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MIDSCI™ is Your Trusted Source for High-Quality Lab Chemicals and Reagents

Looking for reliable chemicals to power your research? MIDSCI™ delivers a full range of high-purity reagents, solvents, and buffers designed for life science, pharmaceutical, microbiology, and organic chemistry workflows. From essential acids and bases to specialized biochemicals, growth media, and DNA kits, our inventory ensures precision, consistency, and compliance, backed by Certificates of Analysis (COAs) and Safety Data Sheets (SDS).

Explore PR1MA™ Chemicals, our premium line of USP- and ACS-grade chemicals

Perfect for molecular biology, plant science, and cellular research, PR1MA™ products offer full traceability and documentation for your peace of mind.

Shop now for high-quality chemicals or contact our experts for personalized recommendations!

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PR1MA™ Tris-Acetate

Molecular Weight: 181.19
Chemical Formula: C14H11NO3C2H4O2
Solubility: Water
Storage Temp: Room Temperature

Moisture: <0.3%
pH (0.1M, H2O) @25°C: 6.0-7.0%
Heavy Metals: 0.001%

Shipping Weight: 0.3 lbs

Shipping Dimensions: 3.50 x 3.75 x 3.50
  • Appearance: White crystalline powder
  • Heavy Metals: ≤ 0.001 %
  • Moisture: ≤ 0.3 %
  • pH (0.1M Solution in water): 6.0 - 7.0
  • Purity: ≥ 98.0 %
  • Residue on Ignition: ≤ 0.1 %
  • BSE/TSE Free: BSE/TSE Free
  • Country of Origin: Report

  

Item#:
ASTRISACETATE
Thallos AM
 
Best in class, green fluorescent, thallium (Tl+) indicator, membrane permeable. Works with potassium (K+), sodium (Na+), and monovalent cation channels, and transporters.
 
Thallos is a green fluorescent, intracellular thallium (Tl+) indicator, and has been the gold standard for fluorescence-based potassium (K+) channel HTS for nearly 2 decades. Thallos also delivers outstanding results for a wide variety of monovalent cation (sodium – Na+) channels, transporters, and GPCRs.
Item#:
ASTHALLOSAM
 
THALLOS AM PROTOCOL
Thallos AM Safety Data Sheet
Thallos AM Product Specifications
Discontinued, Contact us for more options!

Western Blot 2.0 with PURITY™


The PURITY™ detection system revolutionizes Western Blotting in three steps.
 

Blocking buffer and secondary antibody are already components of the PURITY™ Reagent. And the washing buffer is also included. The only thing to add is your specific primary antibody.


Benefits:

  • 1 simple protocol which makes almost all optimization unnecessary.
  • Increased sensitivity and reproducibility of blots.
  • Dramatically reduces the number of steps and time required for Western blots.
  • Drastically decreases background phenomena.
  • Highly reliable.
Item#:
ASWESTBLOTACC
 
Western Blot 2.0 with PURITY

PR1MA™  Sodium Phosphate Dibasic, Anhydrous

CAS Number: 7558-79-4
Molecular Weight: 142.0
Chemical Formula: Na2HPO4
Solubility: Water
Storage Temp: Room Temperature

Research or further manufacturing use only, not for food or drug use.

  • Appearance: White Crystal
  • Assay: ≥ 99.0 %
  • Chloride (Cl): ≤ 0.002 %
  • Heavy Metals : ≤ 10 ppm
  • Insoluble Matter: ≤ 0.01 %
  • Iron: ≤ 0.002 %
  • Loss on Drying: ≤ 0.2 %
  • pH (5% Solution): 8.7 - 9.3
  • Sulfate: ≤ 0.005 %
  • Aerobic Plate Count: ≤ 1000 CFU/g
  • Yeast and Mold : ≤ 100 cfu/g
  • Total Coliforms: Negative

  

Item#:
ASPHOSODANH
 
PR1MA Biological Buffers
Safety Data Sheet for PR1MA Sodium Phosphate Dibasic, Anhydrous

ION Biosciences Fluo-2 K+ Salt

Green fluorescent, calcium (Ca²+) indicator, membrane impermeable. Higher Ca²+ affinity than Fluo-4.

