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PCR/qPCR
MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more. If you are looking for any of these things, we can match almost all needs to all cyclers. If you don’t find it easily contact us at tech@midsci.com and we will find it for you.
96 or 384 we have it. Non-Skirted, semi-skirted or full skirted, we have it. 0.1, 0.2, or 0.5 mL we have it. A1, A12, H12, A24, P24 cuts, we have them. Sybr© Green or probe alternative, we have it.
Pro Tip: Colored plastics do not have any impact on DNA amplification. But when setting up qPCR it is often recommended to use white plastics. This will limit or prevent fluorescence refraction. Light refraction can minimize sensitivity and consistency.
Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips. This will help optimize and reduce variance in the reactions. Simple test, see the residual liquid left in tips? This means some of the taq, dNTPs, sample, etc is left in the tip. PR1MA tips help reduce that significantly and improve your consistency.
Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate? Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler. Call us we have solutions.
PR1MA™ PCR Strip Tubes with Attached Caps
- PR1MA™ PCR Strip Tubes and Caps are evaporation-resistant and provide easy-to-open-and-close lids that prevent sample loss.
- The uniform wells and ultra-thin walls provide optimal heat transfer and reaction efficiency.
- Reclosable bags minimize contamination risk and are convenient for opening and closing.
- For use in medical, pharmaceutical, food industry, and molecular biology.
- Certified free of detectable RNase, DNase, NA, PCR inhibitors, and tested pyrogen-free.
PR1MA™ Corbett Type PCR Tubes
- PR1MA™ PCR Strip Tubes and Caps are evaporation-resistant and provide easy-to-open-and-close lids that prevent sample loss.
- Tube Size is 0.1 mL
- 4 Tube Strip and 4 Cap Strip
- The uniform wells and ultra-thin walls provide optimal heat transfer and reaction efficiency.
- Reclosable bags minimize contamination risk and are convenient for opening and closing.
- For use in medical, pharmaceutical, food industry, and molecular biology.
- Certified free of detectable RNase, DNase, NA, PCR inhibitors, and tested pyrogen-free.
PR1MA™ Individual PCR Tubes
PR1MA™ Individual PCR Tubes
- PR1MA™ Individual PCR tubes are evaporation-resistant and provide easy-to-open-and-close lids that prevent sample loss
- The uniform wells and ultra-thin walls provide optimal heat transfer and reaction efficiency
- Reclosable bags minimize contamination risk and are convenient for opening and closing
- For use in medical, pharmaceutical, food industry, and molecular biology
- Certified free of detectable RNase, DNase, NA, PCR inhibitors, and tested pyrogen-free
- PR1MA™ PCR Strip Tubes and Caps are evaporation-resistant and provide easy-to-open-and-close lids that prevent sample loss.
- The uniform wells and ultra-thin walls provide optimal heat transfer and reaction efficiency.
- Reclosable bags minimize contamination risk and are convenient for opening and closing.
- For use in medical, pharmaceutical, food industry, and molecular biology.
- Certified free of detectable RNase, DNase, NA, PCR inhibitors, and tested pyrogen-free.
- PR1MA™ PCR Strip Tubes and Caps are evaporation-resistant and provide easy-to-open-and-close lids that prevent sample loss.
- The uniform wells and ultra-thin walls provide optimal heat transfer and reaction efficiency.
- Reclosable bags minimize contamination risk and are convenient for opening and closing.
- For use in medical, pharmaceutical, food industry, and molecular biology.
- Certified free of detectable RNase, DNase, NA, PCR inhibitors, and tested pyrogen-free.
PR1MA™ Microplate Microcentrifuge
- Quickly spins down droplets in PCR plates (less than 20 seconds)
- Accepts skirted, non-skirted, standard, and most microtiter PCR plates
- 50% smaller than traditional centrifuges
- Unique rotor design prevents sample spillage
The PR1MA™ Microplate Microcentrifuge is the first mini-sized centrifuge with a swing-out rotor for microplates. Centrifuging plates before PCR ensures all reactants are in the bottom of the wells for proper concentrations and improved yields.
