PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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The SmartBlue™ Blue Light Transilluminator 

 
The SmartBlue™ Blue Light Transilluminator is the smart alternative to UV transillumination. The uniform 465 nm bottom lighting illumination is perfect for excitation of most green fluorophores without damaging the DNA, thus resulting in a much higher downstream cloning efficiency. Furthermore, the two position filter cover position allows for easy access to the gel for band excision. Without the need for the use of DNA damaging UV light, you don't have to worry about any damage to the skin and eyes.


The LEDS are long lasting (with a 30,000 hour service life) providing a durable and efficient illumination option. Gels can be excised directly on the unit with the scratch resistant surface. The unit contains an automatic 5 minute timed shut off for energy saving as well. The cover can be placed flush with the unit, at a 60 degree angle, or completely removed for use with the SmartDoc Imager (E5000-SD).

Pair with DNA Safe Stain (C138) for the ultimate viewing effectiveness!

A 230V is available upon request (SB4500-E).

Specifications

Light Source: High Intensity Blue LEDs
Emission Filter Cover: Amber
Wavelength: Peak at 465 nm
E4000 Viewing Surface: 17 x 12 cm
E4000 Ext. Dimensions 30.5 x 21.5 x 5 cm
E4100 Viewing Surface 10.5 x 10.5 cm
E4100 Ext. Dimensions 17 x 12 x 5 cm
Electrical: 100 to  240V, 50-60Hz
Weight: 1.2kg / 2.6lbs
Warranty: 2 years



SmartBlue™ Mini Blue Light Transilluminator

Safer for samples, safer for you: Accuris SmartBlue™ Mini Blue Light Transilluminator is the perfect partner for most common safe DNA stains. It emits blue light at a wavelength of 465nm, perfect for excitation of most green fluorophors, including our Accuris’ SmartGlow™, SYBR Green, SYBR Safe, SYBR Gold, GelStar, GelGreen, to name a few.  The SmartBlue Mini also works for excitation of EtBr (though fluorescence is not as bright as with UV), see our Technical Bulletin with sample images. Exposing DNA to blue wavelength does not cause molecular damage, and results in a much higher downstream cloning efficiency as compared to even short UV light exposures.  In addition, the visible blue light is not damaging to the user’s skin and is less harmful to the eyes.*


Two position filter cover:  The E4100 transilluminator comes with an emission filter cover. These amber covers, which rest above the viewing surface and gel, filter out the blue light, allowing the fluorescing DNA bands to be seen clearly.  The covers are designed to fit flat for general gel viewing or to be placed at an angle to provide easy access to the gel for band excision. For documentation, the filter cover is easily removed completely. UI technology, bright and clear images:  The bright LED light plates and filter layer provides an extremely evenly lit viewing surface. Even in ambient light, bands of DNA can clearly be seen in stained gels. Images obtained using the SmartBlue Mini Transilluminator are brighter and more balanced than those obtained using epi-illuminators which light from the sides.


Reliable, durable and long lasting:  An array of super bright LEDs with a long, 30,000 hour service life provide the light source for the SmartBlue™ Mini Transilluminator. Unlike those in a UV transilluminator, the filters in the SmartBlue Min system will not solarize and degrade in performance over time. Gel bands can be excised directly on the scratch resistant, glass viewing surfaces. To save energy, the power switch includes an automatic 5 minute shutoff. SmartBlue™ Mini Transilluminators are covered by a 2 year warranty.


* The SmartBlue™ products use high intensity LED lights.  Although the visible blue wavelength is significantly safer than UV light, it is recommended to not stare directly at the viewing surface without the amber cover in place. 
 
 
Specifications 

Light Source

High Intensity LEDs

Filter Cover

Amber, for filtering of blue light

Exterior Dimensions

6.7 x 4.7 x 1.9 in | 17 x 12 x 5 cm

Electrical

100-240 V, 50/60 Hz

Output Wavelength

Peak at 465 nm

Viewing Surface

3.9 x 4.1 in | 10 x 10.5 cm

Weight

0.2 kg | 0.5 lb

Warranty

2 years



Optional SmartBlue Plus Viewing Glasses


Accuris SmartBlue™ Plus Viewing Glasses offer exceptional protection for researchers working with bright blue LED and UV light illuminators. The polycarbonate filter lenses are shatter proof, scratch resistant and block light in the UV (280 nm) to blue (490 nm) wavelengths. In addition, they are compliant with ANSI Z87.1.

