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PR1MA™ Agarose

 

Agarose is a low EEO, multi-purpose, standard melting point agarose that yields high resolution sharp DNA bands with high clarity and low background. Its optimized gel strength enhances ease of gel processing and handling. Manufactured using innovative organic solvent free manufacturing process that is more environment friendly.

 

Application: 

  • Ideal for routine DNA and RNA gel electrophoresis and blotting

 

Features:

  • High purity and Multi-purpose;
  • Low background and high resolution;
  • Optimized gel strength for ease of gel preparation

 

 

Specifications

AppearanceWhite to off-white powder
Water7% max.
SolubilityClear colorless solution at 1G plus
100 mL water
Gel strength(1% Gel)1200 g/cm 2 min.
EEO(Electroendosmosis(-Mr)0.13 max.
Sulfate0.15 max.
Ash0.5 max.
Gelling point (1.5% Gel)36°C±1.5°C
GradeBiotechnology grade
CASNO.9012-36-6

 

 

Item#:
ASMIDSCI500

PR1MA Agarose SS, Super High Strength, Molecular Biology Grade

CAS Number: 9012-36-6
Solubility: Water
Storage Temperature: Room Temperature


For DNA/RNA fragments >1000 bp. Ideal for separating larger molecules. DNAse/RNase free.


Gel Strength, 1%: >1800 g/cm2
Gel Strength, 1.5%: >3200 g/cm2
Gelling Temperature: 35-37°C
Melting Temperature: 87-89°C
Sulfate: <0.12%
EEO: <0.13%


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: Fine white homogeneous powder
  • DNase/ RNase: Not Detected
  • Gelling Temperature:  10 oC
  • Melting Point: 80 - 100 oC

Item#:
ASPRIMAAGASHS

PR1MA Agarose 500mg Tablets

CAS Number: 9012-36-6
Solubility: Water
Storage Temperature: Room Temperature


Save time and labor preparing agarose gels for electrophoresis of nucleic acids. Just place the required number of tablets into a standard buffer, then mix and heat until completely dissolved.


Tablet Weight Range: 485-515 mg
Gel Strength, 1.5%: >1200 g/cm2
Gelling Range: 36-39°C
Melting Temp: 87-89°C
EEO: <0.10
Sulfate: <0.15
DNase, RNase: None Detected


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White tablets
  • Gelling Range (1.5% Water): 36 - 39 oC
  • pH (3% water, 25°C): 5.7 - 7.7
  • Tablet Weight: 485 - 515 mg

PR1MA Agarose 3:1, Molecular Biology Grade

CAS Number: 9012-36-6
Solubility: Water
Storage Temperature: Room Temperature


Molecular Biology Grade. For DNA/RNA fragments 150-1500bp. Exceptional gel strength. DNase/RNase free.


Gel Strength, 4%: 
≥ 1400 g/cm2
Gelling Temperature (4%): 32-28°C
Melting Temperature (4%): 90°C
Sulfate: <0.15%
EEO: <0.13


Research or further manufacturing use only, not for food or drug use.

 

  • Appearance: White to Off White Powder
  • EEO:  0.13 -Mr
  • Gelling Temperature: 32.5 - 38.0 oC
  • Gel Strength :  1400 g/cm2
  • Melting Temperature:  90.0 oC
  • Moisture Content:  10.0 %
  • Sulfate:  0.15 %

PR1MA  Agarose, Low EEO

CAS Number: 9012-36-6
Solubility: Water
Storage Temperature: Room Temperature


Molecular Biology Grade. High purity for preparative and analytical electrophoresis of nucleic acids >1000 bp.


Gelling Temperature: 34.5 -37.5°C
Sulfate: 0.15
EEO: 0.09- 0.13
DNA Binding: None Detected
DNA and RNase: None Detected


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: Powder
  • DNase/ RNase: Not Detected
  • EEO: 0.09 - 0.13
  • Gelling Point: 34.5 - 37.5 °C
  • Gel Strength (1.0%):  1200 g/cm²
  • Moisture Content:  10.0 %
  • Sulfate:  0.15 %


EZ Pack Agarose Tablets

  • Eliminates weighing
  • Fast dissolving, just two minutes
  • Environmentally friendly, free from organic solvents
  • Consistent, reproducible gels
  • Enhanced resolution and clarity 

Researchers trust Benchmark’s Agarose LE to meet their electrophoretic needs for various applications. Now, the same agarose is available in a convenient, no-mess tablet.


Agarose LE is refined in an advanced process that excludes the use of organic solvents. The result is a cleaner end product with significantly reduced environmental impact. The agarose can be used for analyses of nucleic acids from 150 bp to 25,000 bp, protein electrophoresis, and various blotting protocols.


