PR1MA™ Rapid Coomassie Stain

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PR1MA™ Protein Gel-Loading Dye for SDS-PAGE

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Hoefer® Mighty Small Transfer Tank

The TE22 Mighty Small Transfer Tank quickly and efficiently transfers proteins and nucleic acids from small gels to nylon, nitrocellulose or PVDF membranes. Maximum Gel Size 9 x 10 cm.

• Transfer as many as four small gels, up to 9 x 10 cm in less than an hour
• Uniform and strong electric field—supports efficient and even transfers
• Color-coded, easy to assemble and load cassettes—ensures proper orientation during transfer
• Superior tank design allows the cassettes to apply equal pressure across the stack - prevents gel distortion
• Built-in ceramic heat exchanger—provides excellent temperature control when used with an external cooling water bath
• Built-in alumina-covered cooling channel provides excellent temperature control with no more than a 5°C temperature increase during a typical run

Hoefer® Mighty Small Transfer Tank Includes

• Lower Chamber with Heat Exchanger, 1/ea
• Safety Lid with High Voltage Leads, 1/ea
• Electrode Panels, 2/pk
• Cassettes, 4/pk
• Foam Sponges, 6 mm thick, 4/pk
• Foam Sponges, 3 mm thick, 8/pk
• Cassette Hook, 1/ea
• Blotter Paper (9 x 10.5 cm), 25 sheets/pk

Hoefer® Mini Electrophoresis and Western Blot Package

Specifications
 

Capacity	Up to four 9 x 10 cm gels
Max. Power	100 V, 500 mA, 50 W
Max. Temp.	113ºF | 45°C
Indoor Use	39.2 to 104ºF | 4 to 40°C
Humidity	Up to 80%
Dims (D x W x H)	6.50 x 5.51 x 9.45 In. | 16.5 x 14 x 24 cm
Weight	4.441 lb | 2.00 kg

Hoefer® Standard Transfer Tank

The TE42 Standard Transfer Tank quickly and evenly transfers proteins and nucleic acids from polyacrylamide or agarose gels onto nylon, nitrocellulose, or PVDF membranes. The four slot design of the TE42 enables either cooled or non-cooled protein or nucleic acid transfers. The optional heat exchange module is easily incorporated into the TE42 enabling transfer of up to 2 gels simultaneously. When used without the optional heat exchange module, up to four gels can be transferred at one time.

• Uniform and strong electric field—supports efficient and even transfers
• Color-coded, easy to assemble cassettes—ensures proper orientation during transfer
• Superior tank design allows the cassettes to apply equal pressure across the stack - prevents gel distortion
• Optional TE47 heat exchanger—provides excellent buffer temperature control when used with an external cooling water bath
• Heat exchanger from an SE600 series electrophoresis unit can be substituted for the TE47 heat exchanger. 

Hoefer® Standard Transfer Tank Includes

• Lower Chamber, 1/ea
• Safety Lid with High Voltage Leads, 1/ea
• Electrode Panels, 2/pk
• Cassettes, 2/pk
• Foam Sponges, 6 mm thick, 2/pk
• Foam Sponges, 3 mm thick, 4/pk
• Cassette Hook, 1/ea
• Blotter Paper (14.5 x 21.5 cm), 25 sheets/pk

Specifications
 

Capacity	Up to four 15 x 21 cm gels (w/out cooling) Up to two 15 x 21 cm gels (w/ cooling)
Max. Power	100 V, 2 A, 200 W
Max. Temp.	113ºF | 45°C
Indoor Use	39.2 to 104ºF | 4 to 40°C
Humidity	Up to 80%
Dims (D x W x H)	12.01 x 3.15 x 5.12 In. | 30.5 x 8 x 13 cm

Hoefer® Standard Transfer Tank with Cooling Chamber

TE62 Standard Transfer Tank with Cooling Chamber quickly and evenly transfers proteins and nucleic acids from polyacrylamide or agarose gels onto nylon, nitrocellulose, or PVDF membranes while providing excellent temperature control.

The TE62 is designed to accept up to four cassettes simultaneously with cooling. Each cassette holds a 15 x 21 cm gel, or as many as four 7 x 10 cm mini gels.

• Uniform and strong electric field—supports efficient and even transfers
• Color-coded, easy to assemble cassettes - ensures proper orientation during transfer
• Superior tank design allows the cassettes to apply equal pressure across the stack - prevents gel distortion
• Built-in ceramic heat exchanger - provides excellent temperature control when used with an external cooling water bath  

Hoefer® Standard Transfer Tank with Cooling Chamber Includes

• Lower Chamber with Heat Exchanger, 1/ea
• Safety Lid with High Voltage Leads, 1/ea
• Electrode Panels, 2/pk
• Cassettes, 4/pk
• Foam Sponges, 6 mm thick, 4/pk
• Foam Sponges, 3 mm thick, 8/pk
• Cassette Hook, 1/ea
• Blotter Paper (14.5 x 21.5 cm), 25 sheets/pk 

Specifications
 

Capacity	Up to four 15 x 21 cm gels Up to sixteen 7 x 10 cm gels
Max. Power	100 V, 2 A, 200 W
Max. Temp.	113ºF | 45°C
Indoor Use	39.2 to 104ºF | 4 to 40°C
Humidity	Up to 80%
Dims (D x W x H)	12.60 x 11.02 x 6.50 In. | 32 x 28 x 16.5 cm

Gel Crusher™ Spin Column

Rapid Gel Fragmentation for DNA Recovery.

