Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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PR1MA Yeast Extract, Granulated

CAS Number: 8013-01-2
Solubility: Water
Storage Temp: Room Temperature

Dried yeast autolysate with a high content of amino nitrogen and water soluble B complex vitamins. Material is specially granulated to reduce dust and increase solubility rate.

Suitable for Microbiology Applications.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Light Beige Powder
  • Amino Nitrogen Content:  3.5 %
  • Dry Matter:  94 %
  • Moisture :  6.0 %
  • pH (2.0% Solution @ 25 oC): 6.5 - 7.5
  • Sodium Chloride:  0.5 %
  • Total Nitrogen Content:  8.0 %
  • Non Animal Origin: The product does not come in contact with materials of animal origin.

Proudly made in the USA! Click here for more made in America products!
 
 

[Miller's LB Agar]

PR1MA Luria Agar Powder [Miller's LB Agar]

Common Name: Miller's LB Agar
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature

For propagation and maintenance of E. Coli. High Salt Formula. Molecular Biology Grade.

  • Dissolve 40 grams in 1 liter distilled water
  • Adjust pH to 7.2
  • Sterilize by autoclaving for 15 minutes

Ingredients:

  • Peptone: 10g/L
  • Yeast: 5 g/L
  • Sodium Chloride: 10g/L
  • Agar: 15g/L

Research or further manufacturing use only, not for food or drug use.

  • DCM Appearance: Homogeneous Free Flowing, Light Beige
  • PPM Appearance: Clear, no to light ppt, light yellow
  • pH (4% solution at 25°C): 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Fair to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent

Proudly made in the USA! Click here for more made in America products!
 
 

PR1MA 2XYT Broth, Granulated

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5
Solubility: Water
Storage Temp: Room Temperature

Nutritionally enriched medium for the growth of recombinant strains of E. Coli and in the preparation and transformation of competent cells.

Material is specially granulated to reduce dust and increase solubility rate.

Dissolve 31g in one liter distilled water. Heat solution to boiling with continuous stirring. Sterilize by autoclaving at 121°C for 15 minutes. Final pH@ 25°C: 6.8-7.2.

Ingredients:

  • Digest Peptone: 16 g/l
  • Yeast Extract: 10 g/l
  • Sodium Chloride: 5 g/l

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Cream to beige free flowing granulated
  • DCM Appearance: Homogeneous free flowing, beige
  • PPM Appearance: Clear,no to light ppt,yellow
  • pH (3.1 % Solution @25 oC): 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Good to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent

Proudly made in the USA! Click here for more made in America products!
 
 

PR1MA Tryptone, Powder

CAS Number: 91079-40-2
Solubility: Water
Storage Temp: Room Temperature

Pancreatic digest of casein used to supplement media in cultivation of anaerobic bacteria.

pH (1% solution): 7.3.

Ingredients:

  • Molecular Biology GradeTotal Nitrogen (TN): ~12.7%
  • Amino Nitrogen (AN): ~3.7%
  • AN/TN Ratio: 0.26
  • Sodium Chloride: ~0.4%
  • Tryptophan: ~1.0

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Cream to beige free flowing powder
  • Amino Nitrogen:  4.0 %
  • Ash:  15 %
  • Loss on Drying:  5.0 %
  • Nitrites: Negative
  • pH (2% water) @25°C: 6.5 - 7.5
  • Total Nitrogen:  10 %
  • Undigested Protein: Negative
  • Broth Formulations (Growth Supporting Properties): Satisfactory
  • Peptone Agar (Growth Supporting Properties): Satisfactory
  • Proteoses: Positive
  • Salmonella: Negative/25g
  • Standard Plate Count :  1000 cfu/g
  • Coliform: Negative
  • E.Coli: Negative
  • Molds and Yeasts:  100 cfu/g
  • Raw Material Species: Bovine
  • Enzyme Species: Porcine
  • BSE/TSE Free: BSE/TSE Free
  • Country of Origin : Report

Proudly made in the USA! Click here for more made in America products!
 
