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Brilliant Calcium Kits

High-throughput, no wash calcium (Ca²+) assay. An optimal solution for measuring intracellular Ca²+ dynamics, including effectors such as GPCRs and protein transporters.

ION Biosciences’ Brilliant Calcium (Ca²+) Assay is a total assay solution for multi-well plate-based, high-throughput measurements of dynamic changes in intracellular Ca²+. The ION Biosciences’ Brilliant Calcium Assay is useful for a wide range of effectors of intracellular Ca²+ including many G protein-coupled receptors (GPCRs), divalent cation channels, and other proteins capable of transporting Ca²+ or affecting intracellular Ca²+ dynamics. In multi-well, plate-based formats, the Brilliant Calcium Assay can be used to discover and characterize the effects of tens to many tens-of-thousands of compounds and environmental factors which can act to affect intracellular Ca²+ levels.

Brilliant Calcium Flex Kit

Our Flex kit provides all necessary reagents in a format to maximize flexibility for your assay design and customization challenges.


Contents of Brilliant Calcium Flex Kit

 

Label

Name

Volume

Containers

Storage

Reagent A

Brilliant Calcium Reagent

Dry

10

-20° C

Reagent B

DMSO

225 μL

1

4° C

Reagent C

DySolv

4 mL

1

4° C

Reagent D

10X Assay Buffer

20 mL

1

4° C

Reagent E

TRS

4 mL

1

4° C

Reagent F

Probenecid Solution

4 mL

1

4° C

  

Brilliant Calcium Express Kit  

Our Express kit is custom tailored to customer’s needs, providing only the reagents you need in the amounts you need them. Less waste, higher reproducibility, and more convenience.
  

*Research or further manufacturing use only, not for food or drug use.

ION Biosciences Brilliant Sodium Flex Kit
 
ION’s Brilliant Sodium Assay is a total assay solution for multi-well plate-based, high-throughput measurements of changes in intracellular Na+ mediated through a wide-variety of plasma membrane and intracellular sodium channels and transporters. In multi-well, plate-based formats, the Brilliant Sodium Assay can be used to discover and characterize the effects of many tens-of-thousands of compounds and environmental factors on effectors of intracellular Na+. ION’s Brilliant Sodium Assay provides all the reagents necessary for use as a wash or no-wash assay with adherent or non-adherent cells. The optional use of a probenecid solution and an extracellular background masking solution (TRS) offers the ultimate in compatibility for cells types which are difficult to load with fluorescent Na+ indicators (e.g. Chinese Hamster Ovary, CHO cells) and when performing assays in complete, serum-containing cell culture medium is desired.
 
ION’s Brilliant Sodium Assay is compatible with fluorescence microscopes, flow cytometers, and plate readers capable of detecting fluorescein or more optimally, yellow fluorescent protein (YFP).
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