Gel Crusher™ Spin Columns provide a fast, efficient, and consistent method for gel fragmentation, replacing traditional crush-and-soak or manual gel breaker steps. Designed with 600 uM filtration pores, these columns enable quick and uniform fragmentation of agarose or acrylamide gel slices, saving time and increasing DNA recovery yields.

Ideal for workflows in molecular cloning, PCR cleanup, restriction digests, DNA sequencing, and labeling protocols, the Gel Crusher™ simplifies the preparation of gel fragments for downstream purification and elution steps.

Simply insert the Gel Crusher™ column into a 2 mL microcentrifuge tube, add your pre-cut gel slice (prepared using a gel cutter or razor blade), and centrifuge at max speed (~15,000 x g) for 5 minutes. The gel will be forced through the 600 uM pores, producing small, uniform fragments that can be easily pipetted using wide-bore tips into NaCl or other solutions.

This process creates a fine slurry that enhances elution efficiency and improves access to the target nucleic acids, significantly reducing incubation and processing time.

Key Features

• Efficiently breaks gel slices into uniform fragments
• Eliminates manual crush-and-soak steps
• Increases DNA yield and recovery efficiency
• Fits standard 2 mL microcentrifuge tubes
• Compatible with standard benchtop centrifuges (up to 15,000 x g)
• 600 uM pore size ideal for agarose or acrylamide gels
• Simplifies downstream processes: elution, filtration, incubation
• For use in PCR, cloning, digestion, sequencing, and more 

Reversible Racks

• Choose 60 well (5 x 12) or 96 well (8 x 12) formats
• Reversible racks, accept 0.5 and 1.5 to 2.0 mL microtubes
• Alphanumeric marking on each well
• Made of polypropylene

Azure HRP Stripping Buffer

Works by removing the primary and secondary antibodies from the membrane

Stripping buffer for stripping and re-probing chemiluminescent Western blots.

• Provided as a 1x ready-to-use solution
• Size: 500mL

Azure Aqua Transfer Cell

Perform a quick 1 hour transfer or transfer overnight at a lower voltage

Aqua Transfer Cell is used for transferring two mini gels to membranes for Western blotting experiments (wet transfer). The transfer cassettes and electrode core are colored for directionality which makes transferring easy and straightforward.

Includes:

• 2 transfer cassettes
• 6 sponges
• 2 Azure Aqua cooling units
• Tank and upper lid with power cables
• Transfer Electrode Cores

Features

The transfer cassettes and electrode core are colored for directionality, making transferring easy and straightforward.

Chemical reagent compatibility

Keep all components of the Azure Aqua Transfer Cell away from chloroform, arenes (such as methylbenzene benzene), and acetone. Any damage caused by these reagents is not covered by the warranty policy.

 
Cleaning Instructions

Before using, wash all components with a neutral detergent and rinse well with ultra pure water.

Wash the electrode assembly, transfer cassettes, and buffer tank with a neutral detergent and warm water. Be careful to avoid damaging the platinum electrodes. Use warm water to rinse the sponges, and then rinse all components with distilled or ultra-pure water.

Specifications
 

Azure Transfer Buffer

This proprietary transfer buffer provides improved detection of low-abundance and post-translationally modified proteins.

High-efficiency protein transfer and increased protein retention on the membrane add up to more sensitive Western blots.

• Provided as a 50x concentrated solution. Sufficient for 50-100 blots
• Size: 500mL

Features

• High performance—increased protein retention on the membrane and fast transfer times result in more sensitive detection of low-abundance and post-translationally modified proteins
• Efficient—enhanced transfer efficiency for proteins of all sizes
• Fast—transfer proteins in less than 20 minutes vs. several hours required with traditional transfer buffers
• Convenient—use your existing wet transfer electrophoresis apparatus

Azure Chemi Blot Blocking Buffer

This Chemi Blot Blocking Buffer was formulated to enhance signal and lower background by improving sensitivity. Enhances specific antibody-antigen interactions specifically for chemiluminescent Western blots. 

• Provided as a 1x ready-to-use solution- no mixing or dilutions required.
• Size: 500mL

Features

Western blot membranes should be incubated in blocking buffer before antibody hybridization to prevent non-specific binding of antibodies. Blocking buffer can also be used for diluting primary and secondary antibodies during hybridization.

Azure Blot Washing Buffer

Wash buffers are an important part of the blotting process for removing unhybridized antibody and preventing background signal.

Blot washing buffer for use with Azure Imaging Systems, or any other Western blotting system.

• Provided as a 10x concentrated solution
• Size: 500mL

NOTE: While this Blot Washing Buffer is compatible with NIR Fluorescent Western blots, for the best results with NIR fluorescence, we recommend the Azure Fluorescent Blot Washing Buffer.

Azure Protein-free Blot Blocking Buffer

Protein-free blocking solution optimized for fluorescent Immunoblots and Western blots

Does not contain proteins. Azure Protein-Free Blot Blocking Buffer can reduce background in Western blots where the primary antibodies tend to cross-react with protein blockers like BSA, casein or milk protein. For low-quality primary antibodies that may require protein-based blocking agents, BSA or non-fat dry milk can be dissolved directly in the Azure Protein-Free Blot Blocking Buffer and then used to dilute the antibody.

Best used with assays where animal-based product usage is prohibited. Serves as an excellent blocking reagent for chemiluminescent Western blotting.

• Provided as a 5x solution
• Size: 200mL