 

PR1MA Peptone, Granulated

CAS Number: 73049-73-7
Solubility: Water
Storage Temp: Room Temperature

Mixtures of peptides and free amino acids from peptic digest of animal tissue.

  • Amino Nitrogen (AN): 2.5-3.5%
  • Loss on Drying: <6.0%

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Light Beige Granular Powder
  • Amino Nitrogen Content:  3.4 %
  • Appearance of Solution: Clear and yellow-beige
  • Ash:  15.0 %
  • Loss on Drying:  5.0 %
  • pH (2.0% Solution @ 25 oC): 6.5 - 7.5
  • Total Nitrogen Content:  10.0 %

Proudly made in the USA! Click here for more made in America products!
 
 

PR1MA qMAX Green qPCR Mix with Blue Tracking Dye

  • Superior sensitivity and fast cycling with exceptional results
  • Ideal for low copy number templates
  • Early Ct values and detection across a broad dynamic range
  • Ready to use 2X Master Mix
  • Includes PR1MA Hot Start Taq Polymerase for greater specificity and accuracy 

Supplied as a ready-to-use 2X master mix, PR1MA qMax Green has been engineered for high sensitivity, fast cycling, and excellent reproducibility. PR1MA Hot Start Polymerase provides accurate PCR of a variety of templates including low copy number and difficult sequences, while the proprietary qMax Green intercalating dye exhibits higher fluorescence and lower PCR inhibition than other popular green dyes.

 

These two components are supported by a specially formulated buffer with an exacting combination of salts, PCR enhancers, stabilizers, and pH that results in earlier Ct values and a high specificity across a broad dynamic range.


*Please note these products ship on dry ice. Appropriate shipping charges apply unless otherwise noted on a quote.

Proudly made in the USA! Click here for more made in America products!
 
 

PR1MA Sodium Chloride, Powder

CAS Number: 7647-14-5
Molecular Weight: 58.4
Chemical Formula: NaCl
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Fine White Granular Powder
  • Acidity or Alkalinity: Pass Test
  • Appearance of Solution: Clear, Colorless Solution
  • Arsenic :  1 µg/g
  • Assay: 99.0 - 100.5 %
  • Barium: Pass Test
  • Bromides:  0.010 %
  • Ferrocyanide: Pass Test
  • Heavy Metals :  5 ppm
  • Identification (Chloride): Pass Test
  • Identification (Sodium): Pass Test
  • Loss on Drying:  0.5 %
  • Magnesium & Alkaline Earth Metals:  0.01 %
  • Iron(Fe):  2 µg/g
  • Nitrites:  0.01
  • Phosphates :  0.0025 %
  • Sulfate:  0.020 %

Proudly made in the USA! Click here for more made in America products!
 
 


PR1MA Glycerol

CAS Number: 56-81-5
Molecular Weight: 92.1
Chemical Formula: C3H8O3
Solubility: Water
Storage Temp: Room Temperature

Used in sample preparation and gel formation for polyacrylamide gel electrophoresis. Also used in casting gradient gels, as a protein stabilizer and storage buffer component.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Clear and Colorless Liquid
  • Chloride (Cl):  0.001 %
  • Chlorinated Compounds:  0.003 %
  • Color, APHA:  10.0
  • Fatty Acids & Esters (USP):  1.0 (ml 0.5 N NaOH) %
  • Glycerine:  99.7 %
  • Heavy Metals:  5.0 ppm
  • Identification C (GC): Pass
  • Limit of Diethylene Glycol & RC: Pass
  • Purity (USP): 99.0 - 101.0 %
  • Residue on Ignition:  0.01 %
  • Specific Gravity:  1.259
  • Water:  5.0 %
  • Date of Manufacture: Report

Proudly made in the USA! Click here for more made in America products!
 
 

[Brain Heart Infusion Broth]

PR1MA  BHI Broth [Brain Heart Infusion Broth], Powder

Common Name: Brain Heart Infusion Broth
Solubility: Water
Storage Temp: Room Temperature

Recommended for the cultivation of rare microorganisms. Adding 0.1% Agar will enhance the growth of anaerobic organisms. Store at room temperature and protect from moisture and light.