The easily accessible rotor chamber includes two plate carriers that rest at a 75° angle. This allows plates to be inserted confidently without sealing tapes or caps. Upon closing the centrifuge lid, the rotor accelerates and the centrifugal force "swings" the plates into a horizontal position, concentrating any droplets on the plate walls to the well bottoms.
Specifications
| Speed | 2550 rpm / 600 xg |
| Capacity | 2 x 2 PCR Plates |
| 2 x microtiter plates | |
| (up to 25 mm) | |
| 24 x 0.2 ml PCR strips | |
| Deceleration | 4 seconds |
| Dimensions | 9.2 x 10.2 x 7.75 in. (23 x 26 x 19.7 cm) |
| Weight | 9 lbs / 4.1 kg |
| Electrical | 115V or 230V; 50-60Hz; 250W |
| Warranty | 2 Years |
Accessories
C2000-A02 - This tube adapter for the PCRCENT Plate Centrifuge holds 96 0.2 mL tubes or 8 and 12 position PCR strips. It comes 2 per pack.
C2000-DWMP - This optional rotor for the PCRCENT Plate Centrifuge has carriers for deep well plates up to 35 mm tall.
PR1MA™ qMAX Green qPCR Mix with Blue Tracking Dye
- Superior sensitivity and fast cycling with exceptional results
- Ideal for low copy number templates
- Early Ct values and detection across a broad dynamic range
- Ready to use 2X Master Mix
- Includes PR1MA™ Hot Start Taq Polymerase for greater specificity and accuracy
Supplied as a ready-to-use 2X master mix, PR1MA™ qMax Green has been engineered for high sensitivity, fast cycling, and excellent reproducibility. PR1MA™ Hot Start Polymerase provides accurate PCR of a variety of templates including low copy number and difficult sequences, while the proprietary qMax Green intercalating dye exhibits higher fluorescence and lower PCR inhibition than other popular green dyes.
These two components are supported by a specially formulated buffer with an exacting combination of salts, PCR enhancers, stabilizers, and pH that results in earlier Ct values and a high specificity across a broad dynamic range.
*Please note these products ship on dry ice. Appropriate shipping charges apply unless otherwise noted on a quote.
PR1MA™ Semi-Skirted PCR Plates
- PR1MA™ PCR Plates have uniformed wells and ultra-thin walls provide optimal heat transfer and reaction efficiency.
- Fits most thermal cyclers.
- High Contrast black alphanumerics for easy well identification.
- Reclosable bags minimize contamination risk and are convenient for opening and closing.
- For use in medical, pharmaceutical, food industry, and molecular biology.
- Barcoded versions available upon special order.
- Certified free of detectable RNase, DNase, NA, PCR inhibitors, and tested pyrogen-free
- PR-PCR2196F has a frosted surface for optimal fluorescence detection. A frosted surface reduces background noise that can interfere with quantitative real-time PCR (qPCR).
PR1MA™ TC9639 Gradient Thermal Cycler
The PR1MA™ TC9639 Thermal Cycler provides consistent, reliable results for laboratories with medium to high throughput. From its large, touch-screen user interface to its unique, multi-format block, this cycler is loaded with features. The program wizard and 10 pre-installed programs make it easier than ever to get up and running.
A large, full-color touchscreen serves as the operations center where all functions are accessed. From the “Home” screen, simply touch an icon to view the last run program, enter the program library, or use the program wizard to quickly enter a protocol. In addition, icons for favorite programs can be added for faster access.
