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TPP Plastics
MIDSCI carries an extensive selection of superior quality TPP plastics built to boost cell health and provide reliable experimental results.
Our line of TPP products, including cell culture plasticware, are made from the highest purity TPP plastics and are innovatively modeled to improve the generation of consistent and reproducible data.
Ergonomically designed to offer better comfort and sample handling, our TPP plasticwares secure optimal cell retrieval for more efficient downstream applications.
Our TPP plastics, including cell and tissue cultureware, offer a flatter, more consistent growth surface for a more consistent monolayer as well.
Furthermore, insulated vents ensure even temperature distribution when stacked.
Finally, ridged gripping surface on dishes and plates facilitate handling, high visibility, painted alphanumerics, graduations and writing surfaces.
TPP's Packed Cell Vol (PCV) tube enables a quick and reproducible method of cell counting based on cell Vol. A quick centrifuge of the cell suspension pipetted into a PCV tube reveals a packed cell pellet in the PCV capillary, which can be easily read with the aid of an Easy read ruler. This non-subjective method is superior to old-fashioned and labor intensive hemacytometer based counting.
Functional Features:
- Cell suspension sample can be directly quantitated without dilution
- Multiple samples can be assayed simultaneously
- Load and spin as many PCV tubes as your centrifuge rotor holds
- Quick and reproducible
- Cells can be spun down in 1 minute at 2500xg
Ergonomic Features:
- Reproducible measurements
- Graduated capillary model offers immediate quantitation
- For finer quantitation: The Easy Read Ruler is used with non-graduated PCV tubes
- Eliminates expensive automated counters / fragile slides
- Avoids subjective microscope counting
Quality Assurance:
- Gamma-sterilized, DNAse/RNAse-Free, and pyrogen-Free
- Individually wrapped in peel-off plastic wrapper
Protocol Example:
- Uniformly mix cell suspension collected from TPP flask/dish/bioreactor
- Pipet 1000µL of cell suspension into a PCV tube
- Spin multiple samples for 1 minute at 2500xg in a standard microcentrifuge
- Record pellet Vol and compare to pre-generated standard curve of cell numbers to packed cell Vol.
- Standard curve needs to be set up only once per cell line
- Pipet pre-determined cell suspension Vols (dilutions) into a hemacytometer
- Calculate and record the number of live cells for each concentration
- Pipet the identical Vols into the PCV tube, centrifuge and record the numeric in the capillary that corresponds to pellet Vol
- Plot the hemacytometer readings vs. the PCV readings for each Vol
- This Standard Curve doesn't need to be redone for the same cell line and moving forward the cell suspension harvested from TPP flasks/dishes/plates only need to be centrifuged in PCV tubes and assayed for cell pellet measurements
High density of cells
- With a very high density of the cells, the samples must not be diluted. You might simply reduce the sample Vol. Samples from the same series should be treated accordingly.
- For bacteria measurement the parameters have to be adjusted, for example higher g-force, smaller Vol, etc.
- Micro centrifuges with swing-out rotors are preferable.
Order# |
Description |
Quantity |
TP87005 |
PCV Cell Counting Tubes (with capillary graduations only) |
50/pk |
TP87007 |
PCV Cell Counting Tubes (no graduations) |
50/pk |
TP87008 |
TPP Cap for PCV Tubes |
50/pk |
TP87010 |
Easy Read Ruler |
1 |
Please call us for sample!
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