S2K Commerce - Products Dropdown
Actions
S2K Commerce - Shopping Cart
Actions
S2K Commerce - Order Entry
Actions
PR1MA™ Protein Gel-Loading Dye for SDS-PAGE
- Appearance: Slightly Viscous Dark Purple Liquid
- DNase, RNase, protease: Not Detected
- Electrophoresis: Pass Test
- OPTICAL ABS AT 525 NM: 0.15 - 0.35
- OPTICAL ABS BETWEEN 588-594 NM: 0.32 - 0.47
- OPTICAL ABS BETWEEN 635-641 NM: 0.25 - 0.40
Proudly made in the USA! Click here for more made in America products!
Protein, Gel-Loading, Dye, SDS-PAGE,PR1MA, MIDSCI, Laboratory Supplies, High-Quality, Research, Consumables, Reagents, Equipment, Customer Service, Quick Shipping, Satisfaction Guarantee, Personalized Solutions, Universities
Azure HRP Stripping Buffer
Works by removing the primary and secondary antibodies from the membrane
Stripping buffer for stripping and re-probing chemiluminescent Western blots.
- Provided as a 1x ready-to-use solution
- Size: 500mL
Azure Transfer Buffer
This proprietary transfer buffer provides improved detection of low-abundance and post-translationally modified proteins.
High-efficiency protein transfer and increased protein retention on the membrane add up to more sensitive Western blots.
- Provided as a 50x concentrated solution. Sufficient for 50-100 blots
- Size: 500mL
Features
- High performance—increased protein retention on the membrane and fast transfer times result in more sensitive detection of low-abundance and post-translationally modified proteins
- Efficient—enhanced transfer efficiency for proteins of all sizes
- Fast—transfer proteins in less than 20 minutes vs. several hours required with traditional transfer buffers
- Convenient—use your existing wet transfer electrophoresis apparatus
Azure Chemi Blot Blocking Buffer
This Chemi Blot Blocking Buffer was formulated to enhance signal and lower background by improving sensitivity. Enhances specific antibody-antigen interactions specifically for chemiluminescent Western blots.
- Provided as a 1x ready-to-use solution- no mixing or dilutions required.
- Size: 500mL
Features
Western blot membranes should be incubated in blocking buffer before antibody hybridization to prevent non-specific binding of antibodies. Blocking buffer can also be used for diluting primary and secondary antibodies during hybridization.
Azure Blot Washing Buffer
Wash buffers are an important part of the blotting process for removing unhybridized antibody and preventing background signal.
Blot washing buffer for use with Azure Imaging Systems, or any other Western blotting system.
- Provided as a 10x concentrated solution
- Size: 500mL
NOTE: While this Blot Washing Buffer is compatible with NIR Fluorescent Western blots, for the best results with NIR fluorescence, we recommend the Azure Fluorescent Blot Washing Buffer.
Azure Protein-free Blot Blocking Buffer
Protein-free blocking solution optimized for fluorescent Immunoblots and Western blots
Does not contain proteins. Azure Protein-Free Blot Blocking Buffer can reduce background in Western blots where the primary antibodies tend to cross-react with protein blockers like BSA, casein or milk protein. For low-quality primary antibodies that may require protein-based blocking agents, BSA or non-fat dry milk can be dissolved directly in the Azure Protein-Free Blot Blocking Buffer and then used to dilute the antibody.
Best used with assays where animal-based product usage is prohibited. Serves as an excellent blocking reagent for chemiluminescent Western blotting.
- Provided as a 5x solution
- Size: 200mL
Azure Western Blot Quenching Sheets
Background quenching sheets for use with fluorescent or chemiluminescent samples
Use these quenching sheets under your blot when imaging fluorescent or chemiluminescent Western blots. Sheets reduce background autofluorescence or background signal from contamination in shared imagers, improving the quality of your image.
- For use with fluorescent or chemiluminescent samples.
- Size: 9 x 12.5 cm
- Quantity: 10 Sheets
Features
Use these quenching sheets under your blot when imaging fluorescent or chemiluminescent Western blots.
The sheets reduce background autofluorescence or background signal from contamination in shared imagers, improving the overall quality of your image.
Azure Flash Western Chemiluminescence Kit
Kit includes all reagents necessary for performing ten chemiluminescent Western blots using the Flash Western protocol
Each kit includes:
- 10x PVDF Membranes
- 100mL Chemi Blot Blocking Buffer
- 500μL Flash HRP Conjugate
- 250mL Flash Wash Buffer
- 100mL Radiance ECL Substrate
Features
Time savings using Azure Flash Western Chemiluminescent Kit. Flash Western graph includes use of Azure Transfer Buffer in light blue.
The Azure Flash Western Kit uses a proprietary set of reagents to achieve complete membrane blocking, rapid hybridization of antibodies, and enhancement of chemi signal in less than an hour of blotting time. The included transfer buffer cuts wet transfer time down to 15 to 30 minutes for a total assay time of two hours from gel to imaging.
- Fast protocol with few hands-on steps
- Antibody stabilization for enhanced chemi signal
- Low background and increased signal-to-noise ratios
- Increased detection sensitivity and linear dynamic range
The Rotocycler 72 facilitates the handling and insertion of caps on the tubes by offering a special solid aluminum loading rack. This rack can hold up to 18 x 0.1 mL tube strips for a total of 72 tubes. Other cavities can hold larger reaction tubes. To keep reactions cool during setup, simply place the rack in a refrigerated area. For easy reference, all wells are numbered. Color coding is made possible by inserting a Capinsert in up to two locations on the rack.
Includes: Rotocycler 72 and 500 assorted color coding inserts
Accessories for the Rotocycler 72
T319-4N |
Rotocycler 0.1 mL Tube and Cap Strips for Qiagen Rotor-Gene Q |
T319-72D2 |
Rotocycler 72 Rotor with 100 µL wells for Qiagen Rotor-Gene Q |
T319-100D1 |
Rotocycler 100 Rotor with 25 µL wells for Qiagen Rotor-Gene Q |
The RotoCycler Discs are specially made to be used with the Qiagen Rotor-Gene Q Real-Time Rotary Analyzer. Two models are available: a 72-well format with 100 µL tubes and a 100-well format for reactions up to 25 µL. The discs a"one-piece "plate" equivalent, having vertically oriented wells compatible with automated reaction setup using a robotic liquid handling system.
- The Extractor removes over 99% of the ethidium bromide from gel staining solutions quickly and easily.
- Each device can decontaminate up to 10 liters of gel staining solution
The RotoCycler Discs are specially made to be used with the Qiagen Rotor-Gene Q Real-Time Rotary Analyzer. Two models are available: a 72-well format with 100 µL tubes and a 100-well format for reactions up to 25 µL. The discs a"one-piece "plate" equivalent, having vertically oriented wells compatible with automated reaction setup using a robotic liquid handling system.
Web Content Viewer
Actions