Azure Nitrocellulose Membranes

Pre-cut Nitrocellulose membrane for Western blotting

Low-fluorescence Nitrocellulose membranes are available in both 0.45 μm and 0.22 μm pore sizes to accommodate larger and smaller proteins, respectively. We recommend using the .45 µm pore size for the most analytical blotting: protein, ssDNA, and RNA transfers.

• Low molecular weight proteins
• Typically used for chemiluminescence
• Not ideal for stripping
• Excellent binding properties

Quantity: 10 membranes each   
 
 
 

Azure PVDF Membranes

Pre-cut PVDF membranes for Western blotting applications

Ideal for chemiluminescent and colorimetric Western blotting. Pre-cut to save you time in the lab. These low-fluorescence PVDF membranes reduce background noise and nonspecific protein binding for improved sensitivity. Available in three different pre-cut sizes.

Quantity: 10 membranes per package

Features

Low-fluorescence PVDF membranes reduce background noise for improved sensitivity. PVDF (polyvinylidene difluoride) membranes are commonly used for immunoblotting techniques, like Western blots. During the blotting process, samples are transferred from the protein gel onto a PVDF membrane by electroblotting. From there they can be used for protein sequencing.

• High binding capacity is great for low-expressing proteins
• Typically used for fluorescence
• High protein retention for stripping and reprobing

Azure Blotting Paper for Western Blotting

Pre-cut blotting paper for Western blot transfer

Forget about measuring and cutting blotting paper to the size of your mini-gels. Use these pre-cut papers to assemble your transfer “sandwiches”. Pre-cut blotting papers save time and reduce waste for the easiest Western blotting workflow.

Qty: 60 sheets

Blotting papers are cut to completely cover the gels and membranes.

For 8x10cm gels: blotting papers available in 9x13cm

For 7x9cm gels: blotting papers available in 8x10cm

Features

Pre-cut blotting papers save time and reduce waste for the easiest Western blotting workflow. Forget about measuring and cutting blotting paper to the size of your mini-gels! Use these pre-cut papers to assemble your transfer “sandwiches”.

Blotting papers come pre-cut to completely cover the gels and membranes.   
 
 
 

Azure ChemiWriter ECL Pen

Chemiluminescent pen for blot annotation

The Azure ChemiWriter ECL Western blot annotation pen is a chemiluminescent marker that enables you to write or draw on your transfer membranes, resulting in a chemiluminescent signal on your markings once substrate is added. Contains a reagent that reacts with HRP substrates to produce chemiluminescence and also a blue dye to allow you to see markings as you write on the Western blot.

Use for tracing visible protein ladders or making identifying marks on your Western blots. Pen has two tips on each end: one fine-tipped for thin markings and one thicker for depositing more of the reagent.

Quantity: 1 pen

Features

Enables you to write or draw on your transfer membranes. A chemiluminescent signal will be detectable from your markings once chemiluminescent substrate is added.

• Use for tracing visible protein ladders or making identifying marks on your blots. Pen has two tips on each end: one fine-tipped for thin markings and one thicker for depositing more of the reagent.
• There are two heads to the pen, a fine point tip and a thicker chisel point tip.
• We recommend using the fine point tip to annotate the blot and mark protein ladders for detection.
• The chisel point of the pen can be used to deposit an increased amount of reagent on the blot for more intense signal.
• For best results we recommend annotating your blot after transfer and before starting your Western blotting protocol.
• Once annotated, the blot may be stored dry or wet, refrigerated or at room temperature.
• Storage: pen should be stored tightly closed at room temperature. ChemiWriter ECL reagent and blue ink markings on the blot are stable for at least three months.

Hoefer® Nitrocellulose Membranes

• Ideal for Western Blotting and other immunoblotting techniques
• BSA protein binding capacity of 160 µg/cm2–can bind a wide range of fragment sizes and can still be easily blocked for low background
• High wick rates (lateral flow)–substances move quickly through the membrane

Proudly made in the USA! Click here for more made in America products!
  

Hoefer® PVDF (Polyvinylidene Fluoride) Membranes

• Ideal for use in protein binding applications such as western blots, solid phase assays, and immunoblotting procedures
• Multiple reprobings possible
• Superior strength–can withstand aggressive handling or be used with automated equipment without breaking or tearing
• Low extractables–ensures tests will be clean with consistent results
• Exceptional sensitivity detects low-level components
• Hydrophobic–resists water
• High binding capacity–binds a wide range of fragment sizes

Proudly made in the USA! Click here for more made in America products!
  

