq Polymerase is a modified form of Bullseye Taq, activated by heat treatment. This results in higher specificity and greater yields when compared to standard Taq. The added benefit of the HS Master Mix Blue is that it can be loaded directly onto the gel as there is no need to use separate loading dyes for subsequent electrophoresis and visualization. This cuts down on the chance of contaminating the component stocks and leads to better reproducibility.

Composition of 2 X HS Master Mix Blue with Buffer 1
Tris-HCl, pH 8.5, (NH4)2SO4,3 mM, MgCl2, .2 % Tween 20, .4 mM dNTPs, .2 units/uL HS Taq Polymerase, Inert Blue Dye, Stabilizer

Composition of 2 X HS Master Mix Blue with Buffer 2
Tris-Hcl, pH 8.5, Balanced KCl/(NH4)2SO4,3 mM, MgCl2, .2 % Tween 20, .4 mM dNTPD, .2 units/uL HS Taq Polymerase, Inert Blue Dye, Stabilizer

Bullseye Taq DNA Polymerase

Ideal for General PCR, Genomic analysis and TA cloning!

Bullseye Taq DNA Polymerase is the most popular thermostable enzyme used in DNA amplification experiments. This high performance Taq DNA polymerase is specifically purified to produce excellent yields with little or no background. Its outstanding activity and unique thermal DNA amplification properties make it one of the best-value DNA polymerases available.

• Robust performance
• Leaves 3' A overhang
• Stable at all storage temperatures
• Ideal for general PCR, genomic analysis and TA cloning

Item #	Description	Quantity	Rxn
BETAQ-1000	Taq DNA Polymerase	1 x 200 uL	1000U
BETAQ-5000	Taq DNA Polymerase	5 x 200 uL	5000U

Storage: -20°C

Quality Control
Taq DNA Polymerase is highly purified, free of contaminating endonucleases, exonucleases and nicking activity. For endonuclease assay, 1 ug of Lamda/Hind III DNA is incubated with 20 units of enzyme in assay buffer at 75oC for 16 hours with no visible contaminating activity observed. Also, every lot is tested for its performance consistency.

Unit Definition
One unit incorporates 10n moles of 4 radioactive labeled dNTPs into acid-insoluble material in 30 minutes at 74°C.

Storage Buffer
5 units / uL in 50 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol, 0.5% TritonX-100, and 0.5% NP-40.
10x Reaction Buffer 100 mM KCl, 100 mM Tris HCl (pH9.0), 80 mM (NH4)2SO4, and 1.0% Triton X-100. (Mg++free): is optimized for use with 200 uM dNTPs.

Magnesium Chloride
25 mM MgCl2: In general, 1.5 mM MgCl2 is recommended; this may vary with different conditions and primer sets.
 
*For laboratory research only. Not for clinical applications. 

Contact us for other options!

This brand is being discontinued, please contact us for other options.
 

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Bullseye PREMIUM GC Rich Optimized DNA Polymerases and Kits

Bullseye GC HS 2x Master Mix I is an all-in-one 2x master mix containing HS DNA polymerase, GC Buffer I, enhancer, dNTPs and MgCl2. Simply mix GC HS 2x Master Mix I with primers, template and water and you are ready to carry out successful primer extensions. HS DNA Polymerase is a modified form of Bullseye Taq DNA polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity, increased sensitivity and greater yields when compared to standard DNA polymerases.

Key Features

• For amplification of DNA targets with high GC content
  
• Convenient reaction set-up at room temperature
  
• High specificity, sensitivity and product yield
  
• Detection of low abundance targets
  
• Diminished formation of non-specific product

  Composition of GC HS 2x Master Mix I
  

• HS DNA Polymerase
  
• Optimized buffer components, 3.0 mM MgCl
  
• dNTPs
  
• Enhancer