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OD600 DiluPhotometer

Item #:OD600-1020 
1499.00
Each
Quantity:
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1 1499.00
OD600 Diluphotometer

OD600 DiluPhotometer

The OD600 DiluPhotometer™ is a small, easy to use instrument that is dedicated to measuring samples near the 600 nm wavelength. It is suitable for measuring growth rates of several cell types including E. coli and yeast. Use of the OD600 DiluPhotometer™ with Implen's DiluCell™ 10 or DiluCell™ 20 facilitates the measurement of low volume samples (volume requirement of 200 µl and 100 µl respectively). The two different available DiluCell™ 10 or DiluCell™ 20  versions allow for automatic 1/10 and 1/20 dilutions. With a bandwidth of 40 nm, the OD600 is also suitable for protein quantification assays like Bradford (595 nm). The small size and portable nature of the OD600 DiluPhotometer™ make it convenient for use in incubation cabinets and under anaerobic conditions.

The OD600 DiluPhotometer™ has been designed for the spectrophotometric analysis of bacterial and yeast growth rates and the quantification of proteins at a wavelength nearby 600 nm (e.g. Bradford). The instrument is battery operated and can be easily transported for usage in incubation cabinets and under anaerobic conditions. The batteries last for almost 1 month when fully charged. A 600nm LED source in combination with a fiber optic is used to obtain the optical density measurement. Spills can be easily wiped from the surface, and the cell compartment area can be rinsed with, formaldehyde or ethylene oxide to sterilize it.

 

Lambert-Beer-Law

The Lambert-Beer-Law, also known as Beers Law, empirically relates the absorption of light to the properties of the sample. This law states that there is a logarithmic relationship between the transmission of light through a specific sample (T = I/Io with I = outgoing light and Io = incoming light), the molar extinction coefficient for a specific compound (), the concentration of the absorbing species in the material (c) and the distance the light travels (d). By leaving all conditions constant but changing the pathlength (distance the light travels), you can bend the Beer-Law to get an automatic dilution of your sample. This principle is realized within the OD600 DiluPhotometer with DiluCell.

 

Automatic Sample Dilution

Previously it was necessary to use standard cuvettes with a volume of at least 1 ml and additionally to dilute the sample manually. DiluCell from Implen allows for lower sample volume requirements and provides automatic virtual sample dilution. Due to the unique design of DiluCell cuvettes, the light-path is reduced from the standard 10 mm to 0.5 mm (DC 20) or 1.0 mm (DC 10). According to the Lambert Beer Law this shortened pathlength results in an automatic sample dilution by factor 20 (DC 20) or 10 (DC 10). Automatic dilution with DiluCell saves time and excludes dilution errors and cross contaminations.

 

Measuring Cell Density

The stage of growth of a bacterial culture needs to be monitored to ensure that the cells are harvested at the optimum point (towards the end of log phase) for the greatest density of live cells. The optical density of the sample indicates when this point has been reached. This value varies dependent on the cells being grown. Routinely cells are grown until the absorbance at 600 nm, also known as OD600, reaches approximately 0.4 prior to induction or harvesting. A linear relationship exists between cell number (density) and OD 600 up to approx. 0.6.

 

Technical Specifications:

Wavelength

600nm

Bandwidth

40nm

Range

Optical Density  0.3A to 1.99A

Accuracy

< ±0.05A at 1A using Neutral Density Filters

Repeatability

±0.02A at 1A

Controls

6 button  power, blank, sample, memory, re-set, recall/print

Optical Height

8.5mm

Cuvette Holder

Fixed with drain hole. Accepts DiluCell 10, DiluCell 20, 10 mm pathlength semi micro and macro cuvettes or 14  16 mm round tubes.

Output

RS232, USB

Memory

99 readings

Display

Custom LCD

Power Requirements

External power adaptor (110 to 220 V, 50/60 Hz, 20 VA) or internal rechargeable NiMH battery

Approximate Dimensions

180 x 150 x 60mm

Weight

0.6kg

PNDILUPH

Range
Optical Density - 0.3 to 1.99A
Wavelength
600nm
Battery
Chargeable
Dilutions
1/10, 1/20 available
OD600 Diluphotometer
OD600 DiluPhotometer
1499.00
Each
On Order
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