Ingredients:
  • Brain Heart Infusion from solids: 17.5 g/l
  • Pancreatic Digest of Gelatin: 10.0 g/l
  • Dextrose: 2.0 g/l
  • Sodium Chloride: 5.0 g/l
  • Disodium Phosphate: 2.5 g/l
Research or further manufacturing use only, not for food or drug use.
  • DCM Appearance: Homogeneous free flowing, beige
  • PPM Appearance: Light to Medium Amber/Clear
  • pH (3.7% H2O @25 oC): 7.2 - 7.6
  • Microorganism (Aspergillus niger ATCC 16404): Poor to Very Good
  • Microorganism (Candida albicans ATCC 10231): Good to Excellent
  • Microorganism (Enterococcus faecalis ATCC 29212): Good to Excellent
  • Microorganism (Escherichia Coli ATCC 25922): Very Good to Excellent
  • Microorganism (Neisseria meningitides ATCC 13090): Fair to Good
  • Microorganism -Pseudomonas Aeruginosa ATCC 27853: Good to Excellent
  • Microorganism (Saccharomyces cerevisiae ATCC 9763): Good to Excellent
  • Microorganism (Staphylcoccus Aureus ATCC 25923): Very Good to Excellent
  • Microorganism (Streptococcus pneumoniae ATCC 6305): Good to Excellent
  • Microorganism (Streptococcus pyogenes ATCC 19615): Good to Excellent
  • Raw Material Species: Bovine
  • Enzyme Species: Porcine

Proudly made in the USA! Click here for more made in America products!
 
 


PR1MA Gelrite

CAS Number: 71010-52-1
Solubility: Water
Storage Temp: Room Temperature

Naturally derived gelling polymer that can be used in place of agar. Gelrite forms clear, rigid gels at approximately half the use level of agar in presence of soluble salts like Mg2+ and Ca2+.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Solid
  • Gel Strength: 400 - 700 g/cm2
  • Loss on Drying:  15.0 %
  • Particle Size 42 Mesh (355 µm):  95 %
  • Transmittance:  80.0 %

 

Proudly made in the USA! Click here for more made in America products!
 
 

PR1MA Agar, Phyto, Powder

CAS Number: 9002-18-0
Solubility: Water
Storage Temp: Room Temperature

Specially selected and tested agar for use in plant tissue culture. High gel strength.

Concentration of 5.0 g/l obtains a solid gel strength: (1600 g/cm2).

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Pale Yellow Powder
  • Gelling Point: 34 - 36 °C
  • Gel Strength : 950 - 1050 g/cm2
  • Identification: Conforms
  • Loss on Drying:  18 %
  • Melting Point: 85 - 95 oC
  • Nephelos:  40 NTU
  • pH: 6.0 - 7.5
  • Total Ash:  3.5 %
  • Gelatin: Absent
  • Starch: Absent
  • Total Plate Count:  1000 cfu/g

Proudly made in the USA! Click here for more made in America products!
 

[Casein acid hydrolysate]


PR1MA Casamino Acids [Casein acid hydrolysate]

Common Name: Casein acid hydrolysate
CAS Number: 91079-40-2
Solubility: Water
Storage Temp: Room Temperature

Casein Acid Hydrolysate is a hydrochloric acid digest of casein that contains small amounts of cystine and Tryptophan. All vitamins have been eliminated by the acidification treatment. Provides a source of amino acids as nutrients for growing various organisms tolerant of high salt conditions.

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Homogeneous, Free Flowing Powder
  • Color: Light Tan Yellow
  • Amino Nitrogen:  4.0 %
  • Moisture :  6.0 %
  • pH: 6.0 - 7.5
  • Protein: Report %
  • Solution (2%): Clear Light Yellow
  • Total Nitrogen: > 7.5 %
  • Coliforms: < 10 cfu/g
  • Standard Plate Count : < 5000 cfu/g
  • Thermophile Count: Report cfu/g
  • Source: Bovine

Proudly made in the USA! Click here for more made in America products!
 
 

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