NEST High-Efficiency Erlenmeyer Flasks

High-efficiency, large-volume culture flasks allow cells to show strong viability with large expression amounts of proteins in the culture of mammalian cells and insect cells. During the culturing process, the use rate of the shaker is significantly increased, and the survival rate and viability of cells are both dramatically elevated.

NEST culture flasks also provide high repeatability, which allows high inter-batch consistency of cell growth and yield.

3 L Wide-mouth Efficient Erlenmeyer Flask

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Erlenmeyer Flasks

Erlenmeyer Shaker Flasks are made of polycarbonate, PETG or styrene/butadiene (Jumbo Flasks only). The polypropylene screw caps are vented with 0.2µm membranes to increase air transmission and enhance cell growth. The mouth size conforms with the international SBS standard, for quick, secure connection to bioreactor equipment.

Polycarbonate flasks and Jumbo Fernbach flasks have baffled (ribbed) bottoms to provide increased oxygenation and mixing efficiency even at lower shaking speeds. All flasks, regardless of size, are individually wrapped and gamma sterilized to SAL 10-6, and they are free of RNase, DNase, pyrogen, endotoxins and human DNA.

Polycarbonate Flasks are autoclavable at 121°C and 15PSI for 30 minutes.

• Vented screw caps
• Flat or baffled bottoms
• Gamma sterile (SAL 10-6)
• Pyrogen-free and RNase/DNase free
• Individually wrapped 

NEST Transfer Cap for High Efficiency Erlenmeyer Flasks

Two-Way Liquid Transfer Cap
The two-way transfer cap is used along with a 2L, 3L or 5L shake flask to connect a liquid inlet tubing with the required device. The liquid transfer is achieved by connection of a peristaltic pump between the shake flask and the device. Upon completion of the transfer, the transfer cap can be replaced with a vent cap for culture.

Multifunctional Liquid Transfer Cap
Unlike the two-way liquid transfer cap, the multifunctional transfer cap can be directly placed in an incubator for culture after the liquid transfer is completed. It can reach a large air flux. The sampling part is composed of a sampling nozzle and a one-way valve, which can prevent the liquid from flowing backwards during the sampling process and ensure the aseptic sampling. The liquid inlet tubing is provided with a PTFE needle filter, which solves the issue of liquid remaining in the tubing during the feeding process.

Inverted Liquid Transfer Cap
The inverted transfer cap is used along with a 2L, 3L or 5L shake flask to connect a liquid inlet tubing with the required device. When liquid transfer is required, the liquid is transferred under gravity with the inverted shake flask.

Application

It is applicable to liquid transfer and culture during mass proliferation of bacteria and suspension cells.

Product Features

• Closed transfer reduces the risks of contamination during liquid transfer
• The cap is molded and connected in an integrated manner, reducing the risks of leakage and media residues
• A variety of tubing diameters are available and aseptic welding of liquid inlet tubing under normal conditions is supported
• High-quality materials and smooth inner wall of the tubing provide an excellent transfer performance
• Sterility level (SAL) is maintained at 10-6
• Endotoxin-free, and no ingredients of animal origin

Small Erlenmeyer Flask