Implen OD600 DiluPhotometer
OD600-10 - Includes DiluCell 10, 1 Pack of 96

 
The OD600 DiluPhotometer™ is a small, easy to use instrument that is dedicated to measuring samples near the 600 nm wavelength. It is suitable for measuring growth rates of several cell types including E. coli and yeast. Use of the OD600 DiluPhotometer™ with Implen's DiluCell™ 10 facilitating the measurement of low volume samples (volume requirement of 200 µL and 100 µL respectively). The  DiluCell™ 10  allows for automatic 1/10 and 1/20 dilutions. With a bandwidth of 40 nm, the OD600 is also suitable for protein quantification assays like Bradford (595 nm). The small size and portable nature of the OD600 DiluPhotometer™ make it convenient for use in incubation cabinets and under anaerobic conditions. 

The OD600 DiluPhotometer™ has been designed for the spectrophotometric analysis of bacterial and yeast growth rates and the quantification of proteins at a wavelength nearby 600 nm (e.g. Bradford). The instrument is battery operated and can be easily transported for usage in incubation cabinets and under anaerobic conditions. The batteries last for almost 1 month when fully charged. A 600nm LED source in combination with a fiber optic is used to obtain the optical density measurement. Spills can be easily wiped from the surface, and the cell compartment area can be rinsed with, formaldehyde or ethylene oxide to sterilize it.

Lambert-Beer-Law

The Lambert-Beer-Law, also known as Beer's Law, empirically relates the absorption of light to the properties of the sample. This law states that there is a logarithmic relationship between the transmission of light through a specific sample (T = I/Io with I = outgoing light and Io = incoming light), the molar extinction coefficient for a specific compound (ε), the concentration of the absorbing species in the material (c) and the distance the light travels (d). By leaving all conditions constant but changing the pathlength (distance the light travels), you can bend the Beer-Law to get an automatic dilution of your sample. This principle is realized within the OD600 DiluPhotometer™ with DiluCell™.

Automatic Sample Dilution

Previously it was necessary to use standard cuvettes with a volume of at least 1 ml and additionally to dilute the sample manually. DiluCell™ from Implen allows for lower sample volume requirements and provides automatic virtual sample dilution. Due to the unique design of DiluCell™ cuvettes, the light-path is reduced from the standard 10 mm to 0.5 mm (DC 20) or 1.0 mm (DC 10). According to the Lambert Beer Law this shortened pathlength results in an automatic sample dilution by factor 20 (DC 20) or 10 (DC 10). Automatic dilution with DiluCell™ saves time and excludes dilution errors and cross contaminations.

Measuring Cell Density

The stage of growth of a bacterial culture needs to be monitored to ensure that the cells are harvested at the optimum point (towards the end of log phase) for the greatest density of live cells. The optical density of the sample indicates when this point has been reached. This value varies dependent on the cells being grown. Routinely cells are grown until the absorbance at 600 nm, also known as OD600, reaches approximately 0.4 prior to induction or harvesting. A linear relationship exists between cell number (density) and OD 600 up to approx. 0.6.

SmartDrop™ Nano Spectrophotometers

Microvolume Analysis of DNA, RNA and Proteins

DiluCell

Features

• Disposable cuvette for spectrophotometrical analysis
  • Protein assays, OD600


• Significantly delayed cell sedimentation
  • compared to standard cuvettes


• Automatic virtual dilution
  • Factor 10 or factor 20


• Ideal for GLP
  • No dilution - no dilution errors - no cross contamination


• Broad Wavelength range
  • 340 nm - 1100 nm


• Small sample volumes
  • 100 µl or 200 µl


• Easy handling
• Bubble-free filling
• These Cuvettes are compatible with our unit OD600, click here to see the specs

Cuvettes

Cuvettes, Visible Range Semi-Micro, 1.5 mL (Item 302205-0002)

These exceptionally fine quality polystyrene cuvettes are designed for assays throughout the 340 nm to 750 nm visible spectral range. A standard 1 cm light path and recessed windows provide maximum light transmission. Scale down your assay volumes to save on costly reagents using these specially designed cuvettes that have a minimum filling volume of 1.2 mL.