 

These glasses are ideal for general protection when working with hazardous wavelengths and for precision DNA band cutting on a UV or blue light transilluminator. While blocking hazardous UV and blue light, the glasses allow clear visualization of DNA bands stained with Ethidium Bromide, SmartGlow™, SYBR Green and other fluorescent dyes. 
 
*Always wear appropriate UV protection for eyes and exposed skin when working with UV light sources 

 

Item#:
ASSMARTBLUELGT

Double sided water cooled vertical system, 20cmW x 10cm


The Galileo Bioscience Reflection 2010 is a wide, high capacity gel electrophoresis separation system that allows users to run up to two protein gels simultaneously on a 20 cm wide x 10 cm tall format.  The large gel format makes this unit ideal for users who have a large number of protein samples to run simultaneously.  Galileo's unique casting base allows users to both cast and run gels without ever having to unclamp the upper buffer chamber.  This makes handling gels safer and more convenient, especially for those who may be prone to ripping their gels between the two activities. 

The 2010 vertical gel box also includes a water cooling feature for electrophoresis protocols that require lower temperature.  This water can be provided by the tap or other temperature controlled source and is easily connected by attaching standard tubing over the 3/8" barbed in/out ports on the device.  Users may also facilitate cooling efficiency and uniformity using Galileo's optional alumina backer plates (see accessories below).

System Features

Each Reflection 85-2010-V vertical system includes the following features:

  • Heavy duty upper buffer chamber with cooling port connections
  • Lower buffer chamber
  • Interlock safety lid with attached leads
  • Casting base
  • Four-pack each of plain and notched glass plates
  • One acrylic blocker plate (used when only one gel is being run)
  • Eight 0.8mm thick side spacers
  • Four combs of your choice
  • System Accessories

Alumina cooling plate for use with cooling systems. This plate can be sandwiched between the gel and the inner buffer chamber to improve overall temperature uniformity.
 
Casting base for quick, efficient and leak-proof gel casting.
Item#:
ASDOUBLESIDEWATVER20
  • Increased ultraviolet output and intensity
  • No light flicker
  • Wider intensity range with variable selection (select models)
  • Fast lamp start up
  • Universal power supply
  • Reduced electrical consumption
  • Longer tube life
  • Lighter weight
  • Improved gel visualization and gel documentation
  • 2 year warranty

Visi-Blue Transilluminators are designed for use with GFP stains. As compared to UV, the blue light will transmit through almost any transparent (plastic, glass, etc.) laboratory equipment. This is essential when monitoring and detecting DNA migration and protein expression in real time.

  • Two models are available with surface areas as follows: 21x26cm or 20x40cm
  • Select from models with 8-watts or 25-watts
  • Amber cover (included) blocks blue light transmission and allows visualization of media above 500nm
Item#:
ASPCRUVEQ10

Several specially designed converter plates convert a transilluminator's 302nm ultraviolet radiation .

  • To white light for viewing protein gels, coomassie blue stained or silver stained media. The Converter Plate's uniquely phosphored glass assembly (patent pending) converts the UV radiation via a white diffuser
  • Convert UV:
  1. To 460-470nm with the Visi-BlueT plate for viewing GFP stains
  2. To 365nm UV with the UV/UV plate for preparation and gel excision
  • The scratch-resistant glass is mounted in a metal housing for durability
  • Rubber seal stops the plate from sliding
  • Handles are situated on two sides of the plate for easy handling
  • Several standard plate sizes are available; custom sizes are also available
Item#:
ASPCRUVEQ15

ENDURO Power Supply 

  • Two models (300V and 250V) available
  • For DNA, RNA, or protein electrophoresis, and blotting applications
  • Constant voltage or constant current with automatic crossover
  • 250V model also offers a constant power operating mode
  • Timed or continuous operation
  • Short circuit and over current/ over voltage protection
  • No load and load change detection system
  • Programming includes option for automatic recovery after power failure
  • 3 year warranty

The new Enduro Power Supplies meet a wide range of applications including DNA, RNA, and protein electrophoresis, blotting, and many others. Two models are available with voltage ranges to 300V and current ranges to 3000mA. Operating modes include constant voltage or constant current with automatic crossover. In addition, the 250V model offers a constant power operating mode.

