PR1MA™ Rapid Coomassie Stain

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PR1MA™ Malt Extract Agar, Powder

CAS Number: 8002-48-0
Solubility: Water with Heat
Storage Temp: Room Temperature

Malt Extract Agar is used for the isolation, cultivation, and maintenance of yeasts and molds. Formulation is similar to Malt Extract Broth with the addition of agar and glycerol. This broth medium was recommended by Thom and Church for the cultivation of fungi. Its low acidity inhibits the growth of most bacteria. The high carbohydrate content enhances the rapid growth of fungi and yeasts.

Dissolve 33.6 grams in 1 liter of distilled water. Sterilize by autoclaving for 15 minutes.

Ingredients:

• Maltose: 12.75 g/L
• Dextrin: 2.75 g/L
• Glycerol: 2.35 g/L
• Peptone: 0.78 g/L
• Agar: 15.0 g/L
• Final pH (25°C): 4.6 ± 0.2

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous with soft lumps, free flowing, light beige
• PPM Appearance: Trace to moderate haze, off white to pale yellow
• pH(3.36% Solution @ 25 ^oC: 4.5 - 4.9
• Microorganism (Aspergillus niger ATCC 16404): Growth
• Microorganism (Candida albicans ATCC 10231): Growth
• Microorganism (Penicillium Roquefortii ATCC 10110): Growth
• Microorganism (Saccharomyces cerevisiae ATCC 9763): Growth
• Microorganisms (Microsporum Audouinii ATCC 42558): Growth
• Microorganisms (Microsporum Canis ATCC 36299): Growth
• Microorganism (Trichophyton Mentagrophytes ATCC 9533): Growth

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PR1MA™ 2XYT Broth, Capsules

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temp: Room Temperature

Nutritionally enriched medium for the growth of recombinant strains of E. Coli and in the preparation and transformation of competent cells.

2XYT Broth packaged in convenient ready to use capsules. Each capsule contains 15.5 g 2XYT Broth.

Simply add 2 capsules to one liter distilled water and heat until completely dissolved. Sterilize by autoclaving at 121°C for 15 minutes. Final pH at 25°C: 6.8-7.2.

Ingredients:

• Digest Peptone: 16 g/l
• Yeast Extract: 10 g/l
• Sodium Chloride:5 g/l

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsule (Cream to beige free flowing powder)
• DCM Appearance: Capsule (Homogeneous, free flowing, beige)
• PPM Appearance: Capsule (Clear, no to light ppt, yellow)
• pH (3.1 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Casein Peptone: 16.0 g/L
• Yeast Extract: 10.0 g/L
• Sodium Chloride: 5.0 g/l
• Description: Add 2 capsules 2XYT broth in 1 liter purified water until evenly dispersed. Boil for 1 minute with stirring to completely dissolve. Distribute and autoclave at 121^oC for 15 minutes.

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PR1MA™ 2XYT Broth, Powder

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temp: Room Temperature

Nutritionally enriched medium for the growth of recombinant strains of E. Coli and in the preparation and transformation of competent cells.

Dissolve 31g in one liter distilled water. Heat solution to boiling with continuous stirring. Sterilize by autoclaving at 121°C for 15 minutes. Final ph @ 25°C: 6.8-7.2.

Ingredients:

• Digest Peptone: 16 g/l
• Yeast Extract: 10 g/l
• Sodium Chloride: 5 g/l

Research or further manufacturing use only, not for food or drug use.

• Appearance: Cream to beige free flowing powder
• DCM Appearance: Homogeneous free flowing, beige
• PPM Appearance: Clear,no to light ppt,yellow
• pH (3.1 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

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PR1MA™ Luria Broth Granulated [Miller's LB Broth]

Common Name: Miller's LB Broth
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temp: Room Temperature

For propagation and maintenance of E. Coli. High Salt Formula. Molecular Biology Grade.

Dissolve 25 grams in 1 liter distilled water and adjust the pH to 7.2. Heat and frequently agitate to completely dissolve. Sterilize by autoclaving for 15 minutes. Material specially granulated to reduce dust and increase solubility rate.

Ingredients:

• Tryptone:10 g/L
• Sodium Chloride: 10 g/L
• Yeast Extract: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Cream to beige granular powder
• DCM Appearance: Homogeneous, free flowing beige to yellow-beige
• PPM Appearance: Clear, yellow to amber no precipitate (ppt)
• Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
• pH (2.5% H₂O @25 °C): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Casein Peptone: 10 g/L
• Sodium Chloride: 10 g/L
• Yeast Extract: 5 g/L

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PR1MA™ Yeast Extract, Granulated

CAS Number: 8013-01-2
Solubility: Water
Storage Temp: Room Temperature

Dried yeast autolysate with a high content of amino nitrogen and water soluble B complex vitamins. Material is specially granulated to reduce dust and increase solubility rate.

Suitable for Microbiology Applications.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Light Beige Powder
• Amino Nitrogen Content: ≥ 3.5 %
• Dry Matter: ≥ 94 %
• Moisture : ≤ 6.0 %
• pH (2.0% Solution @ 25 ^oC): 6.5 - 7.5
• Sodium Chloride: ≤ 0.5 %
• Total Nitrogen Content: ≥ 8.0 %
• Non Animal Origin: The product does not come in contact with materials of animal origin.

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PR1MA™ Luria Agar Powder [Miller's LB Agar]

Common Name: Miller's LB Agar
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature

For propagation and maintenance of E. Coli. High Salt Formula. Molecular Biology Grade.