Fluo-2 is a green fluorescent, calcium (Ca²+) indicator. Fluo-2 has higher Ca²+ affinity (Kd = 290 nM), superior cell loading and comparable spectral properties compared to Fluo-4. Offers superior sensitivity versus other Fluo analogues at lower Ca²+ concentrations.

Fluo-2 K+ salt is a membrane impermeable form of Fluo-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

Item#:
ASFLUO2AMST
 
Fluo-2 K+ Salt Safety Data Sheet
Fluo-2 K+ Salt Product Specifications
ION Biosciences Fura-2 LR K+ Salt
 
Leakage-resistant, ratiometric calcium (Ca²+) indicator, membrane impermeable.
 
UV-excitable, ratiometric green indicator for intracellular calcium (Ca²+) measurements with longer cellular retention compared to Fura-2 AM and similar spectral properties. Ex/Em: 340/505 nm can be used to measure Ca²+-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²+-free Fura-2. Ratiometry is optimal for imaging applications where quantification of intracellular Ca²+ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
 
Fura-2 LR K+ salt is a membrane impermeable form of Fura-2 LR that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Item#:
ASFURA2LRKST
 
LR K+ Salt Safety Data Sheet
LR K+ Salt Product Specifications
ION Biosciences BCECF AM

BCECF AM is the most popular green fluorescent, intracellular pH indicator. BCECF has a pKa of ~7, and exhibits pH-dependent, dual-excitation properties (Ex/Em 430nm/535nm and 490nm/535nm) for ratiometric analysis. Ratiometry is optimal for imaging applications, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology. For HTS applications, BCECF can also be used in non-ratiometric mode using standard fluorescein excitation and emission settings.
Item#:
ASBCECFAMPN
 
BCECF AM Safety Data Sheet
BCECF AM Product Specifications
ION Biosciences Brilliant Sodium Flex Kit
 
ION’s Brilliant Sodium Assay is a total assay solution for multi-well plate-based, high-throughput measurements of changes in intracellular Na+ mediated through a wide-variety of plasma membrane and intracellular sodium channels and transporters. In multi-well, plate-based formats, the Brilliant Sodium Assay can be used to discover and characterize the effects of many tens-of-thousands of compounds and environmental factors on effectors of intracellular Na+. ION’s Brilliant Sodium Assay provides all the reagents necessary for use as a wash or no-wash assay with adherent or non-adherent cells. The optional use of a probenecid solution and an extracellular background masking solution (TRS) offers the ultimate in compatibility for cells types which are difficult to load with fluorescent Na+ indicators (e.g. Chinese Hamster Ovary, CHO cells) and when performing assays in complete, serum-containing cell culture medium is desired.
 
ION’s Brilliant Sodium Assay is compatible with fluorescence microscopes, flow cytometers, and plate readers capable of detecting fluorescein or more optimally, yellow fluorescent protein (YFP).
Item#:
ASBRSOFXK
 
BRILLIANT THALLIUM FLEX_PROTOCOL
ION Biosciences Essentials Red Calcium

Red Calcium Essentials provides the necessary reagents for multi-well plate-based, fluorescence microscopy applications and high-throughput measurements of intracellular calcium (Ca²+) mediated changes through a wide variety of plasma membrane and intracellular calcium channels and transporters. Red Calcium Essentials is also useful for investigating numerous effectors of ion channels and transporters, including G-protein coupled receptors.

Our red calcium dye, ION Calcium Red-1 (ICR-1), is a red fluorescent indicator (Ex/Em: 580/660 nm) responsive to intracellular Ca²+ dynamics. ICR-1’s spectral properties allows for multiplexing with an indicator of another color or GFP-tagged cells.

When following the recommended protocol, Red Calcium Essentials provides enough reagents to make 100 mL of working solution, enough for ten 96-well plates. The actual number of assays will vary according to optimal dye concentrations for your application.
Item#:
ASESSREDCAL
[Lennox L Agar]
 

PR1MA™ LB Agar, Low Salt Formula, Granulated [Lennox L Agar]

Common Name: Lennox L Agar
CAS Number: 91079-40-2, 8013-01-2, 7647-14-5, 9002-18-0
Solubility: Water with Heat
Storage Temp: Room Temperature

For propagation and maintenance of E. Coli.

