Spectrophotometers

Like the wide range of wavelengths within the electromagnetic spectrum, we’ve got a wide range of spectrophotometers for a diverse set of applications.

We carry a diverse series of spectrophotometers that don’t skimp on quality. 
  
Just one of our amazing highlights is the NanoPhotometer® Touch Screen Spectrophotometer. 
  
Among its many impressive characteristics, the NanoPhotometer features full scan capabilities, an intuitive graphical user interface, and smartphone control. 
  
Moreover, the NanoPhotometer’s technology averts the need for costly and time-consuming recalibrations. 
  
This product also offers unparalleled accuracy and precision, backed by a lifetime guarantee! 
  
We’ve got this and a whole lot of other amazing spectrophotometers to choose from.

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Unico 2150UV Spectrophotometer

wavelength=200-1000nm,4nm bp

This new series of fully digital spectrophotometers are as simple and easy-to-use as they appear! The precise, 1200lines/mm grating monochromator will be welcomed by environmental, quality control, research and university teaching laboratories. A membrane keyboard logically positioned next to the large, two-line, 9-LED display allows the user to control the wavelength, set Zero Abs (100%T) automatically, and select the operation mode of either %T, Abs, or Concentration. The sample compartment accepts square cuvettes from 5mm to 100mm in length (cylindrical or rectangular) and test tubes from 10 to 25mm in diameter. The standard holder is a 4-position linear stage for 10mm path cuvettes. Optional accessories include convenient packages for long path cuvettes, test tubes, micro cuvettes, temperature control (Peltier or water bath), and automated peristaltic pumps for flowcells (with or without temperature control).

The narrow 5nm bandpass is a perfect match for the high stability and accuracy required in the wavelength ranges of either 325 to 1000nm (Model 2100), or 200 to 1000nm (Model 2100-UV). The operator has the option of controlling the instrument through our UNICO Application Software (p/n 2100-401) for PC's. This low-cost package allows for the data collection for %T/Abs/Concentration, Kinetic applications and Standard Curve with 5 different curve fits (Least Squares, Linear Thru Zero, 2nd Order, Segmented or Slope/Intercept). Personal computer not included.

1 year warranty

Item#:
S-2100UV
Your Price:
3995.00
Each

OD600 DiluPhotometer

The OD600 DiluPhotometer is a small, easy to use instrument that is dedicated to measuring samples near the 600 nm wavelength. It is suitable for measuring growth rates of several cell types including E. coli and yeast. Use of the OD600 DiluPhotometer with Implens DiluCell 10 or DiluCell 20 facilitates the measurement of low volume samples (volume requirement of 200 µl and 100 µl respectively). The two different available DiluCell 10 and DiluCell 20 versions allow for automatic 1/10 and 1/20 dilutions. With a bandwidth of 40 nm, the OD600 is also suitable for protein quantification assays like Bradford (595 nm). The small size and portable nature of the OD600 DiluPhotometer make it convenient for use in incubation cabinets and under anaerobic conditions.

The OD600 DiluPhotometer has been designed for the spectrophotometric analysis of bacterial and yeast growth rates and the quantification of proteins at a wavelength nearby 600 nm (e.g. Bradford). The instrument is battery operated and can be easily transported for usage in incubation cabinets and under anaerobic conditions. The batteries last for almost 1 month when fully charged. A 600nm LED source in combination with a fiber optic is used to obtain the optical density measurement. Spills can be easily wiped from the surface, and the cell compartment area can be rinsed with, formaldehyde or ethylene oxide to sterilize it.

 

Lambert-Beer-Law

The Lambert-Beer-Law, also known as Beers Law, empirically relates the absorption of light to the properties of the sample. This law states that there is a logarithmic relationship between the transmission of light through a specific sample (T = I/Io with I = outgoing light and Io = incoming light), the molar extinction coefficient for a specific compound (), the concentration of the absorbing species in the material (c) and the distance the light travels (d). By leaving all conditions constant but changing the pathlength (distance the light travels), you can bend the Beer-Law to get an automatic dilution of your sample. This principle is realized within the OD600 DiluPhotometer with DiluCell.