Packaged 100 per covered tray, with 500 per case.
Dimensions: 12.5 x 12.5 x 45 mm. Windows: 5 x 23 mm. Light Path: 10 mm.

Cuvettes, UV-VIS Range Semi-Micro, 1.5 mL (Item 302225-0002)

Injection molded from a superior grade of methacrylate designed for accuracy throughout the VIS-UV spectral range from 300 nm to 750 nm. Excellent optical qualities throughout the spectral range make these cuvettes perfect for scanning small volumes, enzyme rate reactions, and standard curve determinations.

Packaged 100 per covered tray, with 500 per case.
Dimensions: 12.5 x 12.5 x 45 mm. Windows: 5 x 23 mm. Light Path: 10 mm. 



Cuvettes, Visible Range Macro, 4.5 mL (Item 02195-0002)

These exceptionally fine quality polystyrene cuvettes are designed for assays throughout the 340 nm to 750 nm visible spectral range. These specially designed cuvettes handle up to
4.5 mL total volume with minimum filling volume of 2.5 mL and a maximum of 4.5 mL. Molded arrow indicates the direction of transmission for optimal signal and reproducibility.

Packaged 100 per covered tray, with 500 per case.
Dimensions: 12.5 x 12.5 x 45 mm. Window: 10 x 35 mm. Light Path: 10 mm. 

Item 685-SC
This double beam, scanning, UV/Visible spectrophotometer has a variety of programmed measurement modes for photometrics, concentration, multi-wavelength, spectrum scanning, kinetics, quantitation, DNA and protein analysis making it ideal for a number of applications.
The stable optics and two detectors measure your samples and reference simultaneously  providing superior measurement accuracy. 

The variable spectral bandwidth provides your experiments with robust detection and quantitation limits, as well as optimal data precision and repeatability. This flexible platform allows the user to comply with multiple regulatory agencies using one spectrophotometer- saving money and time. Three scan speeds are available with wavelength scan intervals of 0.1, 0.2, 1, 2 or 5 nm. The large sample chamber is outfitted with a 10 x 10 mm cuvette holder, but allows for an 8-position cuvette changer (order 83070-61 separately) for flexibility.

The integrated user interface provides local controls with the touch of a keypad. The colored graphical display is easy to read and will display spectrum as well as kinetic curves. Prism PC software is supplied with the spectrophotometer to assist the user with post-measurement analysis. The Prism PC software can be used to fully control the spectrophotometer and comes pre-loaded with methods for DNA analysis, including 260/280 and 260/230 ratios with 320 nm correction. Under the multi-wavelength measurement mode, up to 20 wavelengths can be measured simultaneously and quantitation curves can be created by measuring up to 200 standards.

Operating system compatibility: Windows 2000, XP, Vista, Windows 7
Specifications & Description

• Stray Light<0.05%T at 220 nm, 360 nm
• Beam TypeDouble
• Bandwidth0.5 nm, 1.0 nm, 2.0 nm, 4.0 nm, 5.0 nm (variable)
• Min Wavelength (nm)190
• Max Wavelength (nm)1100
• Wavelength Noise<0.0005 A
• Wavelength Accuracy±0.3 nm at 0.5 and 1 nm bandwidth; ±0.5 nm at 2, 4 and 5 nm bandwidth
• Wavelength Reproducibility±0.2 nm
• Min Photometric - Transmittance (%T)1
• Max Photometric - Transmittance (%T)200
• Min Photometric - Absorbance (A)-0.3
• Max Photometric - Absorbance (A)3
• Photometric Accuracy±0.002 A (0-0.5 A); ±0.3%T (0-100%T)

Item 632-521
This easy-to-use spectrophotometer is ideal for the busy technician or student. Wavelength calibration is automatic at start-up and includes an auto-zero (% transmittance) functiona significant time saver. This spectrophotometer is fully GLP (Good Laboratory Practice) compliant. Each sample reading is given a time and date stamp and easily connects to a printerthen simply push the print key to send data.
The included 63-Zero PC software allows you to take and control measurements remotely, and export data and results to Excel, Word, Power Point, and other software applications for storage, reporting, or presentation. Transmittance, absorbance, or concentration with factor value, as well as wavelength are simultaneously. The digital, dual-line LCD shows error messages, prompts, mode indication, and concentration units (ppm, mg/L, g/L, M, %, or blank).