The low EEO of the agarose promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO reduces band distortion (caused by counterflow) that can result from the presence of excessive sulfate-rich negative ions. The quick-dissolving EZ Pack™ tablet contains 0.5 g (500 mg) of Agarose LE, eliminating the hands-on time and inaccuracies normally associated with weighing. Use of the tablet is not only convenient and cleaner, but it also provides better consistency and reproducibility gel to gel.

 

Gel preparation is simple – add the desired number of tablets to the electrophoresis buffer, allow it to sit for two minutes, and then heat and pour as usual. The resulting gels are highly transparent, have exceptional thermal stability (ensuring safe and easy handling), and exhibit exceptionally low absorption of chemical staining agents.

EZ Pack™ Agarose Tablets are supplied in convenient blister packs for safe, clean dispensing.

 

Specifications
 

Gel Temperature36°C ± 1.5°C (1.5%)
Melt Temperature88°C ± 1.5°C (1.5%)
EEO (-mr)< 0.13
Moisture Content< 10%
Sulfate< 0.2%
RNase/DNaseNone Detected
Protease/EndonucleaseNone Detected
Storage ConditionsRoom Temperature
Gel Strength> 1200 g / cm2 (1%)
> 2500 g / cm2 (1.5%)
 
Buffer volume required to achieve desired gel strength
GEL %0.8%1.0%1.2%1.3%1.5%1.8%2.0%
1 Tab63 mL50 mL42 mL38 mL33 mL28 mL25 mL
2 Tabs125 mL100 mL83 mL77 mL67 mL56 mL50 mL
3 Tabs188 mL150 mL125 mL115 mL100 mL83 mL75 mL

Item#:
ASAGROTBLTS

Kappa is now PR1MA - Same great product, new name
Looking for something else? Email tech@midsci.com. If we can get it, we will add it to the website.

 

PR1MA Agarose, Low Melt Temperature

CAS Number: 9012-36-6
Solubility: Water
Storage Temperature: Room Temperature


Ideal gelling properties for use in cloning assays or for recovery of heat labile samples following electrophoresis.


Gel Strength, 1%: >200 g/cm2
Gelling Temperature: 26-30°C
Melting Temperature: <65°C
Sulfate: <0.10%
EEO: <0.10
DNase and RNase: None Detected


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: White to off white powder
  • DNase/ RNase: Not Detected
  • EEO:  0.10 -Mr
  • Gelling Temperature: 26 - 30 oC
  • Gel Strength :  200 g/cm2
  • Melting Temperature:  65 oC
  • Moisture Content:  10 %
  • Sulfate Content:  0.10 %

Bullseye Agarose General Purpose

 

Bullseye Molecular Biology Grade Agarose is certified for use in standard electrophoresis procedures, including nucleic acid analysis, preparative electrophoresis, and blotting. This highly refined and purified agarose is produced under strict testing parameters to ensure lot-to-lot consistency. It features low electrophoretic mobility characteristics, making it ideal for a variety of applications.

 

Extracted from the cell walls of certain Rhodophyceae (agarophyte seaweeds), Bullseye Standard Agarose is a neutral polysaccharide composed of galactan, linked by agarobioses in a 1-3, 1-4 arrangement. This unique chemical structure enables agarose to form robust gels even at low concentrations. The gels exhibit a macroreticular structure with an open mesh, easily adjustable by varying the agarose concentration.

 

Bullseye Agarose GP2 offers:

  • General-purpose electrophoresis and blotting capabilities
  • Excellent separation, resolution, and clarity
  • Low absorption and background from staining reagents
  • High gel strength for safe and easy handling
  • Superior refinement and purification
  • Enhanced thermal stability
  • Comprehensive packaging including a specification sheet, preparation instructions, and MSDS
  • Choose Bullseye Standard Agarose for reliable performance in your molecular biology applications.

 

 

Physical Specifications:

CAS#: 9012-36-6
Moisture: ≤ 7%
Ash: ≤ 0.4%
EEO: 0.05-0.13
Sulfate: ≤ 0.10%
Clarity @ 1.5% (NTU): ≤ 3
Gel Strength @ 1.0% (g/cm2): ≥ 1200
Gel Strength @ 1.5% (g/cm2): ≥ 2500
Gelling Temperature @ 1.5%: 36°C ± 1.5°C
Melting Temperature @ 1.5%: 88°C ± 1.5°C

 

 

Molecular Biology Specifications:

DNase assay: None Detected
RNase assay: None Detected
Protease assay: None Detected
Gel analysis assay: Pass
DNA Resolution: ≥ 1000bp (Finely Resolved)
DNA Extraction assay: Pass
Gel Background: Very Low

 

 

Base Pair Data
1X TAE BufferGel Concentration %1X TBE Buffer
50-1500bp250-1,000bp
100-2500bp1.75100-2,000bp
200-5000bp1.5150-3,000bp
250-10,000bp1.25200-4,000bp
300-16,000bp1300-8,000bp
500-20,000bp0.75500-12,000bp
Item#:
ASPCRAGAROSE1
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