 

Automatic Sample Dilution

Previously it was necessary to use standard cuvettes with a volume of at least 1 ml and additionally to dilute the sample manually. DiluCell from Implen allows for lower sample volume requirements and provides automatic virtual sample dilution. Due to the unique design of DiluCell cuvettes, the light-path is reduced from the standard 10 mm to 0.5 mm (DC 20) or 1.0 mm (DC 10). According to the Lambert Beer Law this shortened pathlength results in an automatic sample dilution by factor 20 (DC 20) or 10 (DC 10). Automatic dilution with DiluCell saves time and excludes dilution errors and cross contaminations.

 

Measuring Cell Density

The stage of growth of a bacterial culture needs to be monitored to ensure that the cells are harvested at the optimum point (towards the end of log phase) for the greatest density of live cells. The optical density of the sample indicates when this point has been reached. This value varies dependent on the cells being grown. Routinely cells are grown until the absorbance at 600 nm, also known as OD600, reaches approximately 0.4 prior to induction or harvesting. A linear relationship exists between cell number (density) and OD 600 up to approx. 0.6.

 

Technical Specifications:

Wavelength

600nm

Bandwidth

40nm

Range

Optical Density  0.3A to 1.99A

Accuracy

< ±0.05A at 1A using Neutral Density Filters

Repeatability

±0.02A at 1A

Controls

6 button  power, blank, sample, memory, re-set, recall/print

Optical Height

8.5mm

Cuvette Holder

Fixed with drain hole. Accepts DiluCell 10, DiluCell 20, 10 mm pathlength semi micro and macro cuvettes or 14  16 mm round tubes.

Output

RS232, USB

Memory

99 readings

Display

Custom LCD

Power Requirements

External power adaptor (110 to 220 V, 50/60 Hz, 20 VA) or internal rechargeable NiMH battery

Approximate Dimensions

180 x 150 x 60mm

Weight

0.6kg

Item#:
OD600-1020
Your Price:
1499.00
Each

Type 9UV


Standard semi-micro spectrophotometer cuvette with PTFE Cover.


Features of Precision Cells Type 9 Semi-Micro Spectrophotometer Cuvette:

    • Highly accurate results, only need to test sample one time
    • Accurate repeatability, Precison Cells cuvettes are made with only the highest quality quartz and glass materials
    • Hand made in Japan, ensuring the highest quality optical polish with no defects.
    • Every cell is thoroughly inspected before leaving the factory.
    • Precision Cells cuvettes are guaranteed to give you the best optical transmission.
    • Thermally to ensure the best bonding.
    • Four different materials available: (Optical Glass, UV Quartz, ES Quartz, IR (EDC) Quartz)
    • Lightpaths from 5 mm up to 50 mm available.
    • Two windows optically polished.
    • Base Thickness = 3 mm
    • Includes PTFE cover.
Item#:
9UV10
Your Price:
123.00
Each

Spectrophotometer Model 1100RS

VIS, Single Beam, w/RS232,

This robustly built spectrophotometer combines a surprisingly high level of performance with a budget price. Wavelength is set from a simple rotary control and the instrument is ready within just a few minutes from switch-on. A membrane keyboard positioned next to the large, clear LCD display allows the operator to rapidly switch between %T and Absorbance and to set Zero Abs/100%T. The 20nm bandpass and wavelength range of 335 to 1000nm provides the accuracy required for vast applications in quality control and teaching laboratories.

Model 1100RS

This spectrophotometer shares the basics of the Model 1100, but with better accuracy and precision. The membrane keyboard positioned next to the large, clear LCD display allows the operator to rapidly switch between %T, Abs, and Concentration modes, and to automatically set Zero Abs. (100%T). The narrow 10nm bandpass is ideally matched to the wavelength range of 335 to 1000nm. Standard output includes an RS-232C for connection to a computer and the optional UNICO Application Software for Window's 9x. This low-cost program expands the capabilities to include Kinetics and Standard Curve with up to 5 calibration curve fits.

The sample compartment of the 1100 Series accepts either 10mm rectangular cuvettes, including disposable semi-micro cuvettes, or test tubes (standard). Optional holders expand the capability for COD vials or test tubes up to 1 inch in diameter. Both models are great for environmental, quality control, and teaching laboratories.

1 year warranty

Item#:
S-1100RS
Your Price:
1431.00
Each

Quartz Sub-Micro Cuvette

Type 701M, Volume .1ml, Zd 8.5

Standard 10 mm sub-micro black masking spectrophotometer cuvette with threade PTFE stopper. Get excellent results by using smaller samples and no dilution.

This sub-micro cuvette has become a favorite for researchers where sample size is a premium.


Features of Precision Cells Type 701M Sub-Micro Spectrophotometer Cuvette:

    • Highly accurate results, only need to test sample one time
    • Accurate repeatability, Precison Cells cuvettes are made with only the highest quality quartz and glass materials
    • Hand made in Japan, ensuring the highest quality optical polish with no defects.
    • Every cell is thoroughly inspected before leaving the factory.
    • Precision Cells cuvettes are guaranteed to give you the best optical transmission.
    • Thermally fused to ensure the best bonding.
    • Available only in high grade ES Quartz
    • Capacities of 10ul, 50ul, 100ul, & 160 ul. available.
    • Two windows optically polished.
    • Includes threaded PTFE stopper.
    • Great for DNA, RNA, and Protein micro samples
Item#:
QC-701M-8.5-100
Your Price:
583.50
Each

Quartz Cuvette, Sub-Micro,

Type 701M, 0.1ml, z-dim 15

Standard 10 mm sub-micro black masking spectrophotometer cuvette with threade PTFE stopper. Get excellent results by using smaller samples and no dilution.

This sub-micro cuvette has become a favorite for researchers where sample size is a premium.


Features of Precision Cells Type 701M Sub-Micro Spectrophotometer Cuvette:

    • Highly accurate results, only need to test sample one time
    • Accurate repeatability, Precison Cells cuvettes are made with only the highest quality quartz and glass materials
    • Hand made in Japan, ensuring the highest quality optical polish with no defects.
    • Every cell is thoroughly inspected before leaving the factory.
    • Precision Cells cuvettes are guaranteed to give you the best optical transmission.
    • Thermally fused to ensure the best bonding.
    • Available only in high grade ES Quartz
    • Capacities of 10ul, 50ul, 100ul, & 160 ul. available.
    • Two windows optically polished.
    • Includes threaded PTFE stopper.
    • Great for DNA, RNA, and Protein micro samples
Item#:
QC-701M-15-100
Your Price:
428.00
Each

Model SQ2800 Scanning

Spectrophotometer-Single Beam,

UNICO SQ-2800 is the most economic general-purpose design in the SpectroQuest line. It is a stand-alone model with 4nm fixed bandwidth and has all the features that the SpectroQuest line offers for a stand-alone unit. It provides excellent performance for measurements in the range of 190nm to 1100nm. It has a large angled LCD screen with contrast adjustment for comfort viewing. The large sample compartment accommodates a wide range of cell holders and accessories including peristaltic sipper and peltier system. Optional PC download software and PC Windows® application software make this instrument very versatile.

3 year warranty

 

SPECIFICATIONS

SpectroQuest 2800

Optical Specifications

Wavelength Range

190 - 1100 nm

Spectral Bandwidth

4 nm

Monochromator

Single beam Littrow type, grating 1200 lines/mm

Wavelength Accuracy

±0.8 nm

Wavelength Repeatability

±0.5 nm

Wavelength Calibration

automatic at start

Light Source

Tungsten Halogen/Deuterium  Lamp

Detector

Solid Silicon Photodiode

Photometric Readout

1/4 VGA 320X240 pixels backlit LCD

Photometric Range

0 - 200%T
-0.3 - 3.0A
0 - 9999C

Photometric Accuracy

±%0.5%T

Zero Drift

< 0.002A per hour after warm-up

Baseline Flatness

±0.004A

Stability

0.002A/h at 500 nm

Stray Light

< 0.15%T

Scanning Speed

Hi, Med., Low. Max. 1000 nm/min

Physical Characteristics

Standard Sample Holder

4-position Cell Holder

Analogue/Digital Output

Digital, RS232 Serial, Centronics Parallel

Dimensions

550(L) x 420(W) x 270(H) mm

Weight

20 kg / 44 lbs

Shipping Dimensions

650(L) x 540(W) x 390(H) mm

Shipping Weight

24 kg / 53 lbs

Power Requirements

115V/60Hz or 230V/50Hz, switchable

Operating Temperature

Room temperature: 22 ±2°C

Humidity Range

  less than 85%

Safety Certificate

CE

Item#:
SQ2800
Your Price:
5054.00
Each

The four new spectrophotometers in the Jenway 73 series range include models 7300 and 7310 which cover the visible region of the spectrum. The 7305 and 7315 models use a flash xenon lamp to extend the wavelength range into the UV region of the spectrum.

The basic models, 7300 and 7305, feature measurement modes for absorbance, % transmittance, and concentration. The advanced models, 7310 and 7315, introduce additional measurement modes such as scanning, kinetics, and quantitation.

  • 5nm spectral bandwidth
  • Absorbance range -0.3 to 2.5 Abs
  • Icon driven software
  • Results and method saving to USB memory stick (7310 and 7315 only)
  • Autologging capabilities
  • Free bi-directional PC software
  • 3 year warranty including xenon lamp
     
    730-001
    730-501
    731-001
    731-501
    Weight (lbs)
    13.2
    Dimensions (WxLxH) (in)
    10.8 x 15.7 x 8.8
    Warranty
    3-yr, including xenon lamp
     
    Wavelength
    Range:
    320-1000nm
    198-1000nm
    320-1000nm
    198-1000nm
    Light Source:
    Tungsten halogen
    Xenon
    Tungsten halogen
    Xenon
    Resolution:
    1nm
    Accuracy:
    ±2nm
    Repeatability:
    ±0.5nm
    Spectral bandwidth:
    5nm
     
    Photometrics
    Transmittance:
    0-199.9%
    Absorbance:
    -0.300 to 2.500A
    Accuracy:
    ±0.01Abs at 1.000 Absorbance
    Resolution:
    0.1%T, 0.001A
     
    Concentration
    Range:
    -300 to 9999
    Resolution:
    Selectable 1/0.1/0.01/0.001
    Calibration:
    Blank with single standard or factor
    Factor:
    0.001 to 10000
    Standard:
    0.001 to 1000
     
    Quantitation:
    Range:
    n/a
    n/a
    -300 to 9999
    Resolution:
    n/a
    n/a
    Selectable 1/0.1/0.01/0.001
    Calibration:
    n/a
    n/a
    Blank with up to 6 standards
    Curve fit stats:
    n/a
    n/a
    Quadratic, quadratic through zero, linear, linear through zero, interpolate
     
    Kinetics
    Measurement time:
    n/a
    n/a
    2 to 9999 seconds
    Calibration:
    n/a
    n/a
    Blank with a single standard or factor
    Resolution:
    n/a
    n/a
    Selectable 1/0.1/0.01/0.001
    Display:
    n/a
    n/a
    Graphical and concentration
    Analysis:
    n/a
    n/a
    Concentration, rate of change, initial and final absorbance, or % transmittance
     
    Spectrum:
    Scan interval:
    n/a
    n/a
    Selectable 1, 2, or 5nm
    Analysis:
    n/a
    n/a
    Absorbance, or % transmittance and peak and valleys
    Analysis:
    n/a
    n/a
    Concentration, rate of change, initial and final absorbance, or % transmittance
Item#:
ASBASELCT5

Item 685-SC
This double beam, scanning, UV/Visible spectrophotometer has a variety of programmed measurement modes for photometrics, concentration, multi-wavelength, spectrum scanning, kinetics, quantitation, DNA and protein analysis making it ideal for a number of applications.
The stable optics and two detectors measure your samples and reference simultaneously  providing superior measurement accuracy. 

The variable spectral bandwidth provides your experiments with robust detection and quantitation limits, as well as optimal data precision and repeatability. This flexible platform allows the user to comply with multiple regulatory agencies using one spectrophotometer- saving money and time. Three scan speeds are available with wavelength scan intervals of 0.1, 0.2, 1, 2 or 5 nm. The large sample chamber is outfitted with a 10 x 10 mm cuvette holder, but allows for an 8-position cuvette changer (order 83070-61 separately) for flexibility.

The integrated user interface provides local controls with the touch of a keypad. The colored graphical display is easy to read and will display spectrum as well as kinetic curves. Prism PC software is supplied with the spectrophotometer to assist the user with post-measurement analysis. The Prism PC software can be used to fully control the spectrophotometer and comes pre-loaded with methods for DNA analysis, including 260/280 and 260/230 ratios with 320 nm correction. Under the multi-wavelength measurement mode, up to 20 wavelengths can be measured simultaneously and quantitation curves can be created by measuring up to 200 standards.

Operating system compatibility: Windows 2000, XP, Vista, Windows 7
Specifications & Description

  • Stray Light<0.05%T at 220 nm, 360 nm
  • Beam TypeDouble
  • Bandwidth0.5 nm, 1.0 nm, 2.0 nm, 4.0 nm, 5.0 nm (variable)
  • Min Wavelength (nm)190
  • Max Wavelength (nm)1100
  • Wavelength Noise<0.0005 A
  • Wavelength Accuracy±0.3 nm at 0.5 and 1 nm bandwidth; ±0.5 nm at 2, 4 and 5 nm bandwidth
  • Wavelength Reproducibility±0.2 nm
  • Min Photometric - Transmittance (%T)1
  • Max Photometric - Transmittance (%T)200
  • Min Photometric - Absorbance (A)-0.3
  • Max Photometric - Absorbance (A)3
  • Photometric Accuracy±0.002 A (0-0.5 A); ±0.3%T (0-100%T)

Item 632-521
This easy-to-use spectrophotometer is ideal for the busy technician or student. Wavelength calibration is automatic at start-up and includes an auto-zero (% transmittance) functiona significant time saver. This spectrophotometer is fully GLP (Good Laboratory Practice) compliant. Each sample reading is given a time and date stamp and easily connects to a printerthen simply push the print key to send data.
The included 63-Zero PC software allows you to take and control measurements remotely, and export data and results to Excel, Word, Power Point, and other software applications for storage, reporting, or presentation. Transmittance, absorbance, or concentration with factor value, as well as wavelength are simultaneously. The digital, dual-line LCD shows error messages, prompts, mode indication, and concentration units (ppm, mg/L, g/L, M, %, or blank).

Specifications & Description

  •            Stray Light<0.5%T
  •            Source LampTungsten-Halogen
  •            Beam TypeSingle
  •            Bandwidth8.0 nm
  •            Min Wavelength (nm)320
  •            Max Wavelength (nm)1000
  •            Wavelength Accuracy±2.0 nm
  •            Min Photometric - Transmittance (%T)0
  •            Max Photometric - Transmittance (%T)199.9
  •            Min Photometric - Absorbance (A)-0.3
  •            Max Photometric - Absorbance (A)1.999
  •            Min Photometric - Concentration (C)-300
  •            Max Photometric - Concentration (C)1999
  •            DetectorSolid-state
  •            OutputRS-232; 0 to 1.999v
  •            Display TypeLCD (4-digit)
  •            Width (cm)26.16
  •            Height (cm)11.4

  •            Depth (cm)19.3
  • Item#:
    ASBASELCT4

    These disposable cuvettes are available in either polystyrene (VIS)or methacrylate (UV-VIS) depending on which spectral range you wish to measure. A standard 1cm light path and recessed windows provide maximum light transmission. Scale down your assay volumes to save on costly reagents using these specially designed cuvettes that have a minimum filling volume of 1.0ml.

    UV/Vis range-285nm to 750nm
            Vis range-   340nm to 750nm

    Dimensions: 12.5mm x 12.5mm x 45mm Windows: 5mm x 23mm Light Path: 10mm
    500/cs 

    Item#:
    ASBASELCT1
    MIDSCI's Macro and Semi-Micro cuvettes are specially packaged to reach you scratch- free.
    • Manufactured from polystyrene formulated for superior clarity and transmission.
    • Fits most spectrophotometers, including Beckman, Perkin-Elmer, Gilford, Hitachi, Varian, and Shimadzu.
    • Packed in dust resistant trays.
    • Each cuvette is packed in its own individual compartment to assure scratch-free optical surfaces.
    • Polyethylene cap with flexible double seals separately available.
    • These disposable polystyrene cuvettes can be used to measure samples in the 300-800nm spectral range
    Item#:
    ASBASELCT6

    Polystyrene (VIS) and Methacrylate (UV-VIS)

    Optically superior designs specifically for fluorimetry make this an excellent choice for nephelometry and spectrophotometry. A negligible variation in absorbance between the 4 optical sides allows readings from any side.

    The polystyrene cuvettes are accurate in the 340nm to 750nm visible spectral range. The methacrylate cuvettes are accurate throughout the 285nm to 750nm UV-VIS spectral range.

    Standard 1cm light path

    Item#:
    ASBASELCT2
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