All parameters, including voltage, current, power (250V model only), and time are easily set using the membrane keypad and displayed digitally on the back lit LCD. Compact in design with a small footprint, the Enduro Power Supplies are also stackable to save space. For easy viewing and setting of parameters on the bench or on a shelf, the front of the power supply can be raised with the integral folding feet.
 

Specifications 

Model

300V

250V

Output Voltage Range/Increments

2-300V/1V

5-250V/1V

Output Current Range/Increments

4-500mA/1mA

10-3000mA/10mA

Output Power Range/Increments

90 watts max/n.a.

1-300W/1W

Operating Constant Modes

current or voltage

current, voltage, or power

Timer

1 -99 min, or continuous

1min. to 99h 59min or continuous

Programmable

no

Yes (20 program capacity)

Dimensions (W x D x H)

19 x 25 x 8 cm

19 x 25 x 8 cm

Weight

2.6 lbs

5.7 lbs

Input Voltage

110-230 V

110-230 V

Item#:
ASPOWERSUP1
Please know that the manufacturer has suspended manufacturing this product indefinitely. 
We suggest purchasing PR1MA-MyVolt-Touch.
Contact your rep or email tech@midsci.com for more information.


The ENDURO Mini is a great saver of space and money. This power supply can handle up to 2 gel boxes at atime and is great for horizontal DNA gels and mini-vertical protein gels. These are small in size but not performance. The ergonomic design of the ENDURO Mini allows them to be stacked. However, to minimize cabling, up to three power supplies can be placed within the PowerStock allowing optimal air circulation while only utilizing one power outlet at the bench. These units are full featured with 300V, 400mA, 60W and built in timer. They can run at either constant voltage or constant current and have automatic cross-over as well as no-load detection.


Output voltage/increments

10-300V/ 1V

Output current/increments

10-400mA/1mA

Output power

60W

Operating constant modes

Constant voltage or constant current

Timer

1-999 minutes with alarm, or continuous

Dimensions (WXDXH)

140 X 191 X 84 mm

Weight

1 kg

Input voltage

100-240V

Item#:
ASPOWERSUP2
Please know that the manufacturer has suspended manufacturing this product indefinitely. 
We suggest purchasing PR1MA-MyVolt-Touch.
Contact your rep or email tech@midsci.com for more information.



The ENDURO Mini is a great saver of space and money. This power supply can handle up to 2 gel boxes at atime and is great for horizontal DNA gels and mini-vertical protein gels. These are small in size but not performance. The ergonomic design of the ENDURO Mini allows them to be stacked. However, to minimize cabling, up to three power supplies can be placed within the PowerStock allowing optimal air circulation while only utilizing one power outlet at the bench. These units are full featured with 300V, 400mA, 60W and built in timer. They can run at either constant voltage or constant current and have automatic cross-over as well as no-load detection.


Output voltage/increments

10-300V/ 1V

Output current/increments

10-400mA/1mA

Output power

60W

Operating constant modes

Constant voltage or constant current

Timer

1-999 minutes with alarm, or continuous

Dimensions (WXDXH)

140 X 191 X 84 mm

Weight

1 kg

Input voltage

100-240V

Item#:
ASPOWERSUP3

Need a great (q)PCR Regents at a great price? Try PR1MA!  Shop GreenGoldProbe, or One Step.


New and Improved! Validated for SNP Genotyping assays, Gene expression assays, Microarray validation and High throughput screening!

Bullseye EvaGreen qPCR MasterMix is designed for quantitative/real-time analysis. The components of Bullseye EvaGreen qPCR MasterMix has been developed for superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. The chemically modified Hot-Start Taq polymerase, included in our Mastermix, significantly reduces non-specific PCR amplification observed with regular Taq polymerase. Every lot is tested on different real-time PCR machines listed below.

  • Instrument-specific & pre-optimized
  • Validated applications
  • Reproducible results
  • Highly competitive pricing


For laboratory research only. Not for clinical applications.

For technical questions, phone the MIDSCI helpline at 800-227-9997

Item#:
ASQPCR1

These compact power supplies cover a wide range of requirements for electrophoretic separations. The SH-500 is ideal for DNA or RNA submarine gels, PAGE & SDS-PAGE, multiple horizontal gels and mini blotting applications. It features a built-in timer, constant voltage or constant current, and a unique Gel Saver mode (1mA output to prevent diffusion after the timer expires). The easy-to-use SH-3000 offers constant voltage, current and power, making it perfect for DNA sequencing systems and SDS-PAGE applications. 4 year warranty

                                    
Model: SH-3000
Voltage: 10-3000V, 10V steps
Max. Current: 300mA, 1mA steps
Power: 300 watts
Fault Detection:Stop & Audible Alarm
Operating Temperature:0-40°C
Dimensions (W x D x H):27 x 28 x 11cm
Weight: 4kg
Item#:
SH-3000
Your Price:
3487.71
Each

This product has been discontinued.