Dissolve 32 grams in 1 Liter of distilled water and adjust the pH to 7.2. Sterilize by autoclaving for 15 minutes.

Ingredients:

  • Casein Peptone: 10 g/L
  • Sodium Chloride: 5 g/L
  • Yeast Extract: 5 g/L
  • Agar: 12 g/L
Research or further manufacturing use only, not for food or drug use.
  • DCM Appearance: Homogeneous Free Flowing ,Light Beige
  • PPM Appearance: Clear, no to light ppt, light yellow
  • pH (2.0% Solution @ 25 oC): 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Fair to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent
  • Sodium Chloride: 5 g/l
  • Yeast Extract: 5 g/L
  • Agar: 12 g/l
  • Casein Peptone: 10 g/L

Proudly made in the USA! Click here for more made in America products!
  

Item#:
ASLBAGARGRAN
 
PR1MA Growth Media
Safety Data Sheet for PR1MA LB Agar, Low Salt Formula, Granulated [Lennox L Agar]
[Lennox LB Broth]

PR1MA™ LB Broth, Granulated [Lennox LB Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temp: Room Temperature

For propagation and maintenance of E. Coli. Low Salt Formula. Molecular Biology Grade.

Dissolve 20 grams in 1 liter distilled water. Then adjust the pH to 7.2. Heat and frequently agitate to completely dissolve. Material is specially granulated to reduce dust and increase solubility rate. Sterilize by autoclaving for 15 minutes.

Ingredients:

  • Tryptone:10 g/L
  • Sodium Chloride: 5 g/L
  • Yeast Extract: 5 g/L

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Light beige color, average particle size between 1 to 2mm
  • DCM Appearance: Homogeneous, free flowing light beige
  • PPM Appearance: Clear, no to light ppt, light yellow
  • Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
  • pH (2.0% Solution @ 25 oC): 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Fair to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent
  • Casein Peptone: 10 g/L
  • Sodium Chloride: 5 g/L
  • Yeast Extract: 5 g/L

Proudly made in the USA! Click here for more made in America products!
  

Item#:
ASLBROTHGRAN
 
PR1MA Growth Media
Safety Data Sheet for PR1MA LB Broth, Granulated [Lennox LB Broth]

PR1MA™  SOB Broth, Capsules

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 7487-88-9; 7447-40-7
Solubility: Water
Storage Temp: Room Temperature

SOB Broth is recommended for use in cultivation of recombinant strains of E. Coli. It is a nutritionally rich growth medium for use in the preparation and transformation of competent cells.

Add two capsules to one liter of distilled water. Heat and stir gently to completely dissolve medium. Sterilize by autoclaving at 121°C for 15 minutes.

Final Solution contains:

  • Casein Enzymic Hydrolysate: 20 g/L
  • Yeast Extract: 5 g/L
  • Sodium Chloride: 0.5 g/L
  • Magnesium Sulfate: 2.4 g/L
  • Potassium Chloride: 0.186 g/L

Research or further manufacturing use only, not for food or drug use.

  • Appearance: Capsules
  • Appearance of Solution: Prepared medium is light to medium amber and clear
  • pH @25 °C: 6.8 - 7.2
  • Microorganism (E. coli ATCC 23724): Good to Excellent
  • Microorganism (E. coli ATCC 33694): Good to Excellent
  • Microorganism (E. coli ATCC 33849): Good to Excellent
  • Microorganism (E. coli ATCC 39403): Good to Excellent
  • Microorganism (E. coli ATCC 47014): Good to Excellent
  • Microorganism (E. coli ATCC 53868): Good to Excellent

Proudly made in the USA! Click here for more made in America products!
  

Item#:
ASCBROTHSOBCAP
 
PR1MA Growth Media
Safety Data Sheet for PR1MA SOB Broth, Capsules
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