The program wizard guides users through quickly setting up a protocol on a single screen. Once entered, programs are shown graphically and can be edited by simply touching the step that requires modification. Time and temperature increments can be built into programs as well as slower ramp rates. Storage capabilities are unlimited; programs can be stored in public folders, private (password-protected) folders, or on a flash drive. The full virtual keyboard allows for alphanumeric naming of programs and folders.The fully adjustable heated lid prevents condensation and evaporation of samples. Lid temperature can be programmed, and when the block temperature is below 35°C, the lid automatically shuts off. The heating and cooling of the block are precisely and accurately controlled within exceptionally tight tolerances by a proprietary algorithm that simulates sample temperature.
With the multi-format block, there is no need for the expense of multiple interchangeable blocks. The PR1MA™ TC9639 has the flexibility to accept 96 × 0.2 mL tubes, a 96-well plate, 0.2 mL strips or 39 x 0.5 mL tubes all in the same sample block. For protocol optimization, a gradient can be set up across the block. An optional in-situ adapter allows for the incubation of slides. Additionally, the unit may be purchased with a 384-well sample block.
Compatible with PR1MA™ and Accuris™ PCR reagents. For research use only.
Features
- Gradient capabilities- ideal for protocol optimization
- Intuitive touchscreen control- Works even when wearing gloves!
- Upgraded software- Even easier to use than before!
- No need for interchangeable blocks- multi-format accepts:
- 0.2 mL tubes or strips (96)
- 0.5 mL tubes (39)
- 96-well PCR plate
PR1MA™ Touch Power Supply
- Touch screen control
- Bright color display
- Dual orientation, auto-rotating display
The PR1MA™ Touch is a workhorse electrophoresis power supply, ideal for the efficient separation of nucleic acids and proteins as well as basic western blotting applications. Four pairs of output jacks are wired in parallel for connecting multiple electrophoresis tanks. A color touch screen allows quick setting and monitoring of all run conditions, including run time, voltage, and current. Users can select “Constant Current” or “Constant Voltage” during an electrophoresis run, keeping the chosen parameter fixed as resistance changes. The dual orientation design with a rotating display allows the PR1MA™ Touch to be installed in the most convenient location, on a shelf or bench, saving valuable space.
The PR1MA™ Touch features a logical user interface.
Setting up an electrophoresis run couldn’t be easier; simply touch the parameter to select it, press the “up” and “down” arrows to adjust the value, and then touch “Start”. All parameters are always displayed, with no need to toggle between Time, Voltage, or Amperage. In addition, the run status is always displayed.
Specifications
| Output Settings | Constant voltage or constant current |
| Voltage Output | 10 – 300 V (1 V increments) |
| Current Output | 4 – 400mA (1 mA increments) |
| Max Wattage | 100 W |
| Timer | 1 min. to 20 hours or continuous |
| Output Terminals | 4 pairs (wired in parallel) |
| Safety | No load detection, overload detection with power limitying function |
| Display | 2 x 2 in. color touchscreen |
| Dimensions | 8.5 x 6.3 x 4.0 inches |
| Weight | 2 lbs |
| Electrical* | 100 V to 230 V, 50-60 Hz (Universal Input) |
| Warranty | 2 years |
PR1MA™ Non-Skirted PCR Plates
- PR1MA™ PCR Plates have uniformed wells and ultra-thin walls provide optimal heat transfer and reaction efficiency.
- Fits most thermal cyclers.
- High Contrast black alphanumerics for easy well identification.
- Reclosable bags minimize contamination risk and are convenient for opening and closing.
- For use in medical, pharmaceutical, food industry, and molecular biology.
Certified free of detectable RNase, DNase, NA, PCR inhibitors, and tested pyrogen-free.
PR1MA™ Full-Skirted PCR Plates
- PR1MA™ PCR Plates have uniformed wells and ultra-thin walls provide optimal heat transfer and reaction efficiency.
- Fits most thermal cyclers.
- High Contrast black alphanumerics for easy well identification.
- Reclosable bags minimize contamination risk and are convenient for opening and closing.
- For use in medical, pharmaceutical, food industry, and molecular biology.
- Barcoded versions available upon special order.
- Certified free of detectable RNase, DNase, NA, PCR inhibitors, and tested pyrogen-free.
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