PR1MA™ Protein Gel-Loading Dye for SDS-PAGE

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Azure ChemiWriter ECL Pen

Chemiluminescent pen for blot annotation

The Azure ChemiWriter ECL Western blot annotation pen is a chemiluminescent marker that enables you to write or draw on your transfer membranes, resulting in a chemiluminescent signal on your markings once substrate is added. Contains a reagent that reacts with HRP substrates to produce chemiluminescence and also a blue dye to allow you to see markings as you write on the Western blot.

Use for tracing visible protein ladders or making identifying marks on your Western blots. Pen has two tips on each end: one fine-tipped for thin markings and one thicker for depositing more of the reagent.

Quantity: 1 pen

Features

Enables you to write or draw on your transfer membranes. A chemiluminescent signal will be detectable from your markings once chemiluminescent substrate is added.

• Use for tracing visible protein ladders or making identifying marks on your blots. Pen has two tips on each end: one fine-tipped for thin markings and one thicker for depositing more of the reagent.
• There are two heads to the pen, a fine point tip and a thicker chisel point tip.
• We recommend using the fine point tip to annotate the blot and mark protein ladders for detection.
• The chisel point of the pen can be used to deposit an increased amount of reagent on the blot for more intense signal.
• For best results we recommend annotating your blot after transfer and before starting your Western blotting protocol.
• Once annotated, the blot may be stored dry or wet, refrigerated or at room temperature.
• Storage: pen should be stored tightly closed at room temperature. ChemiWriter ECL reagent and blue ink markings on the blot are stable for at least three months.

Azure Western Blot Quenching Sheets

Background quenching sheets for use with fluorescent or chemiluminescent samples

Use these quenching sheets under your blot when imaging fluorescent or chemiluminescent Western blots. Sheets reduce background autofluorescence or background signal from contamination in shared imagers, improving the quality of your image.

• For use with fluorescent or chemiluminescent samples.
• Size: 9 x 12.5 cm
• Quantity: 10 Sheets

Features

Use these quenching sheets under your blot when imaging fluorescent or chemiluminescent Western blots.

The sheets reduce background autofluorescence or background signal from contamination in shared imagers, improving the overall quality of your image.

 
Western Blot Boxes

• The perfect box for every blot
• Reduce antibody usage
• The hinged Lid minimizes evaporation
• For halves, wholes, strips, or minis

No matter what size your Western blot is, there's a box that fits it perfectly. With boxes that are correctly matched to common blot sizes, you can fit more blots on shakers/rockers while keeping reagent and antibody usage to an absolute minimum. Additionally, all corners are formed at 90° angles and the bottom surface is uniformly flat, further reducing consumption of costly reagents and antibodies (for example, popular mini gels require only about 4 mL).

At a price that makes them truly disposable, all sizes are engineered from crystal clear polystyrene. The exception is our opaque black boxes (except WBB7BK and WBB15BK), which protect light-sensitive samples. All sizes are also equipped with an easy-to-open hinged lid that reduces evaporation.

Specifications
 

This brand is being discontinued and will only be available while supplies last.

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Try our PR1MA™ SmartCheck DNA Ladders

Bullseye Pre-stained Protein Ladder, 10-180 kDa

• Ready to use - no boiling necessary
• Stable at ambient temperature
• 2 reference bands - red at ~75kDa and green at ~25kDa
• Use 5-15µl, depending on the size of the wells
• No freeze-thaw involved
• Broad range ladder

Application: The Bullseye Pre-stained Protein Ladder is suitable for visualizing proteins during electrophoresis without staining and for monitoring transfer onto membrane.

The Bullseye Pre-stained Protein Ladder contains 10 prestained proteins, covering a wide range of molecular weights from 10 to 180kDa. It is designed for monitoring protein separated during polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes and for sizing of proteins. The ladder is supplied in protein gel loading buffer and is ready-to-use. No need to heat, dilute, or add reducing agent before loading. One prominent feature of this protein ladder, is that it remains in the liquid state at the storage temperature; therefore, there is not a freeze-thaw cycle involved, making the ladder very stable.

Contents:
0.1~0.4 mg/ml of each protein in 20 mM Trisphosphate (pH 7.5 at 25°C), 2% SDS, 1 mM reducing reagent, 3.6 M Urea, and stabilizer.

Usage:
For each application, 5-15µl is needed, depending on the size of the wells.

Quality Control:
Tested in polyacrylamide gel electrophoresis and Western Blotting.

Storage: -20 °C.

Note for image:

Western Lightning Plus (NEL103):
·  Twice the sensitivity of standard products 

·  Direct protocol transfer 

·  ~ 1 picogram sensitivity

·  > 2 hr signal duration for re-exposing film

·  Working solution stability 8 hr at room temperature 

·  PVDF or Nitrocellulose membranes 

·  Non-toxic (no msds required)

Western Lightning Pro (NEL120)
·  Mid-range sensitivity, with broad dynamic range

·  Detection of less than 1 picogram amount of protein

·  Long, stable signal > 12 hr for re-exposing film 

·  Stable at room temperature >1 year 

·  Working solution stable for > 24 hr at room temperature

·  Non-toxic (no msds required) 

Western Blot 2.0 with PURITY™

The PURITY™ detection system revolutionizes Western Blotting in three steps.
 

Blocking buffer and secondary antibody are already components of the PURITY™ Reagent. And the washing buffer is also included. The only thing to add is your specific primary antibody.

Benefits:

• 1 simple protocol which makes almost all optimization unnecessary.
• Increased sensitivity and reproducibility of blots.
• Dramatically reduces the number of steps and time required for Western blots.
• Drastically decreases background phenomena.
• Highly reliable.

• Used for agarose electrophoresis of DNA, RNA or nucleic acids
• Contains 3 tracking dyes and 15% Ficoll in a special Tris dye

• Light blue - around 4000bp in 1% agarose
• Indigo - around 600bp in 1% agarose
• Magenta - around 150bp in 1% agarose

• DNase/RNase/Protease free

IB01015
5X RNA Gel Loading Kit

• Reagents for denaturing and loading RNA samples onto a formaldehyde gel, using MOPS as a buffer
• RNA sample is dissolved in 10µl of DEPC water and mixed with 35µl of denaturing solution. Heat the sample to 65°C for 5 min. Once the solution has cooled, add 5µl of loading dye. The sample is now ready to load into the gel.
• DNase/RNase/Protease free

IB01190
2X Protein Loading Dye

• Tracks the migration progression of your sample during polyacrylamide electrophoresis
• Loading dye migrates independently of the samples, making it easier to estimate the migration of proteins
• DNase/RNase/Protease free

IB72120
Xylene Cyanol FF

• Used as a tracking dye at 5kb to monitor the progress of electrophoresis separation
• CAS Number: 2650-17-1
• DNase/RNase/Protease free

IB74040
Bromophenol Blue

• Tracking dye in electrophoretic separations
• Migrates around 0.5kb
• CAS Number: 115-39-9
• Purity: >98.0%

RunBlue SDS Running Buffer (NXB50500) a TEO-Tricine buffer system which provides a separation similar to the MOPS buffer used with BIS-TRIS precast gels. It provides enhanced separation of higher molecular weight proteins. The buffer can be for reduced and non-reduced samples.

RunBlue TGS Blot Buffer(NXB82600) has been specially formulated to provide optimal blot transfer of proteins from RunBlue Precast Gels. Supplied as a concentrate should be diluted by a factor of 10 for use with the RunBlue Dual Run and Blot system or a factor of 20 for use with other blotting methods.