Glow-Writer Pen

Fine-Point Phosphorescent Marking Pen for Laboratory Autography

• Fine Point Nib- Provides fine writing and greater information density
• Specially Designed- Ideal glow intensity and afterglow duration helps prevent over-andunder exposure
• No Ink Clogs- Low ink viscosity and porous nib prevents ink from clogging

Protran nitrocellulose membranes are the most frequently specified transfer media in the world for a wide range of applications. Protran membranes are manufactured using 100% pure nitrocellulose to ensure the highest binding capacity possible. Other membranes referred to as "nitrocellulose" may actually contain large amounts of cellulose acetate, which will lower the protein binding capacity. In addition to high binding capacity, Protran nitrocellulose membranes inherently have very low background. The superior surface properties of the membrane guarantees superior signal-to-noise ratios, without the need for stringent washing conditions. Unlike PVDF membranes, Protran nitrocellulose requires no methanol pre-wetting step. This makes it the membrane of choice for proteins that prefer aqueous environments. Prior to transfer, the membrane is simply wet in water and then in the transfer buffer, with no other necessary pre-treatment steps. Protran membranes exhibit the best handling strangth of all pure nitrocellulose membranes.
Features:

• High binding capacity (80-150g/cm2) of proteins and nucleic acids.
• 100% pure nitrocellulose, with no cellulose acetate added, ensures the highest binding capacity possible.
• Compatible with virtually all standard detection methods.
• Low background - NC surface properties guarantee superior signal-to-noise ratios.
• Triton-free matrix with low extractable levels will support the cell growth essential for colony blotting, plaque lifts, and tissue culture applications.
• Available in many sizes and formats to fit different transfer devices and gel chambers.

Pall® FluoroTrans® W PVDF Transfer Membranes
 

• Sensitive Protein Detection with Low Background and Very Low Burnthrough
• Naturally hydrophobic polyvinylidene fluoride is ideal for a wide variety of protein-analysis applications. The family of FluoroTrans® media are white, microporous solid phase supports that bind proteins tenaciously via hydrophobic interactions.
• High sensitivity for small peptides.
• High protein binding capacity. Typically absorbs 50% more protein than nylon or nitrocellulose.

FluoroTrans® W membrane is optimized for Western transfer applications. This membrane allows for sensitive protein detection with low background and very low protein burnthrough. Immobilized proteins can be visually detected with Coomassie blue, Amido Black, Ponceau S, and colloidal gold.

This item has been discontinued.
 

Please contact your trusted MIDSCI Lab Consultant or Email tech@midsci.com for alternate options.

Pall® BioTrace™ NT Nitrocellulose Transfer Membranes

• Pure, Unsupported Nitrocellulose Membrane for Nucleic Acid and Protein Detection
• High binding capacity for proteins and nucleic acids
• Lower protein burnthrough than competitors in electrophoretic transfers

Pall® Biodyne® Nylon Transfer Membranes

• High sensitivity and low background for enhanced detection and resolution.
• Will not crack, shrink, or tear when subjected to multiple cycles of hybridization, stripping, and reprobing
• Membranes are intrinsically hydrophilic for easy wetting
• Offers superior performance with radioactive (Biodyne B membrane) and non-radioactive (Biodyne A membrane) detection systems

Four Chemistries Provide Versatile Adsorption Properties:

• Biodyne A Membrane - (Amphoteric Nylon 6,6) Membrane zeta potential can be modulated by changes in pH. Ideal for single probe or multiple rehybridizations, and applications where background is troublesome.
• Biodyne B Membrane - (Positively-charged Nylon 6,6) Pore surfaces are populated by a high density of quaternary ammonium groups. Our highest sensitivity nylon membrane for nucleic acid applications.
• Biodyne C Membrane - (Negatively-charged Nylon 6,6) Can be derivatized by coupling reactions through the carboxyl groups on the pore surfaces.
• Biodyne Plus Membrane - (Positively-charged Nylon 6,6 with an extremely high isoelectric point) With certain nonradioactive detection systems, it is more sensitive than Biodyne A membrane while exhibiting lower background than Biodyne B membrane.

Pall® FluoroTrans® PVDF Transfer Membranes

• Sensitive Protein Detection with Low Background and Very Low Burnthrough
• Naturally hydrophobic polyvinylidene fluoride is ideal for a wide variety of protein-analysis applications. The family of FluoroTrans media are white, microporous solid phase supports that bind proteins tenaciously via hydrophobic interactions.
• High sensitivity for small peptides.
• High protein binding capacity. Typically absorbs 50% more protein than nylon or nitrocellulose.

FluoroTrans PVDF membrane is optimized for N-terminal protein sequencing. The medium demonstrates good signal-to-noise ratios with standard detection systems, and immobilized proteins can be used directly for sequencing, or visually detected with common staining reagents including Amido Black, Ponceau S, and colloidal gold.