• Dissolve 40 grams in 1 liter distilled water
• Adjust pH to 7.2
• Sterilize by autoclaving for 15 minutes

Ingredients:

• Peptone: 10g/L
• Yeast: 5 g/L
• Sodium Chloride: 10g/L
• Agar: 15g/L

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous Free Flowing, Light Beige
• PPM Appearance: Clear, no to light ppt, light yellow
• pH (4% solution at 25°C): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

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PR1MA™ 2XYT Broth, Granulated

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temp: Room Temperature

Nutritionally enriched medium for the growth of recombinant strains of E. Coli and in the preparation and transformation of competent cells.

Material is specially granulated to reduce dust and increase solubility rate.

Dissolve 31g in one liter distilled water. Heat solution to boiling with continuous stirring. Sterilize by autoclaving at 121°C for 15 minutes. Final pH@ 25°C: 6.8-7.2.

Ingredients:

• Digest Peptone: 16 g/l
• Yeast Extract: 10 g/l
• Sodium Chloride: 5 g/l

Research or further manufacturing use only, not for food or drug use.

• Appearance: Cream to beige free flowing granulated
• DCM Appearance: Homogeneous free flowing, beige
• PPM Appearance: Clear,no to light ppt,yellow
• pH (3.1 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

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PR1MA™ Sodium Chloride, Powder

CAS Number: 7647-14-5
Molecular Weight: 58.4
Chemical Formula: NaCl
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

• Appearance: Fine White Granular Powder
• Acidity or Alkalinity: Pass Test
• Appearance of Solution: Clear, Colorless Solution
• Arsenic : ≤ 1 µg/g
• Assay: 99.0 - 100.5 %
• Barium: Pass Test
• Bromides: ≤ 0.010 %
• Ferrocyanide: Pass Test
• Heavy Metals : ≤ 5 ppm
• Identification (Chloride): Pass Test
• Identification (Sodium): Pass Test
• Loss on Drying: ≤ 0.5 %
• Magnesium & Alkaline Earth Metals:  0.01 %
• Iron(Fe): ≤ 2 µg/g
• Nitrites: ≤ 0.01
• Phosphates : ≤ 0.0025 %
• Sulfate: ≤ 0.020 %

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PR1MA™ Protein Markers

PR1MA™ All Blue, Regular Range Protein Markers

PR1MA™ All Blue, Regular Range Protein Markers are blue protein standards with 10 or 11 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa, in the presence of Tris- Glycine buffer) are enhanced in intensity.

Loading Volume

• 3 μL or 5 μL per loading for clear visualization during electrophoresis on a 15-well or 10-well mini-gel
• 1.5~2.5 μL per well for general Western blotting transfer

The markers are supplied at a concentration of approximately 0.1~0.5 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol)

PR1MA™ Tricolor Protein Markers

PR1MA™ Tricolor Protein Markers are three-color protein standards with 10, 12, or 13 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine buffer (3.5 kDa to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands, one green and one red band (at 25 kDa and 75 kDa, respectively, in the presence of Tris-Glycine buffer).

Loading Volume

• 3 μL or 5 μL per loading for clear visualization during electrophoresis on a 15-well or 10-well mini-gel.
• 1.5~2.5 μL per well for general Western blotting transfer.

The markers are supplied at a concentration of approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

Specifications

Item#	Color	Bands	Range
PR5010-BL	Blue	10	9-180 kDA
PR5011-BL	Blue	11	9-180 kDA
PR5010-TC	Tri-Color	10	9-180 kDA
PR5012-TC	Tri-Color	12	9-245 kDA
PR5013-TC	Tri-Color	13	9-245 kDA

 

PR1MA™ Glycerol

CAS Number: 56-81-5
Molecular Weight: 92.1
Chemical Formula: C₃H₈O₃
Solubility: Water
Storage Temp: Room Temperature

Used in sample preparation and gel formation for polyacrylamide gel electrophoresis. Also used in casting gradient gels, as a protein stabilizer and storage buffer component.

Research or further manufacturing use only, not for food or drug use.

• Appearance: Clear and Colorless Liquid
• Chloride (Cl): ≤ 0.001 %
• Chlorinated Compounds: ≤ 0.003 %
• Color, APHA: ≤ 10.0
• Fatty Acids & Esters (USP): ≤ 1.0 (ml 0.5 N NaOH) %
• Glycerine: ≥ 99.7 %
• Heavy Metals: ≤ 5.0 ppm
• Identification C (GC): Pass
• Limit of Diethylene Glycol & RC: Pass
• Purity (USP): 99.0 - 101.0 %
• Residue on Ignition: ≤ 0.01 %
• Specific Gravity: ≥ 1.259
• Water: ≤ 5.0 %
• Date of Manufacture: Report

PR1MA™ Agar, Phyto, Powder

CAS Number: 9002-18-0
Solubility: Water
Storage Temp: Room Temperature

Specially selected and tested agar for use in plant tissue culture. High gel strength.

Concentration of 5.0 g/l obtains a solid gel strength: (1600 g/cm2).

Research or further manufacturing use only, not for food or drug use.

• Appearance: Pale Yellow Powder
• Gelling Point: 34 - 36 °C
• Gel Strength : 950 - 1050 g/cm²
• Identification: Conforms
• Loss on Drying: ≤ 18 %
• Melting Point: 85 - 95 ^oC
• Nephelos: ≤ 40 NTU
• pH: 6.0 - 7.5
• Total Ash: ≤ 3.5 %
• Gelatin: Absent
• Starch: Absent
• Total Plate Count: ≤ 1000 cfu/g