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Liquid Handling Instruments

PCR Purification Tips

DIFFINITY RAPID TIP

Diffinity RapidTip® - PCR clean up in a tip in 60 seconds!

Purification of PCR products for Sanger Sequencing in just one minute! No bind-wash-elute, enzymes, or magnetic beads necessary! An innovative new technology that removes undesirable impurities from PCR reactions leaving you with nothing but purified DNA for your Sanger sequencing applications. Primers and dNTPs will be gone in 60 seconds!

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Key Features:

  • Diffinity RapidTips®- pre-packed with our proprietary material and ready to use out of the box!
  • A standard pipettor - single or multi-channel.
  • Microcentrifuge tubes to store purified DNA.
  • 20-30µl PCR reaction volumes
 

Application:

Purified PCR product for Sanger sequencing applications. The tip attracts and retains dNTPs, primers and primer dimers of < 50bp and repels and permits the double stranded DNA targets (such as PCR amplicons) to remain in solution. Features:

 

Excellent Yield
Recovers up to 90% of high quality dsDNA ready to use in subsequent applications.

One Minute, One Step
Extremely fast and efficient process to rapidly recover clean DNA.

Cost Effective
No capital equipment (e.g., centrifuge, magnetic extractor, vacuum manifold), extra plasticware, or liquids are required. Less labor time required due to simple protocol.

Robot Compatible
Easily integrated on automated equipment for high throughput processing and increased productivity. Same purification process, eliminates additional process validation.

No Bind-Wash-Elute Steps
Eliminates use of large amounts of reagents and time-consuming, tedious protocols.

Effective Purification
Removes up to 90% of primers, ssDNA, and primer dimers.

Large Range of Fragment Length
Returns pure DNA fragments of 100bp to 10Kb. Use 20-30µl PCR reaction volumes

Easy to Use
Single step protocol requires little to no training of lab personnel. Simple protocol requires no complex operator techniques or interactions with equipment, buffers, or reagents.

Environmentally Friendly
Waste is limited to the functional tip. No extra materials, tips, columns, buffers, or reagents required to purify PCR product.

Seamless Workflow
Uses standard pipettor so no changes are needed to your current lab workflows.

How It Works

The functional pipette tip will fit on most conventional single or multiple, manual or automated laboratory pipettors. Samples containing DNA and impurities are aspirated into the tip where the impurities are adsorbed onto proprietary surfaces within the tip by mixing for approximately one minute. Expelling the solution yields purified DNA, the desired product.

 

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Frequently Asked Question

Item Description
RT025-048 Diffinity RapidTip (48) for PCR Purification (1 Box of 48 tips)
RT025-096 Diffinity RapidTip (96) for PCR Purification (1 Box of 96 tips)

 

 

Pipette tips from MIDSCI offer maximum sample volume recovery in aspiration and dispension applications. Rely on MIDSCI for quality low-binding and maximum recovery pipette tips from popular brands including AvantGuard.

AvantGuard tips have a:

  • Unique resin infused polypropylene tips for superior low-binding technology
  • Polyethylene barrier for pipettor contamination prevention
  • Extended lengths available
  • Both hinged and lift-off lids available
  • No leaching or sample degradation
  • Safe to use with chloroform, chloroethane, acetone, and other common laboratory solvents