Specifications & Description

The four new spectrophotometers in the Jenway 73 series range include models 7300 and 7310 which cover the visible region of the spectrum. The 7305 and 7315 models use a flash xenon lamp to extend the wavelength range into the UV region of the spectrum.

The basic models, 7300 and 7305, feature measurement modes for absorbance, % transmittance, and concentration. The advanced models, 7310 and 7315, introduce additional measurement modes such as scanning, kinetics, and quantitation.

	730-001	730-501	731-001	731-501
Weight (lbs)	13.2
Dimensions (WxLxH) (in)	10.8 x 15.7 x 8.8
Warranty	3-yr, including xenon lamp
Wavelength
Range:	320-1000nm	198-1000nm	320-1000nm	198-1000nm
Light Source:	Tungsten halogen	Xenon	Tungsten halogen	Xenon
Resolution:	1nm
Accuracy:	±2nm
Repeatability:	±0.5nm
Spectral bandwidth:	5nm
Photometrics
Transmittance:	0-199.9%
Absorbance:	-0.300 to 2.500A
Accuracy:	±0.01Abs at 1.000 Absorbance
Resolution:	0.1%T, 0.001A
Concentration
Range:	-300 to 9999
Resolution:	Selectable 1/0.1/0.01/0.001
Calibration:	Blank with single standard or factor
Factor:	0.001 to 10000
Standard:	0.001 to 1000
Quantitation:
Range:	n/a	n/a	-300 to 9999
Resolution:	n/a	n/a	Selectable 1/0.1/0.01/0.001
Calibration:	n/a	n/a	Blank with up to 6 standards
Curve fit stats:	n/a	n/a	Quadratic, quadratic through zero, linear, linear through zero, interpolate
Kinetics
Measurement time:	n/a	n/a	2 to 9999 seconds
Calibration:	n/a	n/a	Blank with a single standard or factor
Resolution:	n/a	n/a	Selectable 1/0.1/0.01/0.001
Display:	n/a	n/a	Graphical and concentration
Analysis:	n/a	n/a	Concentration, rate of change, initial and final absorbance, or % transmittance
Spectrum:
Scan interval:	n/a	n/a	Selectable 1, 2, or 5nm
Analysis:	n/a	n/a	Absorbance, or % transmittance and peak and valleys
Analysis:	n/a	n/a	Concentration, rate of change, initial and final absorbance, or % transmittance

• Manufactured from polystyrene formulated for superior clarity and transmission.
• Fits most spectrophotometers, including Beckman, Perkin-Elmer, Gilford, Hitachi, Varian, and Shimadzu.
• Packed in dust resistant trays.
• Each cuvette is packed in its own individual compartment to assure scratch-free optical surfaces.
• Polyethylene cap with flexible double seals separately available.
• These disposable polystyrene cuvettes can be used to measure samples in the 300-800nm spectral range

UNICO 2150 Series Advanced UV-Visible Spectrophotometer

• Digital interface with internal memory and auto system check
• 200-1000 nm wavelength range
• 4 nm spectral bandwidth
• 4-place holder for 10 mm square cuvettes
• USB port for optional software
• Included are 2 pcs of 10 mm square quartz cuvettes; 4 pcs of 10 mm square glass cuvettes; dust cover; user manual
• Voltage preset at 110 V

The UNICO 2150-UV Spectrophotometer offers user an economic way to UV testing without sacrificing many advanced features. With the built-in memory for up to 200 tests, expanded keyboard and large sample compartment the 2150UV is a great addition to UNICO Spectrophotometer line. Equipped standard with 4 position manual cell holder for 10 mm cells. Additional holders are available.

Specifications