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FroggaMark Protein ladder

500ul
Item #:FPL1 
139.78
Each
Quantity:
Quantity Web Price
1 139.78
FroggaMark Protein Ladder

Affordable price & shipped at room temperature.

Supplied in gel loading buffer and ready to use.

  • The FroggaMark Protein Ladder is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights (from 10 to 245 kDa).
  • Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).
  • It is designed for promoting separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

 

Recommended Load:

  • 3 µL or 5 µL per loading for clear visualization during electrophoresis in 15-well or 10-well mini-gel, respectively.
  • 1.5-2.5 µL per well for general Western transferring.
  • Apply more for thicker (>1.5mm) or larger gel.

Contents: Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Trisphosphate, pH 7.5 at 25°C), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea and 15% (v/v) Glycerol.


Quality Control: Under suggested conditions, the FroggaMark Protein Ladder resolves 12 major bands in 15% SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocellulose membrane.

Storage:

  • Stable for up to 2 weeks at 25°C
  • Store for up to 3 months at 4°C
  • For long term storage, store at -20°C

PNBLOTBX2

Size
500 uL
FroggaMark Protein Ladder
FroggaMark Protein ladder
139.78
Each
Quantity: