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Molecular Biology Reagents/Chemicals

MIDSCI™ gives you ultra-pure, cost-competitive molecular biology reagents and lab chemicals for life science research; often with significant cost savings over Sigma Aldrich and other lab reagent providers.

PR1MA™ Chemicals can help with a wide variety of research-based requirements.
 
Use our glycerol for polyacrylamide gel electrophoresis. Eliminate Agrobacterium after inoculation with the carbenicillin we carry. Or detect β-Galactosidase activity with X-GAL which, when combined with the IPTG we stock, can be used to detect lac gene expression in cloning applications.
 
We also carry Luria Broth (Lennox), for the propagation and maintenance of E. coli; DTT, in order to maintain –SH groups in a reduced state; and high gel strength agar for tissue culture and microbiological work.

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PR1MA™ Agarose

 

Agarose is a low EEO, multi-purpose, standard melting point agarose that yields high resolution sharp DNA bands with high clarity and low background. Its optimized gel strength enhances ease of gel processing and handling. Manufactured using innovative organic solvent free manufacturing process that is more environment friendly.

 

Application: 

  • Ideal for routine DNA and RNA gel electrophoresis and blotting

 

Features:

  • High purity and Multi-purpose;
  • Low background and high resolution;
  • Optimized gel strength for ease of gel preparation

 

 

Specifications

AppearanceWhite to off-white powder
Water7% max.
SolubilityClear colorless solution at 1G plus
100 mL water
Gel strength(1% Gel)1200 g/cm 2 min.
EEO(Electroendosmosis(-Mr)0.13 max.
Sulfate0.15 max.
Ash0.5 max.
Gelling point (1.5% Gel)36°C±1.5°C
GradeBiotechnology grade
CASNO.9012-36-6

 

 

Item#:
ASMIDSCI500

PR1MA™ Distilled Water, Laboratory Reagent Grade

CAS Number: 7732-18-5
Molecular Weight: 18.02
Chemical Formula: H2O


Laboratory reagent grade. Purified by steam distillation for use as a research laboratory reagent. Not intended or sold for human consumption. 10 Liter size supplied in a Cubitainer with pouring spout.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Clear Liquid
  • Alkalinity (Total as CaCO3): ≤ 20 ppm
  • Aluminum (Al): ≤ 0.2 ppm
  • Antimony (Sb): ≤ 0.006 ppm
  • Arsenic (As): ≤ 0.01 ppm
  • Barium (Ba): ≤ 2 ppm
  • Beryllium (Be): ≤ 0.004 ppm
  • Boron (B): ≤ 1 ppm
  • Bromate: ≤ 0.010 ppm
  • Bromide: ≤ 0.005 ppm
  • Cadmium (Cd): ≤ 0.005 ppm
  • Calcium (Ca): ≤ 2 ppm
  • Chloride (ASTM D5442): ≤ 50 ppb
  • Chromium (Cr): ≤ 0.10 ppm
  • Cobalt (Co): ≤ 0.15 ppm
  • Conductivity (ASTM D1125): ≤ 5.0 µS/cm
  • Copper (Cu): ≤ 1 ppm
  • E.Coli: ≤ 1 MPN/100ml
  • Fluoride : ≤ 4 ppm
  • Foaming Agents: ≤ 0.5 mg/l
  • Hardness (as CaCO3): ≤ 10 mg/l
  • Heterotrophic Bacteria (F1094): ≤ 100 cfu/10ml
  • Iron (Fe): ≤ 0.3 ppm
  • lead (Pb): ≤ 0.015 ppm
  • Magnesium (Mg): ≤ 0.10 ppm
  • Manganese (Mn): ≤ 0.05 ppm
  • Mercury (Hg): ≤ 0.002 ppm
  • Molybdenum (Mo): ≤ 0.15 ppm
  • Nickel (Ni) : ≤ 0.005 ppm
  • Nitrate as N: ≤ 10 ppm
  • pH (ASTM D1293): 5.0 - 7.0
  • Phosphorous (P): ≤ 1 ppm
  • Potassium (K) : ≤ 1 ppm
  • Resistivity (ASTM D1125): ≥ 0.2 megaohm
  • Scandium (SC): ≤ 0.15 ppm
  • Selenium (Se): ≤ 0.05 ppm
  • Silica (ASTM D4517) : No Limit ppb
  • Silver (Ag): ≤ 0.10 ppm
  • Sodium (ASTM 6071): ≤ 50 ppb
  • Standard Plate Count: ≤ 500 cfu/ml
  • Strontium (Sr): ≤ 0.15 ppm
  • sulfate: ≤ 250 ppm
  • Thallium (Tl): ≤ 0.002 ppm
  • Tin (sn): ≤ 0.15 ppm
  • Titanium (Ti): ≤ 0.15 ppm
  • TOC (ASTM 5997): No Limit ppm
  • Total Coliform: ≤ 1 MPN/100ml
  • Total Radium: 0.5835 - 1.491 pCi/l
  • Uranium (U): ≤ 0.030 ppm
  • Vanadium (V): ≤ 0.15 ppm
  • Zinc (Zn) : ≤ 5 ppm
Item#:
ASWATERDISTILL
 
Safety Data Sheet for PR1MA Distilled Water, Laboratory Reagent Grade

PR1MA™ Water, HPLC Grade

CAS Number: 7732-18-5
Molecular Weight: 18.0
Chemical Formula: H2O
Storage Temperature: Room Temperature


Certified RNase and DNase free for use in molecular biology applications. Ideal for making reagents, rinsing glass and plastic ware, enzymatic reactions and more. Toxic agents such as DEPC are not used in purification procedures.

  • Endotoxin Free
  • RNase Free
  • DNase Free


*Tested to < 0.001 EU/ML


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Clear and Colorless Solution
  • TOC (ppb): ≤ 3 ppb
  • UV Absorbance (1 cm cell Vs Water) @ 190nm: ≤ 0.01
  • UV Absorbance (1 cm cell Vs Water) @ 200 nm: ≤ 0.010
  • UV Absorbance (1 cm cell Vs Water) @ 210 nm: ≤ 0.010
  • UV Absorbance (1 cm cell Vs Water) @ 250 nm: ≤ 0.005
  • UV Absorbance (1 cm cell Vs Water) @ 254 nm: ≤ 0.005
  • UV Absorbance (1 cm cell Vs Water) @ 280 nm: ≤ 0.005
  • Filtered through Ultrafiltration (0.01 Micron): Conforms
  • Country of Origin: USA
Item#:
ASEATERHPLC
 
Safety Data Sheet for PR1MA Water, HPLC Grade

PR1MA™  Water, Molecular Biology Grade, DNase and RNase Free

CAS Number: 7732-18-5
Molecular Weight: 18.0
Chemical Formula: H2O
Storage Temperature: Room Temperature


Certified RNase and DNase free for use in molecular biology applications. Ideal for making reagents, rinsing glass and plastic ware, enzymatic reactions and more. Toxic agents such as DEPC are not used in purification procedures.
 

  • Endotoxin Free
  • RNase Free
  • DNase Free


*Tested to < 0.001 EU/ML


Meets or exceeds CAP/CLSI specifications for Clinical Laboratory Reagent Water (CLRW) and USP/NF Purified Water.


Research or further manufacturing use only, not for food or drug use.
 

  • Appearance: Clear and Colorless Solution
  • Bioburden : No Growth
  • DNase: No Visible Degradation
  • Protease: No Visible Degradation
  • RNase: No Visible Degradation
  • Country of Origin: USA
Item#:
ASWATERMBG
 
Safety Data Sheet for PR1MA Water, Molecular Biology Grade

PR1MA™ Boric Acid

CAS Number: 10043-35-3
Molecular Weight: 61.83
Chemical Formula: H3BO3
Solubility: Water
Storage Temperature: Room Temperature


For use in preparing electrophoresis buffers.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White Powder
  • Calcium (Ca): ≤ 0.005 %
  • Chloride: ≤ 0.001 %
  • Heavy Metals (as Pb): ≤ 0.001 %
  • Insolubles (Methanol): ≤ 0.005 %
  • Iron: ≤ 0.001 %
  • Nonovolatiles (Methanol): ≤ 0.05 %
  • Phosphate: ≤ 0.001 %
  • Purity: ≥ 99.5 %
  • Sulfate: ≤ 0.01 %

Item#:
ASBORICACID
 
Safety Data Sheet for PR1MA Boric Acid

PR1MA™ Guanidine Thiocyanate

CAS Number: 593-84-0
Molecular Weight: 118.2
Chemical Formula: CH5N3 • HSCN
Solubility: Water
Storage Temperature: Room Temperature


Protein denaturant used for purifying proteins and nucleic acids.


Toxicity: LD50


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White to slightly off white crystalline powder
  • Assay (Argentometric Titration): ≥ 99.0 %
  • Assay (Ion Chromatography): ≥ 99.5 %
  • Chloride Content: ≤ 1.0 %
  • Cobalt (Co) : ≤ 5 ppm
  • FTIR: Conforms to refference
  • Iron: ≤ 5 ppm
  • pH: 5.0 - 7.0
  • DNase/ RNase: None Detected
  • Melting Point: 118 - 121 oC
  • Solubility: Pass
  • Sulfate Ash: ≤ 0.5 %
  • Moisture : ≤ 0.5 %
  • Grade: Ultra Pure
  • Country of Origin : Report

Item#:
ASGUANIDINETHIO
 
Safety Data Sheet for PR1MA Guanidine Thiocyanate

PR1MA™ Guanidine Hydrochloride

CAS Number: 50-01-1
Molecular Weight: 95.5
Chemical Formula: CH5N3-HCl
Solubility: Water
Storage Temperature: Room Temperature


Protein denaturant used for purifying proteins and nucleic acids.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White Crystalline Powder
  • Assay (Titration, dried basis): ≥ 99.5 %
  • Copper (Cu): ≤ 1 ppm
  • DNase, RNase, protease: Not Detected
  • Free Acid: ≤ 0.01 %
  • Heavy Metals (as Pb): ≤ 0.5 ppm
  • Identification (IR): Conforms to Known Structure
  • Iron (Fe): ≤ 1 ppm
  • Loss on Drying: ≤ 1 %
  • Melting Point: 185 - 188 oC
  • pH (10% solution in H2O @ 25oC): 5.0 - 7.0
  • Residue on Ignition: ≤ 0.05 %
  • Solubility (6% Solution in H2O): Clear
  • UVA (230nm, 6M H2O solution): ≤ 0.14
  • UVA (240nm, 6M H2O solution): ≤ 0.05
  • UVA (260nm, 6M H2O solution): ≤ 0.03
  • Zinc (Zn): ≤ 1 ppm

Item#:
ASGUANIDINEHYDRO
 
Safety Data Sheet for PR1MA Guanidine Hydrochloride
[Tris(hydroxymethyl) aminomethane HCl]

PR1MA™ TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl]

Common Name: Tris(hydroxymethyl) aminomethane hydrochloride
CAS Number: 1185-53-1
Molecular Weight: 157.6
Chemical Formula: C4H11NO3 • HCl
Solubility: Water
Storage Temperature: Room Temperature


Highly purified for use in molecular biology applications.


Research or further manufacturing use only, not for food or drug use.


  • Appearance: White crystalline
  • Assay: 99.0 - 101.0 %
  • pH (1% aqueous solution): 4.2 - 5.0
  • Solubility (1M Solution in Water ) : Clear, colorless solution
  • UV Absorbance @260nm, 1.0 M in H2O: ≤ 0.06
  • UV Absorbance @280nm, 1.0 M in H2O: ≤ 0.05
  • Identification: Conforms
  • Heavy Metals : ≤ 5 ppm
  • Grade: USP

Item#:
ASTRIS
 
Safety Data Sheet for PR1MA TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl]

PR1MA™  Tricine, Ultra Pure [N-[Tris (Hydroxymethyl) Methyl] Glycine]

Common Name: N-[Tris (Hydroxymethyl) Methyl] Glycine
CAS Number: 5704-04-1
Molecular Weight: 179.17
Chemical Formula: C6H13NO5
Solubility: Water
Storage Temperature: Room Temperature
 

  • pKa @ 25°C: 8.1-8.2
  • Melting Range: 182-188°C

Research or further manufacturing use only, not for food or drug use.


  • Appearance: White Crystalline Powder
  • Assay (Titration, dried basis): ≥ 99.0 %
  • Heavy Metals : ≤ 5 ppm
  • pH (1% Solution): 4.6 - 5.3
  • Solubility (1M Solution in H2O): Clear
  • UV Absorbance 260nm (1 mol/L, 1cm ): ≤ 0.04
  • UV Absorbance 280nm (1 mol/L, 1cm ): ≤ 0.02
  • Water Content : ≤ 0.1 %
Item#:
ASTRICINEUP
 
PR1MA Biological Buffers
Safety Data Sheet for PR1MA Tricine, Ultra Pure

PR1MA™ Ampicillin, Sodium Salt


CAS Number: 69-52-3
Molecular Weight: 371.4
Chemical Formula: C16H18N3O4SNa
Solubility: Water
Storage Temperature: 2-8°C
 
 

Inhibitor of bacterial cell wall synthesis resulting in lysis or deformation of the bacterium. Inhibits crosslinking of peptidoglycan by binding and inactivating transpeptidases. Highly active against gram negative bacteria. B-Lactamase sensitive. Plant cell culture tested. Widely used as a selective agent for transformation of plasmid encoding for b-Lactamase production such as pBR322 or pUC (Ampr).
 
 

  • Appearance: White Crystalline Powder
  • Assay (Anhydrous Substance) : 91.0 - 102 %
  • Bacterial Endotoxins : ≤ 0.15 IU/mg
  • Dimethylaniline: ≤ 0.002 %
  • Identification: Conform
  • Methylene Chloride : ≤ 0.2 %
  • pH (1% Solution): 8.0 - 10.0
  • Solubility (50mg/ml in Water): Soluble
  • Specific Optical Rotation: +258 - +287 °
  • Sterility: Conform
  • Water: ≤ 2.0 %
Item#:
ASAMPICILLIN
 
PR1MA Antibiotics
Safety Data Sheet for PR1MA Ampicillin, Sodium Salt

PR1MA™ Potassium Chloride

CAS Number: 7447-40-7
Molecular Weight: 74.6
Chemical Formula: KCl
Solubility: Water
Storage Temperature: Room Temperature


Research or further manufacturing use only, not for food or drug use.


  • Appearance: Colorless Crystals or White Granular
  • Acidity/Alkalinity: Pass Test
  • Aluminium: ≤ 1 ppm
  • Assay: 99.0 - 100.5 %
  • Barium: Pass Test
  • Bromides: ≤ 0.1 %
  • Heavy Metals (as Pb): ≤ 10 ppm
  • Identity Reaction: Pass Test
  • Iodide: ≤ 50 ppm
  • Loss on Drying: ≤ 1.0 %
  • Sodium: ≤ 1000 ppm
  • Solubility: Freely soluble in water, practically insoluble in anhydrous ethanol
  • Sulfate : ≤ 300 ppm
  • Grade: Meets current USP Specifications
Item#:
ASPOTASSCHLOR
 
Safety Data Sheet for PR1MA Potassium Chloride

PR1MA™ TBE 10x Solution


Common Name: TRIS-borate-EDTA 10X Solution

CAS Number: 77-86-1; 10043-35-3; 6381-92-6; 7732-18-5

Solubility: Water

Storage Temperature: Room Temperature


TBE is a common buffer used in gel electrophoresis.

Supplied as a 1X concentrated solution containing:

89mM Tris Base
89mM Boric Acid
2mM EDTA disodium salt
pH (1X solution @ 25°:C): 8.3+0.1
Shipping Weight: 2.6 lbs

Shipping Dimensions: 3.50 x 9.00 x 3.50

  • Appearance: Clear, Colorless Solution
  • pH (1X Solution): 8.15 - 8.45

Research or further manufacturing use only, not for food or drug use.
Item#:
ASTBE10XSOLUT
 
Safety Data Sheet KCT32020-1000_T32020
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