Bullseye Taq DNA Polymerase

New and Improved! Ideal for General PCR, Genomic analysis and TA cloning!

Bulleye Taq DNA Polymerase is the most popular thermostable enzyme used in DNA amplification experiments. This high performance Taq DNA polymerase is specifically purified to produce excellent yields with little or no background. Its outstanding activity and unique thermal DNA amplification properties make it one of the best-value DNA polymerases available.

• Robust performance
• Leaves 3' A overhang
• Stable at all storage temperatures
• Ideal for general PCR, genomic analysis and TA cloning
• This product is not recommended for work with neo-primers

Storage: -20°C

Quality Control:
Taq DNA Polymerase is highly purified, free of contaminating endonucleases, exonucleases and nicking activity. For endonuclease assay, 1µg of Lamda/Hind III DNA is incubated with 20 units of enzyme in assay buffer at 75oC for 16 hours with no visible contaminating activity observed. Also, every lot is tested for its performance consistency.

Unit Definition:
One unit incorporates 10nmoles of 4 radioactive labeled dNTPs into acid-insoluble material in 30 minutes at 74°C.

Storage Buffer:
5 units/µl in 50mM Tris-HCl (pH8.0), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 50% glycerol, 0.5% TritonX-100, and 0.5% NP-40.
10x Reaction Buffer 100mM KCl, 100mM Tris HCl (pH9.0), 80mM (NH4)2SO4, and 1.0% Triton X-100. (Mg++free): is optimized for use with 200µM dNTPs.

Magnesium Chloride:
25mM MgCl2: In general, 1.5mM MgCl2 is recommended; this may vary with different conditions and primer sets.

Please give us a call for a sample.

For laboratory research only. Not for clinical applications.