Please visit the New PR1MA™ qMAX Green qPCR Mix with Blue Tracking Dye for more options!

 

 

PR1MA™ qMAX™ Gold

  • Inert yellow dye helps reduce pipetting errors
  • Room temperature stable for up to 30 days
  • Compatible with fast cycling protocols
  • Highly sensitive for low copy number templates
  • Includes PR1MA Hot Start Taq Polymerase

Successful PCR requires careful control of many variables.  Pipetting errors, poor performing polymerases, dNTP concentrations, are just a few of the variables that can all contribute to reaction problems.  PR1MA has developed their new qMAX™ Gold to control these variables and help you achieve the best possible amplification performance. To reduce the chance of pipetting errors, qMAX™ Gold includes an inert yellow dye so small volumes are easy to visualize in PCR plates.  

qMAX™ Gold is a ready to use, 2X mastermix of PR1MA Hot Start Taq enzyme, dNTPs, a sensitive fluorescent intercalating dye, and optimized reaction buffer.  The Hot Start Taq allows reaction set up at room temperature while the temperature stable formulation guarantees optimal performance even if the mix is left at room temperature for extended periods.

Just add primers and DNA targets to the mix, and then proceed to amplification.  qMAX™ Gold is compatible with all real time thermal cyclers and exhibits high sensitivity with normal or fast 2-step cycling protocols.
 *Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.
 
 

254/302/365nm
  • Selection of each wavelength is by an easy turn of the dial located on one end of the housing
  • The uniquely designed reflector surfaces mounted behind each tube provides maximum UV for fluorescence applications
  • Lightweight and ergonomically designed for hand or stationary use
  • 1 year warranty

Dimensions
3UV-34: 9.5L x 3W x 4.5D in. (241 x 76 x 114mm)
3UV-36: 12.5L x 3W x 4.5D in. (318 x 76 x 114mm) 
3UV-38: 15.5L x 3W x 4.5D in. (394 x 76 x 114mm)

3UV Model

Part # 115V/60Hz

Watts Wavelengths
3UV-34 95-0341-01 4 Watts 254/302/365nm
3UV-36 95-0342-01 6 Watts 254/302/365nm
3UV-38 95-0343-01 8 Watts 254/302/365nm
Item#:
95-0343-01
Your Price:
1414.75
Each

Double sided non cooled vertical system, 20cmW x 10cm


 The Galileo Bioscience Reflection 2010 is a wide, high capacity gel electrophoresis separation system that allows users to run up to two protein gels simultaneously on a 20 cm wide x 10 cm tall format.  The large gel format makes this unit ideal for users who have a large number of protein samples to run simultaneously.  Galileo's unique casting base allows users to both cast and run gels without ever having to unclamp the upper buffer chamber.  This makes handling gels safer and more convenient, especially for those who may be prone to ripping their gels between the two activities.

The 2010 vertical gel box also includes a water cooling feature for electrophoresis protocols that require lower temperature.  This water can be provided by the tap or other temperature controlled source and is easily connected by attaching standard tubing over the 3/8" barbed in/out ports on the device.  Users may also facilitate cooling efficiency and uniformity using Galileo's optional alumina backer plates (see accessories below).

System Features

Each Reflection 85-2010-V vertical system includes the following features:

  •     Heavy duty upper buffer chamber with cooling port connections
  •     Lower buffer chamber
  •     Interlock safety lid with attached leads
  •     Casting base
  •     Four-pack each of plain and notched glass plates
  •     One acrylic blocker plate (used when only one gel is being run)
  •     Eight 0.8mm thick side spacers
  •     Four combs of your choice

System Accessories

  •     Alumina cooling plate for use with cooling systems. This plate can be sandwiched between the gel and the inner buffer chamber to improve overall temperature uniformity.
  •     Casting base for quick, efficient and leak-proof gel casting.
Item#:
ASDOUBLESIDENONCO20
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